Experiment: HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02670 and A4249_RS02675 are separated by 60 nucleotides A4249_RS02675 and A4249_RS02680 are separated by 38 nucleotides A4249_RS02680 and A4249_RS02685 overlap by 4 nucleotides A4249_RS02685 and A4249_RS02690 overlap by 4 nucleotides
A4249_RS02670: A4249_RS02670 - enoyl-CoA hydratase, at 570,954 to 571,727
_RS02670
A4249_RS02675: A4249_RS02675 - N-acetyltransferase, at 571,788 to 572,282
_RS02675
A4249_RS02680: A4249_RS02680 - DUF1285 domain-containing protein, at 572,321 to 572,866
_RS02680
A4249_RS02685: A4249_RS02685 - CoA pyrophosphatase, at 572,863 to 573,525
_RS02685
A4249_RS02690: A4249_RS02690 - CCA tRNA nucleotidyltransferase, at 573,522 to 574,736
_RS02690
Position (kb)
572
573 Strain fitness (log2 ratio)
-2
-1
0
1 at 571.479 kb on - strand, within A4249_RS02670 at 571.725 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.808 kb on - strand at 571.819 kb on + strand at 571.957 kb on + strand, within A4249_RS02675 at 571.958 kb on - strand, within A4249_RS02675 at 572.157 kb on + strand, within A4249_RS02675 at 572.158 kb on - strand, within A4249_RS02675 at 572.309 kb on + strand at 572.309 kb on + strand at 572.310 kb on - strand at 572.310 kb on - strand at 572.310 kb on - strand at 572.326 kb on + strand at 572.326 kb on + strand at 572.435 kb on - strand, within A4249_RS02680 at 572.435 kb on - strand, within A4249_RS02680 at 572.499 kb on + strand, within A4249_RS02680 at 572.499 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.777 kb on + strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 572.778 kb on - strand, within A4249_RS02680 at 573.149 kb on + strand, within A4249_RS02685 at 573.149 kb on + strand, within A4249_RS02685 at 573.150 kb on - strand, within A4249_RS02685 at 573.217 kb on + strand, within A4249_RS02685 at 573.217 kb on + strand, within A4249_RS02685 at 573.217 kb on + strand, within A4249_RS02685 at 573.217 kb on + strand, within A4249_RS02685 at 573.218 kb on - strand, within A4249_RS02685 at 573.218 kb on - strand, within A4249_RS02685 at 573.218 kb on - strand, within A4249_RS02685 at 573.367 kb on + strand, within A4249_RS02685 at 573.367 kb on + strand, within A4249_RS02685 at 573.367 kb on + strand, within A4249_RS02685 at 573.368 kb on - strand, within A4249_RS02685 at 573.368 kb on - strand, within A4249_RS02685 at 573.424 kb on + strand, within A4249_RS02685 at 573.424 kb on + strand, within A4249_RS02685 at 573.425 kb on - strand, within A4249_RS02685 at 573.473 kb on + strand at 573.473 kb on + strand at 573.474 kb on - strand at 573.474 kb on - strand at 573.474 kb on - strand at 573.474 kb on - strand at 573.475 kb on + strand at 573.475 kb on + strand at 573.476 kb on - strand at 573.476 kb on - strand at 573.476 kb on - strand at 573.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) remove 571,479 - A4249_RS02670 0.68 -0.8 571,725 + -0.0 571,773 + +0.3 571,773 + +0.0 571,773 + +0.8 571,773 + -0.8 571,774 - -0.6 571,774 - -0.9 571,774 - +0.5 571,774 - +0.4 571,774 - +0.4 571,807 + -0.3 571,807 + -0.1 571,807 + +0.1 571,807 + -0.0 571,807 + +0.3 571,808 - -0.6 571,819 + -0.1 571,957 + A4249_RS02675 0.34 -0.9 571,958 - A4249_RS02675 0.34 -0.4 572,157 + A4249_RS02675 0.75 -0.9 572,158 - A4249_RS02675 0.75 +0.4 572,309 + -0.7 572,309 + -0.2 572,310 - -0.4 572,310 - -0.6 572,310 - -1.0 572,326 + -0.1 572,326 + -0.1 572,435 - A4249_RS02680 0.21 +1.4 572,435 - A4249_RS02680 0.21 -0.9 572,499 + A4249_RS02680 0.33 -0.7 572,499 + A4249_RS02680 0.33 -0.4 572,777 + A4249_RS02680 0.84 -1.5 572,777 + A4249_RS02680 0.84 -0.7 572,777 + A4249_RS02680 0.84 -0.2 572,777 + A4249_RS02680 0.84 -2.4 572,777 + A4249_RS02680 0.84 -0.5 572,777 + A4249_RS02680 0.84 +0.2 572,777 + A4249_RS02680 0.84 +0.3 572,777 + A4249_RS02680 0.84 +0.2 572,778 - A4249_RS02680 0.84 +1.1 572,778 - A4249_RS02680 0.84 -1.3 572,778 - A4249_RS02680 0.84 -0.2 572,778 - A4249_RS02680 0.84 -0.1 572,778 - A4249_RS02680 0.84 -0.1 572,778 - A4249_RS02680 0.84 -0.1 573,149 + A4249_RS02685 0.43 +0.7 573,149 + A4249_RS02685 0.43 -0.6 573,150 - A4249_RS02685 0.43 -0.2 573,217 + A4249_RS02685 0.53 +0.1 573,217 + A4249_RS02685 0.53 -0.5 573,217 + A4249_RS02685 0.53 -1.0 573,217 + A4249_RS02685 0.53 +0.2 573,218 - A4249_RS02685 0.54 +1.1 573,218 - A4249_RS02685 0.54 +0.8 573,218 - A4249_RS02685 0.54 +0.3 573,367 + A4249_RS02685 0.76 -0.4 573,367 + A4249_RS02685 0.76 -0.4 573,367 + A4249_RS02685 0.76 +0.2 573,368 - A4249_RS02685 0.76 -0.1 573,368 - A4249_RS02685 0.76 -0.5 573,424 + A4249_RS02685 0.85 +0.1 573,424 + A4249_RS02685 0.85 -0.2 573,425 - A4249_RS02685 0.85 -0.3 573,473 + -0.2 573,473 + +0.0 573,474 - -1.7 573,474 - -0.2 573,474 - +1.2 573,474 - -0.2 573,475 + +0.9 573,475 + -0.8 573,476 - -1.4 573,476 - -0.3 573,476 - -0.1 573,476 - -0.5
Or see this region's nucleotide sequence