Experiment: HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02525 and A4249_RS02530 are separated by 22 nucleotides A4249_RS02530 and A4249_RS02535 overlap by 4 nucleotides A4249_RS02535 and A4249_RS02540 are separated by 79 nucleotides A4249_RS02540 and A4249_RS02545 overlap by 4 nucleotides
A4249_RS02525: A4249_RS02525 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 541,660 to 542,475
_RS02525
A4249_RS02530: A4249_RS02530 - GMP synthase, at 542,498 to 543,229
_RS02530
A4249_RS02535: A4249_RS02535 - putative sulfate exporter family transporter, at 543,226 to 544,284
_RS02535
A4249_RS02540: A4249_RS02540 - LysR family transcriptional regulator, at 544,364 to 545,239
_RS02540
A4249_RS02545: A4249_RS02545 - oxygen-dependent coproporphyrinogen oxidase, at 545,236 to 546,117
_RS02545
Position (kb) 543
544
545 Strain fitness (log2 ratio) -1
0
1
2 at 542.486 kb on + strand at 542.486 kb on + strand at 542.486 kb on + strand at 542.486 kb on + strand at 542.487 kb on - strand at 542.487 kb on - strand at 542.487 kb on - strand at 542.696 kb on + strand, within A4249_RS02530 at 542.696 kb on + strand, within A4249_RS02530 at 542.697 kb on - strand, within A4249_RS02530 at 542.697 kb on - strand, within A4249_RS02530 at 542.697 kb on - strand, within A4249_RS02530 at 542.697 kb on - strand, within A4249_RS02530 at 542.858 kb on + strand, within A4249_RS02530 at 542.858 kb on + strand, within A4249_RS02530 at 542.858 kb on + strand, within A4249_RS02530 at 542.983 kb on + strand, within A4249_RS02530 at 542.984 kb on - strand, within A4249_RS02530 at 542.991 kb on - strand, within A4249_RS02530 at 543.056 kb on + strand, within A4249_RS02530 at 543.056 kb on + strand, within A4249_RS02530 at 543.056 kb on + strand, within A4249_RS02530 at 543.056 kb on + strand, within A4249_RS02530 at 543.056 kb on + strand, within A4249_RS02530 at 543.057 kb on - strand, within A4249_RS02530 at 543.057 kb on - strand, within A4249_RS02530 at 543.057 kb on - strand, within A4249_RS02530 at 543.057 kb on - strand, within A4249_RS02530 at 543.057 kb on - strand, within A4249_RS02530 at 543.227 kb on + strand at 543.227 kb on + strand at 543.228 kb on - strand at 543.460 kb on + strand, within A4249_RS02535 at 543.460 kb on + strand, within A4249_RS02535 at 543.460 kb on + strand, within A4249_RS02535 at 543.460 kb on + strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.461 kb on - strand, within A4249_RS02535 at 543.632 kb on - strand, within A4249_RS02535 at 543.632 kb on - strand, within A4249_RS02535 at 543.678 kb on - strand, within A4249_RS02535 at 543.735 kb on + strand, within A4249_RS02535 at 544.331 kb on + strand at 544.331 kb on + strand at 544.331 kb on + strand at 544.331 kb on + strand at 544.331 kb on + strand at 544.384 kb on + strand at 544.384 kb on + strand at 544.384 kb on + strand at 544.384 kb on + strand at 544.384 kb on + strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.385 kb on - strand at 544.673 kb on + strand, within A4249_RS02540 at 544.673 kb on + strand, within A4249_RS02540 at 544.673 kb on + strand, within A4249_RS02540 at 544.673 kb on + strand, within A4249_RS02540 at 544.674 kb on - strand, within A4249_RS02540 at 544.674 kb on - strand, within A4249_RS02540 at 544.712 kb on + strand, within A4249_RS02540 at 544.712 kb on + strand, within A4249_RS02540 at 544.713 kb on - strand, within A4249_RS02540 at 544.863 kb on + strand, within A4249_RS02540 at 544.863 kb on + strand, within A4249_RS02540 at 544.863 kb on + strand, within A4249_RS02540 at 544.863 kb on + strand, within A4249_RS02540 at 544.866 kb on + strand, within A4249_RS02540 at 544.866 kb on + strand, within A4249_RS02540 at 544.866 kb on + strand, within A4249_RS02540 at 544.866 kb on + strand, within A4249_RS02540 at 544.866 kb on + strand, within A4249_RS02540 at 544.867 kb on - strand, within A4249_RS02540 at 544.867 kb on - strand, within A4249_RS02540 at 544.867 kb on - strand, within A4249_RS02540 at 544.867 kb on - strand, within A4249_RS02540 at 544.867 kb on - strand, within A4249_RS02540 at 545.108 kb on + strand, within A4249_RS02540 at 545.108 kb on + strand, within A4249_RS02540 at 545.108 kb on + strand, within A4249_RS02540 at 545.108 kb on + strand, within A4249_RS02540 at 545.109 kb on - strand, within A4249_RS02540 at 545.109 kb on - strand, within A4249_RS02540 at 545.109 kb on - strand, within A4249_RS02540 at 545.109 kb on - strand, within A4249_RS02540 at 545.109 kb on - strand, within A4249_RS02540 at 545.178 kb on + strand at 545.178 kb on + strand at 545.178 kb on + strand at 545.178 kb on + strand at 545.179 kb on - strand at 545.179 kb on - strand at 545.195 kb on + strand at 545.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) remove 542,486 + +0.1 542,486 + +0.2 542,486 + -0.6 542,486 + +0.3 542,487 - -0.4 542,487 - +0.1 542,487 - -0.6 542,696 + A4249_RS02530 0.27 +0.1 542,696 + A4249_RS02530 0.27 -0.6 542,697 - A4249_RS02530 0.27 -0.8 542,697 - A4249_RS02530 0.27 -0.4 542,697 - A4249_RS02530 0.27 -0.1 542,697 - A4249_RS02530 0.27 +0.1 542,858 + A4249_RS02530 0.49 +0.9 542,858 + A4249_RS02530 0.49 +0.8 542,858 + A4249_RS02530 0.49 +0.4 542,983 + A4249_RS02530 0.66 -1.7 542,984 - A4249_RS02530 0.66 -0.4 542,991 - A4249_RS02530 0.67 -0.1 543,056 + A4249_RS02530 0.76 +0.5 543,056 + A4249_RS02530 0.76 -0.6 543,056 + A4249_RS02530 0.76 -0.7 543,056 + A4249_RS02530 0.76 -0.6 543,056 + A4249_RS02530 0.76 +0.1 543,057 - A4249_RS02530 0.76 +0.0 543,057 - A4249_RS02530 0.76 +1.6 543,057 - A4249_RS02530 0.76 +0.5 543,057 - A4249_RS02530 0.76 -1.5 543,057 - A4249_RS02530 0.76 +0.4 543,227 + +1.0 543,227 + -0.5 543,228 - -0.4 543,460 + A4249_RS02535 0.22 -1.2 543,460 + A4249_RS02535 0.22 +0.2 543,460 + A4249_RS02535 0.22 +0.4 543,460 + A4249_RS02535 0.22 -1.3 543,461 - A4249_RS02535 0.22 +2.0 543,461 - A4249_RS02535 0.22 +0.8 543,461 - A4249_RS02535 0.22 +0.3 543,461 - A4249_RS02535 0.22 +0.6 543,461 - A4249_RS02535 0.22 -0.8 543,461 - A4249_RS02535 0.22 +0.5 543,461 - A4249_RS02535 0.22 +0.1 543,461 - A4249_RS02535 0.22 -0.5 543,632 - A4249_RS02535 0.38 -0.3 543,632 - A4249_RS02535 0.38 +0.2 543,678 - A4249_RS02535 0.43 -0.3 543,735 + A4249_RS02535 0.48 -0.6 544,331 + +0.5 544,331 + +0.3 544,331 + -1.4 544,331 + -0.6 544,331 + +0.4 544,384 + +0.4 544,384 + +1.8 544,384 + -0.0 544,384 + -0.2 544,384 + -0.2 544,385 - +0.2 544,385 - +0.3 544,385 - -0.4 544,385 - +0.4 544,385 - +1.4 544,385 - -0.6 544,385 - +0.8 544,385 - +0.3 544,673 + A4249_RS02540 0.35 -0.2 544,673 + A4249_RS02540 0.35 -0.3 544,673 + A4249_RS02540 0.35 +0.6 544,673 + A4249_RS02540 0.35 -0.4 544,674 - A4249_RS02540 0.35 -0.3 544,674 - A4249_RS02540 0.35 -0.1 544,712 + A4249_RS02540 0.40 -1.6 544,712 + A4249_RS02540 0.40 -0.2 544,713 - A4249_RS02540 0.40 -0.8 544,863 + A4249_RS02540 0.57 -0.3 544,863 + A4249_RS02540 0.57 +0.4 544,863 + A4249_RS02540 0.57 -0.9 544,863 + A4249_RS02540 0.57 +0.2 544,866 + A4249_RS02540 0.57 -0.1 544,866 + A4249_RS02540 0.57 +0.4 544,866 + A4249_RS02540 0.57 -0.5 544,866 + A4249_RS02540 0.57 -0.9 544,866 + A4249_RS02540 0.57 +0.1 544,867 - A4249_RS02540 0.57 +0.8 544,867 - A4249_RS02540 0.57 -0.5 544,867 - A4249_RS02540 0.57 +0.4 544,867 - A4249_RS02540 0.57 +0.1 544,867 - A4249_RS02540 0.57 +0.5 545,108 + A4249_RS02540 0.85 +0.1 545,108 + A4249_RS02540 0.85 -0.2 545,108 + A4249_RS02540 0.85 +0.2 545,108 + A4249_RS02540 0.85 -0.5 545,109 - A4249_RS02540 0.85 +0.8 545,109 - A4249_RS02540 0.85 +1.1 545,109 - A4249_RS02540 0.85 -0.0 545,109 - A4249_RS02540 0.85 -0.7 545,109 - A4249_RS02540 0.85 -0.5 545,178 + -1.7 545,178 + +0.1 545,178 + -1.3 545,178 + -1.5 545,179 - +0.9 545,179 - -0.0 545,195 + -1.7 545,238 - +0.4
Or see this region's nucleotide sequence