Experiment: HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01530 and A4249_RS16190 overlap by 80 nucleotides A4249_RS16190 and A4249_RS01535 are separated by 111 nucleotides A4249_RS01535 and A4249_RS01540 overlap by 4 nucleotides A4249_RS01540 and A4249_RS01545 are separated by 12 nucleotides A4249_RS01545 and A4249_RS01550 are separated by 15 nucleotides
A4249_RS01530: A4249_RS01530 - mechanosensitive ion channel family protein, at 285,640 to 286,815
_RS01530
A4249_RS16190: A4249_RS16190 - endonuclease domain-containing protein, at 286,736 to 287,083
_RS16190
A4249_RS01535: A4249_RS01535 - hypothetical protein, at 287,195 to 287,608
_RS01535
A4249_RS01540: A4249_RS01540 - chromosome segregation protein SMC, at 287,605 to 291,042
_RS01540
A4249_RS01545: A4249_RS01545 - thioredoxin domain-containing protein, at 291,055 to 291,666
_RS01545
A4249_RS01550: A4249_RS01550 - DsbA family protein, at 291,682 to 292,326
_RS01550
Position (kb) 287
288
289
290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 286.737 kb on + strand at 286.737 kb on + strand at 286.737 kb on + strand at 286.737 kb on + strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.738 kb on - strand at 286.813 kb on + strand at 286.814 kb on - strand at 286.814 kb on - strand at 286.859 kb on + strand, within A4249_RS16190 at 286.860 kb on - strand, within A4249_RS16190 at 286.874 kb on + strand, within A4249_RS16190 at 286.875 kb on - strand, within A4249_RS16190 at 286.875 kb on - strand, within A4249_RS16190 at 286.939 kb on + strand, within A4249_RS16190 at 286.939 kb on + strand, within A4249_RS16190 at 286.939 kb on + strand, within A4249_RS16190 at 286.940 kb on - strand, within A4249_RS16190 at 286.940 kb on - strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.989 kb on + strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.990 kb on - strand, within A4249_RS16190 at 286.993 kb on - strand, within A4249_RS16190 at 287.196 kb on + strand at 287.196 kb on + strand at 287.196 kb on + strand at 287.196 kb on + strand at 287.197 kb on - strand at 287.197 kb on - strand at 287.197 kb on - strand at 287.197 kb on - strand at 287.319 kb on + strand, within A4249_RS01535 at 287.319 kb on + strand, within A4249_RS01535 at 287.320 kb on - strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.442 kb on + strand, within A4249_RS01535 at 287.443 kb on - strand, within A4249_RS01535 at 287.443 kb on - strand, within A4249_RS01535 at 287.443 kb on - strand, within A4249_RS01535 at 287.538 kb on + strand, within A4249_RS01535 at 287.538 kb on + strand, within A4249_RS01535 at 287.538 kb on + strand, within A4249_RS01535 at 287.538 kb on + strand, within A4249_RS01535 at 287.539 kb on - strand, within A4249_RS01535 at 287.539 kb on - strand, within A4249_RS01535 at 287.539 kb on - strand, within A4249_RS01535 at 287.539 kb on - strand, within A4249_RS01535 at 287.684 kb on + strand at 287.685 kb on - strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.948 kb on + strand at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 287.949 kb on - strand, within A4249_RS01540 at 288.182 kb on + strand, within A4249_RS01540 at 288.183 kb on - strand, within A4249_RS01540 at 288.251 kb on + strand, within A4249_RS01540 at 288.502 kb on - strand, within A4249_RS01540 at 288.502 kb on - strand, within A4249_RS01540 at 288.553 kb on + strand, within A4249_RS01540 at 288.553 kb on + strand, within A4249_RS01540 at 288.554 kb on - strand, within A4249_RS01540 at 289.224 kb on - strand, within A4249_RS01540 at 289.224 kb on - strand, within A4249_RS01540 at 289.415 kb on + strand, within A4249_RS01540 at 289.415 kb on + strand, within A4249_RS01540 at 289.416 kb on - strand, within A4249_RS01540 at 289.484 kb on + strand, within A4249_RS01540 at 289.484 kb on + strand, within A4249_RS01540 at 289.485 kb on - strand, within A4249_RS01540 at 289.639 kb on + strand, within A4249_RS01540 at 289.640 kb on - strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.342 kb on + strand, within A4249_RS01540 at 290.343 kb on - strand, within A4249_RS01540 at 290.343 kb on - strand, within A4249_RS01540 at 290.343 kb on - strand, within A4249_RS01540 at 290.343 kb on - strand, within A4249_RS01540 at 290.343 kb on - strand, within A4249_RS01540 at 290.390 kb on + strand, within A4249_RS01540 at 290.390 kb on + strand, within A4249_RS01540 at 290.391 kb on - strand, within A4249_RS01540 at 290.391 kb on - strand, within A4249_RS01540 at 290.470 kb on + strand, within A4249_RS01540 at 290.470 kb on + strand, within A4249_RS01540 at 290.470 kb on + strand, within A4249_RS01540 at 290.470 kb on + strand, within A4249_RS01540 at 290.470 kb on + strand, within A4249_RS01540 at 290.471 kb on - strand, within A4249_RS01540 at 290.471 kb on - strand, within A4249_RS01540 at 290.980 kb on - strand at 290.980 kb on - strand at 291.056 kb on + strand at 291.056 kb on + strand at 291.108 kb on + strand at 291.109 kb on - strand at 291.335 kb on + strand, within A4249_RS01545 at 291.336 kb on - strand, within A4249_RS01545 at 291.336 kb on - strand, within A4249_RS01545 at 291.442 kb on - strand, within A4249_RS01545 at 291.442 kb on - strand, within A4249_RS01545 at 291.442 kb on - strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.443 kb on + strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.444 kb on - strand, within A4249_RS01545 at 291.512 kb on + strand, within A4249_RS01545 at 291.512 kb on + strand, within A4249_RS01545 at 291.512 kb on + strand, within A4249_RS01545 at 291.513 kb on - strand, within A4249_RS01545 at 291.552 kb on + strand, within A4249_RS01545 at 291.552 kb on + strand, within A4249_RS01545 at 291.553 kb on - strand, within A4249_RS01545 at 291.553 kb on - strand, within A4249_RS01545 at 291.593 kb on - strand, within A4249_RS01545 at 291.684 kb on - strand at 291.684 kb on - strand at 291.684 kb on - strand at 291.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) remove 286,737 + +0.4 286,737 + +0.7 286,737 + +0.6 286,737 + -0.2 286,738 - +0.9 286,738 - +0.8 286,738 - -0.8 286,738 - +0.3 286,738 - +0.3 286,738 - +0.3 286,738 - -0.2 286,738 - +2.0 286,813 + +0.9 286,814 - -0.3 286,814 - -0.2 286,859 + A4249_RS16190 0.35 -0.1 286,860 - A4249_RS16190 0.36 +0.4 286,874 + A4249_RS16190 0.40 -0.2 286,875 - A4249_RS16190 0.40 -0.6 286,875 - A4249_RS16190 0.40 +0.9 286,939 + A4249_RS16190 0.58 -1.3 286,939 + A4249_RS16190 0.58 -1.7 286,939 + A4249_RS16190 0.58 +0.6 286,940 - A4249_RS16190 0.59 +1.0 286,940 - A4249_RS16190 0.59 -0.8 286,989 + A4249_RS16190 0.73 -0.0 286,989 + A4249_RS16190 0.73 +0.4 286,989 + A4249_RS16190 0.73 +1.4 286,989 + A4249_RS16190 0.73 -0.2 286,989 + A4249_RS16190 0.73 +0.7 286,989 + A4249_RS16190 0.73 -0.2 286,989 + A4249_RS16190 0.73 +0.3 286,989 + A4249_RS16190 0.73 +0.1 286,989 + A4249_RS16190 0.73 -0.3 286,989 + A4249_RS16190 0.73 +0.6 286,989 + A4249_RS16190 0.73 +0.4 286,989 + A4249_RS16190 0.73 -1.6 286,989 + A4249_RS16190 0.73 +0.0 286,989 + A4249_RS16190 0.73 +0.5 286,990 - A4249_RS16190 0.73 -0.4 286,990 - A4249_RS16190 0.73 -1.8 286,990 - A4249_RS16190 0.73 -1.0 286,990 - A4249_RS16190 0.73 +0.7 286,990 - A4249_RS16190 0.73 +0.3 286,990 - A4249_RS16190 0.73 -1.2 286,990 - A4249_RS16190 0.73 -0.3 286,990 - A4249_RS16190 0.73 -1.7 286,990 - A4249_RS16190 0.73 -1.2 286,990 - A4249_RS16190 0.73 +0.3 286,990 - A4249_RS16190 0.73 -0.2 286,990 - A4249_RS16190 0.73 -0.9 286,990 - A4249_RS16190 0.73 +0.2 286,990 - A4249_RS16190 0.73 +0.0 286,990 - A4249_RS16190 0.73 +0.6 286,990 - A4249_RS16190 0.73 +0.1 286,990 - A4249_RS16190 0.73 -1.4 286,990 - A4249_RS16190 0.73 +0.1 286,990 - A4249_RS16190 0.73 -0.5 286,993 - A4249_RS16190 0.74 +1.1 287,196 + +0.2 287,196 + +0.2 287,196 + -1.4 287,196 + -1.6 287,197 - -0.2 287,197 - +0.2 287,197 - -0.1 287,197 - -1.7 287,319 + A4249_RS01535 0.30 -0.2 287,319 + A4249_RS01535 0.30 +0.2 287,320 - A4249_RS01535 0.30 +0.1 287,442 + A4249_RS01535 0.60 +0.7 287,442 + A4249_RS01535 0.60 -1.1 287,442 + A4249_RS01535 0.60 -0.5 287,442 + A4249_RS01535 0.60 +1.0 287,442 + A4249_RS01535 0.60 -0.1 287,442 + A4249_RS01535 0.60 +1.9 287,442 + A4249_RS01535 0.60 -0.3 287,442 + A4249_RS01535 0.60 -0.1 287,442 + A4249_RS01535 0.60 -0.4 287,442 + A4249_RS01535 0.60 +0.4 287,442 + A4249_RS01535 0.60 -0.4 287,442 + A4249_RS01535 0.60 +0.1 287,443 - A4249_RS01535 0.60 +1.3 287,443 - A4249_RS01535 0.60 -0.1 287,443 - A4249_RS01535 0.60 +2.4 287,538 + A4249_RS01535 0.83 +0.8 287,538 + A4249_RS01535 0.83 +0.3 287,538 + A4249_RS01535 0.83 -0.3 287,538 + A4249_RS01535 0.83 -0.7 287,539 - A4249_RS01535 0.83 -1.0 287,539 - A4249_RS01535 0.83 -0.5 287,539 - A4249_RS01535 0.83 -1.2 287,539 - A4249_RS01535 0.83 +0.2 287,684 + -0.6 287,685 - +0.3 287,948 + -0.8 287,948 + +0.8 287,948 + -2.0 287,948 + +0.3 287,948 + +1.9 287,948 + -1.0 287,948 + +0.4 287,948 + -0.6 287,948 + -1.1 287,948 + -0.3 287,948 + -1.0 287,948 + +2.1 287,948 + +1.1 287,948 + -0.8 287,948 + +0.5 287,948 + +0.0 287,948 + +0.0 287,948 + +0.3 287,948 + +0.6 287,948 + -0.7 287,948 + -0.6 287,949 - A4249_RS01540 0.10 +0.6 287,949 - A4249_RS01540 0.10 -0.6 287,949 - A4249_RS01540 0.10 -0.4 287,949 - A4249_RS01540 0.10 +1.1 287,949 - A4249_RS01540 0.10 -0.1 287,949 - A4249_RS01540 0.10 -0.2 287,949 - A4249_RS01540 0.10 +0.6 287,949 - A4249_RS01540 0.10 -0.2 287,949 - A4249_RS01540 0.10 -0.0 287,949 - A4249_RS01540 0.10 +1.4 287,949 - A4249_RS01540 0.10 -0.4 287,949 - A4249_RS01540 0.10 +0.9 287,949 - A4249_RS01540 0.10 +0.8 287,949 - A4249_RS01540 0.10 +0.7 287,949 - A4249_RS01540 0.10 +1.9 287,949 - A4249_RS01540 0.10 -1.7 288,182 + A4249_RS01540 0.17 -2.0 288,183 - A4249_RS01540 0.17 +0.7 288,251 + A4249_RS01540 0.19 +1.3 288,502 - A4249_RS01540 0.26 -0.3 288,502 - A4249_RS01540 0.26 -0.9 288,553 + A4249_RS01540 0.28 -0.7 288,553 + A4249_RS01540 0.28 +0.4 288,554 - A4249_RS01540 0.28 -0.7 289,224 - A4249_RS01540 0.47 -2.0 289,224 - A4249_RS01540 0.47 -0.0 289,415 + A4249_RS01540 0.53 +0.6 289,415 + A4249_RS01540 0.53 -0.4 289,416 - A4249_RS01540 0.53 +0.0 289,484 + A4249_RS01540 0.55 -0.9 289,484 + A4249_RS01540 0.55 -1.0 289,485 - A4249_RS01540 0.55 +0.0 289,639 + A4249_RS01540 0.59 -0.6 289,640 - A4249_RS01540 0.59 +0.5 290,342 + A4249_RS01540 0.80 -0.1 290,342 + A4249_RS01540 0.80 +0.6 290,342 + A4249_RS01540 0.80 -1.2 290,342 + A4249_RS01540 0.80 +0.1 290,342 + A4249_RS01540 0.80 -0.6 290,342 + A4249_RS01540 0.80 +0.9 290,342 + A4249_RS01540 0.80 -1.3 290,343 - A4249_RS01540 0.80 +0.7 290,343 - A4249_RS01540 0.80 +0.4 290,343 - A4249_RS01540 0.80 +0.8 290,343 - A4249_RS01540 0.80 +0.7 290,343 - A4249_RS01540 0.80 -1.2 290,390 + A4249_RS01540 0.81 +0.4 290,390 + A4249_RS01540 0.81 -0.5 290,391 - A4249_RS01540 0.81 +1.5 290,391 - A4249_RS01540 0.81 -0.6 290,470 + A4249_RS01540 0.83 +0.0 290,470 + A4249_RS01540 0.83 -0.3 290,470 + A4249_RS01540 0.83 -0.6 290,470 + A4249_RS01540 0.83 -0.3 290,470 + A4249_RS01540 0.83 +0.5 290,471 - A4249_RS01540 0.83 +0.5 290,471 - A4249_RS01540 0.83 -1.4 290,980 - +0.9 290,980 - -0.9 291,056 + -0.8 291,056 + -0.2 291,108 + +0.3 291,109 - -0.2 291,335 + A4249_RS01545 0.46 -2.9 291,336 - A4249_RS01545 0.46 -0.3 291,336 - A4249_RS01545 0.46 +0.8 291,442 - A4249_RS01545 0.63 +0.2 291,442 - A4249_RS01545 0.63 -0.5 291,442 - A4249_RS01545 0.63 +2.1 291,443 + A4249_RS01545 0.63 -0.2 291,443 + A4249_RS01545 0.63 -0.2 291,443 + A4249_RS01545 0.63 +0.0 291,443 + A4249_RS01545 0.63 +0.4 291,443 + A4249_RS01545 0.63 +2.3 291,443 + A4249_RS01545 0.63 -0.1 291,443 + A4249_RS01545 0.63 +0.4 291,443 + A4249_RS01545 0.63 +1.2 291,444 - A4249_RS01545 0.64 +0.7 291,444 - A4249_RS01545 0.64 +0.7 291,444 - A4249_RS01545 0.64 -0.4 291,444 - A4249_RS01545 0.64 +0.5 291,444 - A4249_RS01545 0.64 +0.1 291,444 - A4249_RS01545 0.64 -0.8 291,444 - A4249_RS01545 0.64 +0.7 291,444 - A4249_RS01545 0.64 -0.2 291,444 - A4249_RS01545 0.64 -2.5 291,444 - A4249_RS01545 0.64 -0.5 291,444 - A4249_RS01545 0.64 +0.3 291,512 + A4249_RS01545 0.75 +0.1 291,512 + A4249_RS01545 0.75 +1.2 291,512 + A4249_RS01545 0.75 -1.2 291,513 - A4249_RS01545 0.75 -0.4 291,552 + A4249_RS01545 0.81 -0.3 291,552 + A4249_RS01545 0.81 -1.6 291,553 - A4249_RS01545 0.81 -0.4 291,553 - A4249_RS01545 0.81 +1.0 291,593 - A4249_RS01545 0.88 -1.1 291,684 - -0.1 291,684 - -0.1 291,684 - +0.8 291,684 - -0.9
Or see this region's nucleotide sequence