Strain Fitness in Variovorax sp. SCN45 around GFF932

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF931 and GFF932 are separated by 10 nucleotidesGFF932 and GFF933 overlap by 7 nucleotides GFF931 - Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2), at 243,377 to 245,539 GFF931 GFF932 - Acetyltransferase, GNAT family, at 245,550 to 246,026 GFF932 GFF933 - YgfD: protein that forms a complex with the methylmalonyl-CoA mutase in a pathway for conversion of succinyl-CoA to propionyl-CoA, at 246,020 to 247,069 GFF933 Position (kb) 245 246 247Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 244.586 kb on + strand, within GFF931at 244.586 kb on + strand, within GFF931at 244.586 kb on + strand, within GFF931at 244.586 kb on + strand, within GFF931at 244.586 kb on + strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.587 kb on - strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.775 kb on + strand, within GFF931at 244.776 kb on - strand, within GFF931at 244.776 kb on - strand, within GFF931at 244.776 kb on - strand, within GFF931at 244.776 kb on - strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.060 kb on + strand, within GFF931at 245.061 kb on - strand, within GFF931at 245.061 kb on - strand, within GFF931at 245.072 kb on + strand, within GFF931at 245.429 kb on + strandat 245.430 kb on - strandat 245.442 kb on - strandat 245.465 kb on + strandat 245.465 kb on + strandat 245.465 kb on + strandat 245.466 kb on - strandat 245.466 kb on - strandat 245.466 kb on - strandat 245.715 kb on + strand, within GFF932at 245.715 kb on + strand, within GFF932at 245.715 kb on + strand, within GFF932at 245.715 kb on + strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.716 kb on - strand, within GFF932at 245.808 kb on + strand, within GFF932at 245.808 kb on + strand, within GFF932at 245.809 kb on - strand, within GFF932at 245.937 kb on + strand, within GFF932at 245.985 kb on + strandat 245.985 kb on + strandat 245.985 kb on + strandat 245.985 kb on + strandat 245.986 kb on - strandat 245.986 kb on - strandat 245.988 kb on + strandat 245.988 kb on + strandat 245.988 kb on + strandat 246.377 kb on + strand, within GFF933at 246.377 kb on + strand, within GFF933at 246.378 kb on - strand, within GFF933at 246.381 kb on + strand, within GFF933at 246.381 kb on + strand, within GFF933at 246.381 kb on + strand, within GFF933at 246.381 kb on + strand, within GFF933at 246.382 kb on - strand, within GFF933at 246.382 kb on - strand, within GFF933at 246.382 kb on - strand, within GFF933at 246.382 kb on - strand, within GFF933at 246.382 kb on - strand, within GFF933

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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244,586 + GFF931 0.56 -0.3
244,586 + GFF931 0.56 -1.0
244,586 + GFF931 0.56 -0.6
244,586 + GFF931 0.56 +3.1
244,586 + GFF931 0.56 +0.5
244,587 - GFF931 0.56 -0.4
244,587 - GFF931 0.56 -0.5
244,587 - GFF931 0.56 +0.4
244,587 - GFF931 0.56 -1.0
244,587 - GFF931 0.56 -0.4
244,587 - GFF931 0.56 -0.3
244,775 + GFF931 0.65 -0.6
244,775 + GFF931 0.65 +0.4
244,775 + GFF931 0.65 -0.1
244,775 + GFF931 0.65 -0.1
244,775 + GFF931 0.65 -0.2
244,775 + GFF931 0.65 -0.2
244,775 + GFF931 0.65 +0.3
244,776 - GFF931 0.65 -0.8
244,776 - GFF931 0.65 +1.0
244,776 - GFF931 0.65 +1.5
244,776 - GFF931 0.65 +1.5
245,060 + GFF931 0.78 -3.2
245,060 + GFF931 0.78 -0.5
245,060 + GFF931 0.78 +0.1
245,060 + GFF931 0.78 -0.2
245,060 + GFF931 0.78 +0.1
245,060 + GFF931 0.78 -1.7
245,061 - GFF931 0.78 -1.3
245,061 - GFF931 0.78 -0.8
245,072 + GFF931 0.78 +0.9
245,429 + +0.9
245,430 - +0.1
245,442 - -0.8
245,465 + -0.1
245,465 + +0.3
245,465 + +0.1
245,466 - +0.2
245,466 - +0.5
245,466 - -1.2
245,715 + GFF932 0.35 -0.5
245,715 + GFF932 0.35 +0.5
245,715 + GFF932 0.35 +0.5
245,715 + GFF932 0.35 -0.6
245,716 - GFF932 0.35 -0.4
245,716 - GFF932 0.35 -0.3
245,716 - GFF932 0.35 -0.3
245,716 - GFF932 0.35 -0.8
245,716 - GFF932 0.35 +0.3
245,716 - GFF932 0.35 -1.4
245,716 - GFF932 0.35 -1.5
245,808 + GFF932 0.54 -0.5
245,808 + GFF932 0.54 +0.5
245,809 - GFF932 0.54 -1.0
245,937 + GFF932 0.81 -0.2
245,985 + +0.5
245,985 + +1.0
245,985 + +0.0
245,985 + -0.3
245,986 - +0.2
245,986 - +0.2
245,988 + +0.0
245,988 + +0.5
245,988 + -0.5
246,377 + GFF933 0.34 +1.2
246,377 + GFF933 0.34 +0.0
246,378 - GFF933 0.34 +0.2
246,381 + GFF933 0.34 +0.0
246,381 + GFF933 0.34 -0.9
246,381 + GFF933 0.34 -0.4
246,381 + GFF933 0.34 +0.2
246,382 - GFF933 0.34 -0.0
246,382 - GFF933 0.34 -0.5
246,382 - GFF933 0.34 -0.2
246,382 - GFF933 0.34 +0.3
246,382 - GFF933 0.34 +0.3

Or see this region's nucleotide sequence