Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5913 and GFF5914 overlap by 4 nucleotides GFF5914 and GFF5915 are separated by 18 nucleotides GFF5915 and GFF5916 are separated by 71 nucleotides
GFF5913 - T6SS outer membrane component TssL (ImpK/VasF) / unknown domain, at 128,254 to 129,597
GFF5913
GFF5914 - hypothetical protein, at 129,594 to 130,706
GFF5914
GFF5915 - T6SS secretion lipoprotein TssJ (VasD), at 130,725 to 131,237
GFF5915
GFF5916 - T6SS component TssA (ImpA), at 131,309 to 132,349
GFF5916
Position (kb) 129
130
131 Strain fitness (log2 ratio) -1
0
1
2
3 at 128.626 kb on + strand, within GFF5913 at 128.663 kb on - strand, within GFF5913 at 128.663 kb on - strand, within GFF5913 at 128.785 kb on + strand, within GFF5913 at 128.786 kb on - strand, within GFF5913 at 128.884 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.280 kb on + strand, within GFF5913 at 129.281 kb on - strand, within GFF5913 at 129.295 kb on + strand, within GFF5913 at 129.506 kb on - strand at 130.230 kb on + strand, within GFF5914 at 130.230 kb on + strand, within GFF5914 at 130.230 kb on + strand, within GFF5914 at 130.231 kb on - strand, within GFF5914 at 130.726 kb on + strand at 131.020 kb on + strand, within GFF5915 at 131.021 kb on - strand, within GFF5915 at 131.206 kb on + strand at 131.223 kb on - strand at 131.282 kb on + strand at 131.487 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.640 kb on + strand, within GFF5916 at 131.641 kb on - strand, within GFF5916
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 128,626 + GFF5913 0.28 +0.4 128,663 - GFF5913 0.30 -0.4 128,663 - GFF5913 0.30 -0.4 128,785 + GFF5913 0.40 -0.8 128,786 - GFF5913 0.40 +1.0 128,884 + GFF5913 0.47 -0.3 129,280 + GFF5913 0.76 -0.3 129,280 + GFF5913 0.76 +2.5 129,280 + GFF5913 0.76 +0.3 129,281 - GFF5913 0.76 +0.9 129,295 + GFF5913 0.77 +1.3 129,506 - -0.6 130,230 + GFF5914 0.57 -1.1 130,230 + GFF5914 0.57 -0.8 130,230 + GFF5914 0.57 -1.2 130,231 - GFF5914 0.57 -0.0 130,726 + +2.9 131,020 + GFF5915 0.58 +0.0 131,021 - GFF5915 0.58 -0.2 131,206 + -0.7 131,223 - +0.4 131,282 + -0.6 131,487 + GFF5916 0.17 +2.6 131,640 + GFF5916 0.32 -0.4 131,640 + GFF5916 0.32 -0.4 131,640 + GFF5916 0.32 +0.4 131,641 - GFF5916 0.32 -0.7
Or see this region's nucleotide sequence