Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4304 and GFF4305 are separated by 232 nucleotides GFF4305 and GFF4306 overlap by 4 nucleotides GFF4306 and GFF4307 are separated by 1 nucleotides
GFF4304 - Cyclohexanecarboxylate--CoA ligase, at 223,480 to 225,141
GFF4304
GFF4305 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 225,374 to 226,198
GFF4305
GFF4306 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 226,195 to 227,067
GFF4306
GFF4307 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 227,069 to 228,109
GFF4307
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1 at 224.698 kb on + strand, within GFF4304 at 224.723 kb on - strand, within GFF4304 at 224.761 kb on + strand, within GFF4304 at 224.779 kb on + strand, within GFF4304 at 224.990 kb on - strand at 224.990 kb on - strand at 224.990 kb on - strand at 225.019 kb on + strand at 225.250 kb on - strand at 225.768 kb on - strand, within GFF4305 at 226.342 kb on + strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.343 kb on - strand, within GFF4306 at 226.345 kb on + strand, within GFF4306 at 226.753 kb on + strand, within GFF4306 at 226.961 kb on - strand, within GFF4306 at 227.195 kb on + strand, within GFF4307
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 224,698 + GFF4304 0.73 -0.7 224,723 - GFF4304 0.75 +1.0 224,761 + GFF4304 0.77 +0.5 224,779 + GFF4304 0.78 -0.7 224,990 - -0.4 224,990 - -1.2 224,990 - -0.3 225,019 + -0.9 225,250 - +0.5 225,768 - GFF4305 0.48 -2.7 226,342 + GFF4306 0.17 -0.3 226,343 - GFF4306 0.17 +0.1 226,343 - GFF4306 0.17 +0.7 226,343 - GFF4306 0.17 -0.4 226,345 + GFF4306 0.17 -0.0 226,753 + GFF4306 0.64 +0.0 226,961 - GFF4306 0.88 +0.0 227,195 + GFF4307 0.12 -0.3
Or see this region's nucleotide sequence