Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4299 and GFF4300 overlap by 1 nucleotides GFF4300 and GFF4301 are separated by 139 nucleotides
GFF4299 - 3-ketoacyl-CoA thiolase, at 218,005 to 219,210
GFF4299
GFF4300 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) / conserved protein associated with acetyl-CoA C-acyltransferase, at 219,210 to 220,691
GFF4300
GFF4301 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 220,831 to 221,745
GFF4301
Position (kb) 219
220
221 Strain fitness (log2 ratio) -1
0
1 at 218.580 kb on - strand, within GFF4299 at 218.747 kb on + strand, within GFF4299 at 219.254 kb on + strand at 219.281 kb on - strand at 219.499 kb on + strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.718 kb on + strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 220.069 kb on + strand, within GFF4300 at 220.612 kb on - strand at 220.695 kb on + strand at 220.722 kb on + strand at 221.270 kb on - strand, within GFF4301 at 221.594 kb on - strand, within GFF4301
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 218,580 - GFF4299 0.48 +0.7 218,747 + GFF4299 0.62 -0.2 219,254 + -0.7 219,281 - -0.3 219,499 + GFF4300 0.20 +1.3 219,500 - GFF4300 0.20 +0.6 219,500 - GFF4300 0.20 -0.2 219,500 - GFF4300 0.20 +0.4 219,718 + GFF4300 0.34 +1.7 219,719 - GFF4300 0.34 +1.0 219,719 - GFF4300 0.34 -0.1 219,719 - GFF4300 0.34 +0.0 220,069 + GFF4300 0.58 -0.3 220,612 - +0.9 220,695 + +1.1 220,722 + -0.3 221,270 - GFF4301 0.48 -0.1 221,594 - GFF4301 0.83 -0.6
Or see this region's nucleotide sequence