Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3927 and GFF3928 are separated by 161 nucleotides GFF3928 and GFF3929 are separated by 96 nucleotides GFF3929 and GFF3930 are separated by 15 nucleotides
GFF3927 - N-acetylornithine aminotransferase (EC 2.6.1.11), at 131,959 to 133,167
GFF3927
GFF3928 - Histone acetyltransferase HPA2 and related acetyltransferases, at 133,329 to 133,739
GFF3928
GFF3929 - SSU ribosomal protein S20p, at 133,836 to 134,150
GFF3929
GFF3930 - Peptidoglycan lipid II flippase MurJ, at 134,166 to 135,833
GFF3930
Position (kb) 133
134 Strain fitness (log2 ratio) -2
-1
0
1 at 133.183 kb on - strand at 133.183 kb on - strand at 133.198 kb on + strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.238 kb on - strand at 133.279 kb on + strand at 133.280 kb on - strand at 133.467 kb on + strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.764 kb on + strand at 134.200 kb on + strand at 134.201 kb on - strand at 134.201 kb on - strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.267 kb on - strand at 134.267 kb on - strand at 134.267 kb on - strand at 134.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 133,183 - -0.6 133,183 - +0.3 133,198 + -0.2 133,199 - -0.3 133,199 - -0.3 133,199 - -0.2 133,199 - -0.3 133,238 - -0.4 133,279 + -0.2 133,280 - -2.6 133,467 + GFF3928 0.34 -0.4 133,468 - GFF3928 0.34 +0.6 133,468 - GFF3928 0.34 +0.6 133,468 - GFF3928 0.34 -0.9 133,477 - GFF3928 0.36 -1.2 133,477 - GFF3928 0.36 -2.2 133,477 - GFF3928 0.36 -0.9 133,764 + -0.9 134,200 + -0.5 134,201 - -0.7 134,201 - -0.5 134,266 + -0.7 134,266 + -0.4 134,266 + +0.6 134,266 + +0.4 134,267 - -1.2 134,267 - -0.5 134,267 - -0.5 134,267 - +0.4
Or see this region's nucleotide sequence