Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3713 and GFF3714 are separated by 116 nucleotides GFF3714 and GFF3715 are separated by 14 nucleotides GFF3715 and GFF3716 overlap by 4 nucleotides GFF3716 and GFF3717 are separated by 8 nucleotides
GFF3713 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 212,159 to 212,866
GFF3713
GFF3714 - FMN reductase (NADH) RutF (EC 1.5.1.42), at 212,983 to 213,531
GFF3714
GFF3715 - Probable malonic semialdehyde reductase RutE (EC 1.1.1.298), at 213,546 to 214,145
GFF3715
GFF3716 - Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway, at 214,142 to 214,948
GFF3716
GFF3717 - Putative aminoacrylate peracid reductase RutC @ RidA/YER057c/UK114 superfamily protein, at 214,957 to 215,349
GFF3717
Position (kb) 213
214
215 Strain fitness (log2 ratio) -2
-1
0
1 at 212.744 kb on + strand, within GFF3713 at 212.744 kb on + strand, within GFF3713 at 213.029 kb on + strand at 213.030 kb on - strand at 213.856 kb on + strand, within GFF3715 at 214.027 kb on + strand, within GFF3715 at 214.027 kb on + strand, within GFF3715 at 214.028 kb on - strand, within GFF3715 at 214.150 kb on + strand at 214.522 kb on - strand, within GFF3716 at 214.758 kb on + strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 215.037 kb on - strand, within GFF3717
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 212,744 + GFF3713 0.83 -0.0 212,744 + GFF3713 0.83 -0.8 213,029 + +0.6 213,030 - -2.2 213,856 + GFF3715 0.52 +1.2 214,027 + GFF3715 0.80 -0.8 214,027 + GFF3715 0.80 -0.8 214,028 - GFF3715 0.80 -1.0 214,150 + -0.2 214,522 - GFF3716 0.47 +0.2 214,758 + GFF3716 0.76 -0.5 214,759 - GFF3716 0.76 +0.6 214,759 - GFF3716 0.76 -0.1 214,759 - GFF3716 0.76 -0.4 215,037 - GFF3717 0.20 +0.1
Or see this region's nucleotide sequence