Strain Fitness in Variovorax sp. SCN45 around GFF2701

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2699 and GFF2700 are separated by 28 nucleotidesGFF2700 and GFF2701 overlap by 4 nucleotidesGFF2701 and GFF2702 overlap by 4 nucleotides GFF2699 - 4Fe-4S ferredoxin, iron-sulfur binding, at 127,254 to 127,589 GFF2699 GFF2700 - Enoyl-CoA hydratase (EC 4.2.1.17), at 127,618 to 128,394 GFF2700 GFF2701 - CaiB/BaiF family protein, at 128,391 to 129,560 GFF2701 GFF2702 - FAD-dependent oxidoreductase, at 129,557 to 130,939 GFF2702 Position (kb) 128 129 130Strain fitness (log2 ratio) -1 0 1 2at 127.423 kb on + strand, within GFF2699at 127.489 kb on + strand, within GFF2699at 127.511 kb on - strand, within GFF2699at 127.511 kb on - strand, within GFF2699at 127.597 kb on - strandat 127.620 kb on - strandat 128.741 kb on - strand, within GFF2701at 129.184 kb on + strand, within GFF2701at 129.184 kb on + strand, within GFF2701at 129.184 kb on + strand, within GFF2701at 129.856 kb on - strand, within GFF2702at 129.858 kb on + strand, within GFF2702at 129.859 kb on - strand, within GFF2702at 129.859 kb on - strand, within GFF2702at 129.862 kb on - strand, within GFF2702at 129.862 kb on - strand, within GFF2702at 129.930 kb on + strand, within GFF2702at 130.296 kb on + strand, within GFF2702at 130.297 kb on - strand, within GFF2702at 130.297 kb on - strand, within GFF2702at 130.297 kb on - strand, within GFF2702

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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127,423 + GFF2699 0.50 -1.6
127,489 + GFF2699 0.70 +1.3
127,511 - GFF2699 0.76 -0.4
127,511 - GFF2699 0.76 -0.0
127,597 - +2.4
127,620 - -0.5
128,741 - GFF2701 0.30 -1.2
129,184 + GFF2701 0.68 -0.7
129,184 + GFF2701 0.68 -0.5
129,184 + GFF2701 0.68 -1.6
129,856 - GFF2702 0.22 +0.1
129,858 + GFF2702 0.22 +0.9
129,859 - GFF2702 0.22 +0.9
129,859 - GFF2702 0.22 -0.6
129,862 - GFF2702 0.22 -0.6
129,862 - GFF2702 0.22 +0.5
129,930 + GFF2702 0.27 +0.0
130,296 + GFF2702 0.53 -0.1
130,297 - GFF2702 0.54 -0.1
130,297 - GFF2702 0.54 -0.5
130,297 - GFF2702 0.54 +1.1

Or see this region's nucleotide sequence