Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF206 and GFF207 are separated by 11 nucleotides GFF207 and GFF208 overlap by 4 nucleotides GFF208 and GFF209 are separated by 77 nucleotides
GFF206 - GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2), at 233,157 to 234,773
GFF206
GFF207 - Death on curing protein, Doc toxin, at 234,785 to 235,219
GFF207
GFF208 - Prevent host death protein, Phd antitoxin, at 235,216 to 235,464
GFF208
GFF209 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 235,542 to 237,011
GFF209
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1 at 234.894 kb on + strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.906 kb on + strand, within GFF207 at 234.907 kb on - strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.498 kb on + strand at 235.543 kb on + strand at 235.543 kb on + strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.556 kb on - strand at 235.556 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 234,894 + GFF207 0.25 +0.3 234,895 - GFF207 0.25 +0.1 234,895 - GFF207 0.25 +1.6 234,906 + GFF207 0.28 +1.0 234,907 - GFF207 0.28 -0.7 235,137 + GFF207 0.81 -0.1 235,137 + GFF207 0.81 -0.4 235,137 + GFF207 0.81 +0.2 235,137 + GFF207 0.81 -0.1 235,138 - GFF207 0.81 -0.6 235,138 - GFF207 0.81 +0.3 235,456 - -0.0 235,456 - -1.1 235,456 - -1.6 235,456 - -1.6 235,456 - +1.4 235,456 - +0.1 235,456 - -0.6 235,456 - -1.3 235,498 + -0.3 235,543 + +0.6 235,543 + +0.9 235,544 - +0.3 235,544 - -0.3 235,544 - -1.4 235,544 - -0.7 235,555 + -1.1 235,555 + +0.8 235,555 + -0.2 235,555 + -1.3 235,556 - -1.9 235,556 - -0.9
Or see this region's nucleotide sequence