Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1671 and GFF1672 overlap by 4 nucleotides GFF1672 and GFF1673 are separated by 30 nucleotides GFF1673 and GFF1674 overlap by 4 nucleotides
GFF1671 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 316,589 to 317,863
GFF1671
GFF1672 - BUG/TctC family periplasmic protein, at 317,860 to 318,825
GFF1672
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
GFF1674 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 319,563 to 320,324
GFF1674
Position (kb) 317
318
319 Strain fitness (log2 ratio) -1
0
1
2 at 316.965 kb on + strand, within GFF1671 at 316.992 kb on + strand, within GFF1671 at 317.451 kb on + strand, within GFF1671 at 317.451 kb on + strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.452 kb on - strand, within GFF1671 at 317.565 kb on + strand, within GFF1671 at 317.566 kb on - strand, within GFF1671 at 317.649 kb on + strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.650 kb on - strand, within GFF1671 at 317.879 kb on + strand at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.315 kb on - strand, within GFF1672 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 319.203 kb on + strand, within GFF1673 at 319.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 316,965 + GFF1671 0.29 +2.2 316,992 + GFF1671 0.32 +0.2 317,451 + GFF1671 0.68 +0.2 317,451 + GFF1671 0.68 -0.1 317,452 - GFF1671 0.68 +0.8 317,452 - GFF1671 0.68 -0.2 317,452 - GFF1671 0.68 -0.1 317,565 + GFF1671 0.77 -1.5 317,566 - GFF1671 0.77 +1.2 317,649 + GFF1671 0.83 -0.0 317,650 - GFF1671 0.83 -0.5 317,650 - GFF1671 0.83 -0.2 317,650 - GFF1671 0.83 +0.2 317,879 + +0.3 318,279 - GFF1672 0.43 -1.4 318,279 - GFF1672 0.43 -0.1 318,279 - GFF1672 0.43 +0.8 318,315 - GFF1672 0.47 +0.3 318,947 + GFF1673 0.13 -0.7 318,947 + GFF1673 0.13 +0.4 318,947 + GFF1673 0.13 -0.2 319,203 + GFF1673 0.49 -0.6 319,621 + +0.8
Or see this region's nucleotide sequence