Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1601 and GFF1602 are separated by 23 nucleotides GFF1602 and GFF1603 overlap by 4 nucleotides GFF1603 and GFF1604 are separated by 1 nucleotides
GFF1601 - no description, at 199,020 to 200,219
GFF1601
GFF1602 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 200,243 to 200,965
GFF1602
GFF1603 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 200,962 to 201,729
GFF1603
GFF1604 - Prolidase (EC 3.4.13.9), at 201,731 to 202,996
GFF1604
Position (kb) 200
201
202 Strain fitness (log2 ratio) -1
0
1 at 200.213 kb on + strand at 200.652 kb on + strand, within GFF1602 at 200.652 kb on + strand, within GFF1602 at 200.985 kb on - strand at 200.985 kb on - strand at 201.042 kb on - strand, within GFF1603 at 201.758 kb on + strand at 202.302 kb on + strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.432 kb on + strand, within GFF1604 at 202.433 kb on - strand, within GFF1604 at 202.433 kb on - strand, within GFF1604
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 200,213 + -0.2 200,652 + GFF1602 0.57 -0.2 200,652 + GFF1602 0.57 +0.5 200,985 - +0.2 200,985 - -0.2 201,042 - GFF1603 0.10 +0.4 201,758 + +0.2 202,302 + GFF1604 0.45 +0.1 202,303 - GFF1604 0.45 -0.7 202,303 - GFF1604 0.45 -0.3 202,320 + GFF1604 0.47 -1.0 202,320 + GFF1604 0.47 +0.0 202,320 + GFF1604 0.47 +0.2 202,321 - GFF1604 0.47 -1.0 202,321 - GFF1604 0.47 +0.2 202,321 - GFF1604 0.47 +1.0 202,321 - GFF1604 0.47 -0.9 202,432 + GFF1604 0.55 +0.0 202,433 - GFF1604 0.55 -1.0 202,433 - GFF1604 0.55 +0.2
Or see this region's nucleotide sequence