Strain Fitness in Variovorax sp. SCN45 around GFF127

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF125 and GFF126 are separated by 32 nucleotidesGFF126 and GFF127 are separated by 23 nucleotidesGFF127 and GFF128 are separated by 31 nucleotidesGFF128 and GFF129 are separated by 180 nucleotides GFF125 - Dihydroorotase (EC 3.5.2.3), at 153,927 to 154,979 GFF125 GFF126 - Repressor homolog, at 155,012 to 155,920 GFF126 GFF127 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 155,944 to 156,627 GFF127 GFF128 - hypothetical protein, at 156,659 to 156,985 GFF128 GFF129 - Ankyrin, at 157,166 to 158,812 GFF129 Position (kb) 155 156 157Strain fitness (log2 ratio) -2 -1 0 1at 154.975 kb on + strandat 154.976 kb on - strandat 154.977 kb on + strandat 154.978 kb on - strandat 154.978 kb on - strandat 154.978 kb on - strandat 154.978 kb on - strandat 155.238 kb on + strand, within GFF126at 155.239 kb on - strand, within GFF126at 155.258 kb on + strand, within GFF126at 155.258 kb on + strand, within GFF126at 155.259 kb on - strand, within GFF126at 155.367 kb on - strand, within GFF126at 155.864 kb on + strandat 155.864 kb on + strandat 156.253 kb on + strand, within GFF127at 156.254 kb on - strand, within GFF127at 156.254 kb on - strand, within GFF127at 156.625 kb on + strandat 156.728 kb on + strand, within GFF128at 156.728 kb on + strand, within GFF128at 156.729 kb on - strand, within GFF128at 156.779 kb on + strand, within GFF128at 156.809 kb on + strand, within GFF128at 156.809 kb on + strand, within GFF128at 156.810 kb on - strand, within GFF128at 156.810 kb on - strand, within GFF128at 156.821 kb on + strand, within GFF128at 156.822 kb on - strand, within GFF128at 156.822 kb on - strand, within GFF128at 156.959 kb on + strandat 156.959 kb on + strandat 156.959 kb on + strandat 157.469 kb on + strand, within GFF129at 157.469 kb on + strand, within GFF129at 157.469 kb on + strand, within GFF129at 157.470 kb on - strand, within GFF129at 157.470 kb on - strand, within GFF129at 157.565 kb on + strand, within GFF129at 157.566 kb on - strand, within GFF129

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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154,975 + -1.1
154,976 - -0.5
154,977 + -0.2
154,978 - -1.1
154,978 - -0.2
154,978 - -0.6
154,978 - -0.1
155,238 + GFF126 0.25 -2.1
155,239 - GFF126 0.25 +1.0
155,258 + GFF126 0.27 -0.5
155,258 + GFF126 0.27 +0.6
155,259 - GFF126 0.27 -0.6
155,367 - GFF126 0.39 +0.1
155,864 + -0.1
155,864 + +0.2
156,253 + GFF127 0.45 -0.6
156,254 - GFF127 0.45 -0.9
156,254 - GFF127 0.45 -1.3
156,625 + +1.2
156,728 + GFF128 0.21 -0.5
156,728 + GFF128 0.21 -0.9
156,729 - GFF128 0.21 -0.0
156,779 + GFF128 0.37 +0.2
156,809 + GFF128 0.46 -0.6
156,809 + GFF128 0.46 +0.7
156,810 - GFF128 0.46 +0.1
156,810 - GFF128 0.46 -0.2
156,821 + GFF128 0.50 -0.7
156,822 - GFF128 0.50 -0.9
156,822 - GFF128 0.50 -1.4
156,959 + -1.3
156,959 + -0.0
156,959 + -0.2
157,469 + GFF129 0.18 -0.0
157,469 + GFF129 0.18 +0.0
157,469 + GFF129 0.18 +0.1
157,470 - GFF129 0.18 -0.5
157,470 - GFF129 0.18 +0.7
157,565 + GFF129 0.24 -0.4
157,566 - GFF129 0.24 +0.8

Or see this region's nucleotide sequence