Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS07660 and A4249_RS07665 are separated by 22 nucleotides A4249_RS07665 and A4249_RS07670 are separated by 21 nucleotides A4249_RS07670 and A4249_RS07675 are separated by 159 nucleotides
A4249_RS07660: A4249_RS07660 - 5-dehydro-4-deoxy-D-glucuronate isomerase, at 1,526,390 to 1,527,235
_RS07660
A4249_RS07665: A4249_RS07665 - 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD, at 1,527,258 to 1,528,016
_RS07665
A4249_RS07670: A4249_RS07670 - sugar kinase, at 1,528,038 to 1,529,081
_RS07670
A4249_RS07675: A4249_RS07675 - pectate lyase, at 1,529,241 to 1,530,884
_RS07675
Position (kb) 1528
1529
1530 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1527.133 kb on + strand, within A4249_RS07660 at 1527.133 kb on + strand, within A4249_RS07660 at 1527.155 kb on + strand at 1527.155 kb on + strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.156 kb on - strand at 1527.194 kb on + strand at 1527.195 kb on - strand at 1527.206 kb on + strand at 1527.206 kb on + strand at 1527.206 kb on + strand at 1527.207 kb on - strand at 1527.207 kb on - strand at 1527.726 kb on + strand, within A4249_RS07665 at 1527.726 kb on + strand, within A4249_RS07665 at 1527.727 kb on - strand, within A4249_RS07665 at 1527.727 kb on - strand, within A4249_RS07665 at 1527.966 kb on + strand at 1527.966 kb on + strand at 1527.967 kb on - strand at 1527.967 kb on - strand at 1528.025 kb on + strand at 1528.210 kb on + strand, within A4249_RS07670 at 1528.211 kb on - strand, within A4249_RS07670 at 1528.320 kb on + strand, within A4249_RS07670 at 1528.320 kb on + strand, within A4249_RS07670 at 1528.320 kb on + strand, within A4249_RS07670 at 1528.321 kb on - strand, within A4249_RS07670 at 1528.365 kb on + strand, within A4249_RS07670 at 1528.365 kb on + strand, within A4249_RS07670 at 1528.365 kb on + strand, within A4249_RS07670 at 1528.365 kb on + strand, within A4249_RS07670 at 1528.366 kb on - strand, within A4249_RS07670 at 1528.395 kb on + strand, within A4249_RS07670 at 1528.395 kb on + strand, within A4249_RS07670 at 1528.395 kb on + strand, within A4249_RS07670 at 1528.396 kb on - strand, within A4249_RS07670 at 1528.396 kb on - strand, within A4249_RS07670 at 1528.545 kb on + strand, within A4249_RS07670 at 1528.546 kb on - strand, within A4249_RS07670 at 1528.546 kb on - strand, within A4249_RS07670 at 1528.546 kb on - strand, within A4249_RS07670 at 1528.570 kb on + strand, within A4249_RS07670 at 1528.571 kb on - strand, within A4249_RS07670 at 1528.571 kb on - strand, within A4249_RS07670 at 1528.571 kb on - strand, within A4249_RS07670 at 1528.784 kb on + strand, within A4249_RS07670 at 1528.868 kb on + strand, within A4249_RS07670 at 1528.868 kb on + strand, within A4249_RS07670 at 1528.868 kb on + strand, within A4249_RS07670 at 1528.868 kb on + strand, within A4249_RS07670 at 1528.869 kb on - strand, within A4249_RS07670 at 1528.869 kb on - strand, within A4249_RS07670 at 1528.869 kb on - strand, within A4249_RS07670 at 1529.038 kb on + strand at 1529.038 kb on + strand at 1529.039 kb on - strand at 1529.039 kb on - strand at 1529.204 kb on + strand at 1529.205 kb on - strand at 1529.415 kb on + strand, within A4249_RS07675 at 1529.416 kb on - strand, within A4249_RS07675 at 1529.416 kb on - strand, within A4249_RS07675 at 1529.427 kb on + strand, within A4249_RS07675 at 1529.427 kb on + strand, within A4249_RS07675 at 1529.427 kb on + strand, within A4249_RS07675 at 1529.427 kb on + strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.428 kb on - strand, within A4249_RS07675 at 1529.535 kb on + strand, within A4249_RS07675 at 1529.535 kb on + strand, within A4249_RS07675 at 1529.536 kb on - strand, within A4249_RS07675 at 1529.536 kb on - strand, within A4249_RS07675 at 1529.560 kb on - strand, within A4249_RS07675 at 1529.562 kb on + strand, within A4249_RS07675 at 1529.563 kb on - strand, within A4249_RS07675 at 1529.563 kb on - strand, within A4249_RS07675 at 1529.563 kb on - strand, within A4249_RS07675 at 1529.563 kb on - strand, within A4249_RS07675 at 1529.563 kb on - strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.628 kb on + strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.629 kb on - strand, within A4249_RS07675 at 1529.670 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.718 kb on + strand, within A4249_RS07675 at 1529.719 kb on - strand, within A4249_RS07675 at 1529.719 kb on - strand, within A4249_RS07675 at 1529.721 kb on + strand, within A4249_RS07675 at 1529.721 kb on + strand, within A4249_RS07675 at 1529.722 kb on - strand, within A4249_RS07675 at 1529.784 kb on + strand, within A4249_RS07675 at 1529.784 kb on + strand, within A4249_RS07675 at 1529.784 kb on + strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.785 kb on - strand, within A4249_RS07675 at 1529.808 kb on + strand, within A4249_RS07675 at 1529.808 kb on + strand, within A4249_RS07675 at 1529.808 kb on + strand, within A4249_RS07675 at 1529.809 kb on - strand, within A4249_RS07675 at 1529.809 kb on - strand, within A4249_RS07675 at 1529.809 kb on - strand, within A4249_RS07675 at 1529.856 kb on + strand, within A4249_RS07675 at 1529.856 kb on + strand, within A4249_RS07675 at 1529.856 kb on + strand, within A4249_RS07675 at 1529.857 kb on - strand, within A4249_RS07675 at 1529.884 kb on - strand, within A4249_RS07675 at 1529.925 kb on + strand, within A4249_RS07675 at 1529.925 kb on + strand, within A4249_RS07675 at 1529.925 kb on + strand, within A4249_RS07675 at 1529.926 kb on - strand, within A4249_RS07675 at 1529.926 kb on - strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.927 kb on + strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.928 kb on - strand, within A4249_RS07675 at 1529.934 kb on + strand, within A4249_RS07675 at 1529.934 kb on + strand, within A4249_RS07675 at 1529.934 kb on + strand, within A4249_RS07675 at 1529.934 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.985 kb on + strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.986 kb on - strand, within A4249_RS07675 at 1529.991 kb on + strand, within A4249_RS07675 at 1529.992 kb on - strand, within A4249_RS07675 at 1529.992 kb on - strand, within A4249_RS07675
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C) remove 1,527,133 + A4249_RS07660 0.88 -0.0 1,527,133 + A4249_RS07660 0.88 -0.8 1,527,155 + -1.5 1,527,155 + -0.1 1,527,156 - -1.3 1,527,156 - -0.0 1,527,156 - -0.6 1,527,156 - +0.4 1,527,156 - +0.2 1,527,156 - -0.6 1,527,156 - +0.4 1,527,156 - -0.7 1,527,156 - +0.6 1,527,194 + -0.5 1,527,195 - +0.3 1,527,206 + +0.9 1,527,206 + -0.2 1,527,206 + +0.3 1,527,207 - -0.3 1,527,207 - -0.4 1,527,726 + A4249_RS07665 0.62 -1.6 1,527,726 + A4249_RS07665 0.62 -1.2 1,527,727 - A4249_RS07665 0.62 -0.1 1,527,727 - A4249_RS07665 0.62 +2.2 1,527,966 + -0.2 1,527,966 + +0.0 1,527,967 - -0.1 1,527,967 - -0.7 1,528,025 + -0.1 1,528,210 + A4249_RS07670 0.16 +0.6 1,528,211 - A4249_RS07670 0.17 -0.9 1,528,320 + A4249_RS07670 0.27 -1.4 1,528,320 + A4249_RS07670 0.27 +0.0 1,528,320 + A4249_RS07670 0.27 -0.7 1,528,321 - A4249_RS07670 0.27 +1.2 1,528,365 + A4249_RS07670 0.31 +0.0 1,528,365 + A4249_RS07670 0.31 -0.1 1,528,365 + A4249_RS07670 0.31 +0.7 1,528,365 + A4249_RS07670 0.31 +0.0 1,528,366 - A4249_RS07670 0.31 +0.3 1,528,395 + A4249_RS07670 0.34 -0.4 1,528,395 + A4249_RS07670 0.34 -1.1 1,528,395 + A4249_RS07670 0.34 +0.7 1,528,396 - A4249_RS07670 0.34 -0.6 1,528,396 - A4249_RS07670 0.34 -0.7 1,528,545 + A4249_RS07670 0.49 -2.6 1,528,546 - A4249_RS07670 0.49 -0.7 1,528,546 - A4249_RS07670 0.49 +0.9 1,528,546 - A4249_RS07670 0.49 -1.2 1,528,570 + A4249_RS07670 0.51 +0.2 1,528,571 - A4249_RS07670 0.51 -0.6 1,528,571 - A4249_RS07670 0.51 +0.4 1,528,571 - A4249_RS07670 0.51 +0.1 1,528,784 + A4249_RS07670 0.71 -1.3 1,528,868 + A4249_RS07670 0.80 -0.1 1,528,868 + A4249_RS07670 0.80 -0.5 1,528,868 + A4249_RS07670 0.80 -2.3 1,528,868 + A4249_RS07670 0.80 +0.8 1,528,869 - A4249_RS07670 0.80 +0.4 1,528,869 - A4249_RS07670 0.80 +0.1 1,528,869 - A4249_RS07670 0.80 +0.0 1,529,038 + -0.1 1,529,038 + -0.1 1,529,039 - -0.1 1,529,039 - +0.4 1,529,204 + -0.4 1,529,205 - -0.3 1,529,415 + A4249_RS07675 0.11 -0.5 1,529,416 - A4249_RS07675 0.11 +0.1 1,529,416 - A4249_RS07675 0.11 -0.4 1,529,427 + A4249_RS07675 0.11 -0.3 1,529,427 + A4249_RS07675 0.11 -1.1 1,529,427 + A4249_RS07675 0.11 -1.7 1,529,427 + A4249_RS07675 0.11 -0.2 1,529,428 - A4249_RS07675 0.11 -0.1 1,529,428 - A4249_RS07675 0.11 +0.3 1,529,428 - A4249_RS07675 0.11 -0.2 1,529,428 - A4249_RS07675 0.11 +0.2 1,529,428 - A4249_RS07675 0.11 -0.1 1,529,428 - A4249_RS07675 0.11 +0.8 1,529,535 + A4249_RS07675 0.18 -0.2 1,529,535 + A4249_RS07675 0.18 +0.5 1,529,536 - A4249_RS07675 0.18 -0.8 1,529,536 - A4249_RS07675 0.18 -0.7 1,529,560 - A4249_RS07675 0.19 +0.4 1,529,562 + A4249_RS07675 0.20 -0.4 1,529,563 - A4249_RS07675 0.20 +0.2 1,529,563 - A4249_RS07675 0.20 -0.5 1,529,563 - A4249_RS07675 0.20 -0.2 1,529,563 - A4249_RS07675 0.20 +1.1 1,529,563 - A4249_RS07675 0.20 -0.2 1,529,628 + A4249_RS07675 0.24 +1.5 1,529,628 + A4249_RS07675 0.24 -0.1 1,529,628 + A4249_RS07675 0.24 +1.0 1,529,628 + A4249_RS07675 0.24 +0.3 1,529,628 + A4249_RS07675 0.24 -0.6 1,529,628 + A4249_RS07675 0.24 -0.8 1,529,628 + A4249_RS07675 0.24 +0.1 1,529,628 + A4249_RS07675 0.24 +0.0 1,529,629 - A4249_RS07675 0.24 +0.8 1,529,629 - A4249_RS07675 0.24 +0.0 1,529,629 - A4249_RS07675 0.24 -0.6 1,529,629 - A4249_RS07675 0.24 +0.6 1,529,629 - A4249_RS07675 0.24 -0.7 1,529,629 - A4249_RS07675 0.24 -0.4 1,529,629 - A4249_RS07675 0.24 +0.2 1,529,629 - A4249_RS07675 0.24 +1.6 1,529,670 + A4249_RS07675 0.26 -0.4 1,529,718 + A4249_RS07675 0.29 +0.4 1,529,718 + A4249_RS07675 0.29 +0.4 1,529,718 + A4249_RS07675 0.29 -1.8 1,529,718 + A4249_RS07675 0.29 +0.2 1,529,718 + A4249_RS07675 0.29 -0.8 1,529,718 + A4249_RS07675 0.29 -0.1 1,529,718 + A4249_RS07675 0.29 -0.8 1,529,718 + A4249_RS07675 0.29 +0.0 1,529,719 - A4249_RS07675 0.29 -0.4 1,529,719 - A4249_RS07675 0.29 +0.4 1,529,721 + A4249_RS07675 0.29 -0.0 1,529,721 + A4249_RS07675 0.29 +0.3 1,529,722 - A4249_RS07675 0.29 +1.1 1,529,784 + A4249_RS07675 0.33 +0.6 1,529,784 + A4249_RS07675 0.33 -0.4 1,529,784 + A4249_RS07675 0.33 +0.1 1,529,785 - A4249_RS07675 0.33 +0.9 1,529,785 - A4249_RS07675 0.33 +0.7 1,529,785 - A4249_RS07675 0.33 +0.4 1,529,785 - A4249_RS07675 0.33 +0.2 1,529,785 - A4249_RS07675 0.33 -0.3 1,529,785 - A4249_RS07675 0.33 +0.1 1,529,808 + A4249_RS07675 0.34 -0.2 1,529,808 + A4249_RS07675 0.34 +0.9 1,529,808 + A4249_RS07675 0.34 +0.0 1,529,809 - A4249_RS07675 0.35 -1.5 1,529,809 - A4249_RS07675 0.35 +0.2 1,529,809 - A4249_RS07675 0.35 -0.8 1,529,856 + A4249_RS07675 0.37 +1.1 1,529,856 + A4249_RS07675 0.37 -0.7 1,529,856 + A4249_RS07675 0.37 +0.6 1,529,857 - A4249_RS07675 0.37 +0.1 1,529,884 - A4249_RS07675 0.39 -0.9 1,529,925 + A4249_RS07675 0.42 +0.5 1,529,925 + A4249_RS07675 0.42 +0.2 1,529,925 + A4249_RS07675 0.42 -2.2 1,529,926 - A4249_RS07675 0.42 +0.8 1,529,926 - A4249_RS07675 0.42 -0.3 1,529,927 + A4249_RS07675 0.42 +0.1 1,529,927 + A4249_RS07675 0.42 +0.1 1,529,927 + A4249_RS07675 0.42 +0.0 1,529,927 + A4249_RS07675 0.42 -0.3 1,529,927 + A4249_RS07675 0.42 +0.2 1,529,927 + A4249_RS07675 0.42 -0.7 1,529,928 - A4249_RS07675 0.42 +0.4 1,529,928 - A4249_RS07675 0.42 +0.1 1,529,928 - A4249_RS07675 0.42 -1.0 1,529,928 - A4249_RS07675 0.42 +0.7 1,529,928 - A4249_RS07675 0.42 +0.7 1,529,928 - A4249_RS07675 0.42 -0.7 1,529,928 - A4249_RS07675 0.42 -0.2 1,529,928 - A4249_RS07675 0.42 -0.3 1,529,928 - A4249_RS07675 0.42 -0.2 1,529,928 - A4249_RS07675 0.42 +0.8 1,529,928 - A4249_RS07675 0.42 +0.4 1,529,934 + A4249_RS07675 0.42 +0.6 1,529,934 + A4249_RS07675 0.42 -0.6 1,529,934 + A4249_RS07675 0.42 +0.9 1,529,934 + A4249_RS07675 0.42 +0.4 1,529,985 + A4249_RS07675 0.45 +0.2 1,529,985 + A4249_RS07675 0.45 -1.0 1,529,985 + A4249_RS07675 0.45 +0.2 1,529,985 + A4249_RS07675 0.45 -1.1 1,529,985 + A4249_RS07675 0.45 -0.1 1,529,985 + A4249_RS07675 0.45 +0.5 1,529,985 + A4249_RS07675 0.45 +0.8 1,529,985 + A4249_RS07675 0.45 -0.8 1,529,985 + A4249_RS07675 0.45 +0.9 1,529,985 + A4249_RS07675 0.45 +0.1 1,529,985 + A4249_RS07675 0.45 +0.1 1,529,985 + A4249_RS07675 0.45 -0.2 1,529,986 - A4249_RS07675 0.45 -0.0 1,529,986 - A4249_RS07675 0.45 -0.2 1,529,986 - A4249_RS07675 0.45 +0.2 1,529,986 - A4249_RS07675 0.45 +0.2 1,529,986 - A4249_RS07675 0.45 -0.2 1,529,986 - A4249_RS07675 0.45 -0.8 1,529,986 - A4249_RS07675 0.45 -1.0 1,529,986 - A4249_RS07675 0.45 -1.2 1,529,986 - A4249_RS07675 0.45 +0.7 1,529,986 - A4249_RS07675 0.45 +0.9 1,529,986 - A4249_RS07675 0.45 +1.6 1,529,991 + A4249_RS07675 0.46 +0.6 1,529,992 - A4249_RS07675 0.46 -0.3 1,529,992 - A4249_RS07675 0.46 +1.0
Or see this region's nucleotide sequence