Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01610 and A4249_RS01615 are separated by 46 nucleotides A4249_RS01615 and A4249_RS01620 overlap by 4 nucleotides A4249_RS01620 and A4249_RS01625 are separated by 171 nucleotides
A4249_RS01610: A4249_RS01610 - TetR/AcrR family transcriptional regulator, at 305,423 to 305,998
_RS01610
A4249_RS01615: A4249_RS01615 - GNAT family N-acetyltransferase, at 306,045 to 306,554
_RS01615
A4249_RS01620: A4249_RS01620 - S46 family peptidase, at 306,551 to 308,629
_RS01620
A4249_RS01625: A4249_RS01625 - S46 family peptidase, at 308,801 to 310,894
_RS01625
Position (kb) 306
307
308
309 Strain fitness (log2 ratio) -2
-1
0
1
2 at 305.844 kb on + strand, within A4249_RS01610 at 305.844 kb on + strand, within A4249_RS01610 at 306.046 kb on + strand at 306.046 kb on + strand at 306.047 kb on - strand at 306.145 kb on + strand, within A4249_RS01615 at 306.146 kb on - strand, within A4249_RS01615 at 306.146 kb on - strand, within A4249_RS01615 at 306.298 kb on + strand, within A4249_RS01615 at 306.298 kb on + strand, within A4249_RS01615 at 306.298 kb on + strand, within A4249_RS01615 at 306.482 kb on - strand, within A4249_RS01615 at 306.594 kb on + strand at 306.594 kb on + strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.663 kb on + strand at 306.663 kb on + strand at 306.664 kb on - strand at 306.669 kb on + strand at 306.669 kb on + strand at 306.670 kb on - strand at 306.670 kb on - strand at 306.670 kb on - strand at 306.670 kb on - strand at 306.670 kb on - strand at 306.942 kb on + strand, within A4249_RS01620 at 306.942 kb on + strand, within A4249_RS01620 at 306.942 kb on + strand, within A4249_RS01620 at 306.942 kb on + strand, within A4249_RS01620 at 306.943 kb on - strand, within A4249_RS01620 at 306.943 kb on - strand, within A4249_RS01620 at 306.943 kb on - strand, within A4249_RS01620 at 306.943 kb on - strand, within A4249_RS01620 at 306.943 kb on - strand, within A4249_RS01620 at 306.990 kb on + strand, within A4249_RS01620 at 306.990 kb on + strand, within A4249_RS01620 at 307.051 kb on + strand, within A4249_RS01620 at 307.051 kb on + strand, within A4249_RS01620 at 307.051 kb on + strand, within A4249_RS01620 at 307.051 kb on + strand, within A4249_RS01620 at 307.051 kb on + strand, within A4249_RS01620 at 307.052 kb on - strand, within A4249_RS01620 at 307.052 kb on - strand, within A4249_RS01620 at 307.104 kb on + strand, within A4249_RS01620 at 307.104 kb on + strand, within A4249_RS01620 at 307.105 kb on - strand, within A4249_RS01620 at 307.105 kb on - strand, within A4249_RS01620 at 307.105 kb on - strand, within A4249_RS01620 at 307.105 kb on - strand, within A4249_RS01620 at 307.105 kb on - strand, within A4249_RS01620 at 307.254 kb on + strand, within A4249_RS01620 at 307.254 kb on + strand, within A4249_RS01620 at 307.530 kb on + strand, within A4249_RS01620 at 307.530 kb on + strand, within A4249_RS01620 at 307.530 kb on + strand, within A4249_RS01620 at 307.531 kb on - strand, within A4249_RS01620 at 307.531 kb on - strand, within A4249_RS01620 at 307.531 kb on - strand, within A4249_RS01620 at 307.605 kb on + strand, within A4249_RS01620 at 307.605 kb on + strand, within A4249_RS01620 at 307.605 kb on + strand, within A4249_RS01620 at 307.605 kb on + strand, within A4249_RS01620 at 307.606 kb on - strand, within A4249_RS01620 at 307.606 kb on - strand, within A4249_RS01620 at 307.676 kb on - strand, within A4249_RS01620 at 307.676 kb on - strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.695 kb on + strand, within A4249_RS01620 at 307.696 kb on - strand, within A4249_RS01620 at 307.696 kb on - strand, within A4249_RS01620 at 307.762 kb on - strand, within A4249_RS01620 at 307.965 kb on + strand, within A4249_RS01620 at 307.965 kb on + strand, within A4249_RS01620 at 307.965 kb on + strand, within A4249_RS01620 at 307.966 kb on - strand, within A4249_RS01620 at 307.966 kb on - strand, within A4249_RS01620 at 307.966 kb on - strand, within A4249_RS01620 at 308.079 kb on + strand, within A4249_RS01620 at 308.079 kb on + strand, within A4249_RS01620 at 308.080 kb on - strand, within A4249_RS01620 at 308.094 kb on + strand, within A4249_RS01620 at 308.094 kb on + strand, within A4249_RS01620 at 308.309 kb on + strand, within A4249_RS01620 at 308.329 kb on + strand, within A4249_RS01620 at 308.329 kb on + strand, within A4249_RS01620 at 308.329 kb on + strand, within A4249_RS01620 at 308.346 kb on + strand, within A4249_RS01620 at 308.346 kb on + strand, within A4249_RS01620 at 308.347 kb on - strand, within A4249_RS01620 at 308.347 kb on - strand, within A4249_RS01620 at 308.347 kb on - strand, within A4249_RS01620 at 308.497 kb on + strand at 308.498 kb on - strand at 308.498 kb on - strand at 308.544 kb on + strand at 308.544 kb on + strand at 308.544 kb on + strand at 308.545 kb on - strand at 308.545 kb on - strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.844 kb on + strand at 308.845 kb on - strand at 308.845 kb on - strand at 308.845 kb on - strand at 308.845 kb on - strand at 308.845 kb on - strand at 308.845 kb on - strand at 309.039 kb on + strand, within A4249_RS01625 at 309.039 kb on + strand, within A4249_RS01625 at 309.039 kb on + strand, within A4249_RS01625 at 309.039 kb on + strand, within A4249_RS01625 at 309.039 kb on + strand, within A4249_RS01625 at 309.040 kb on - strand, within A4249_RS01625 at 309.040 kb on - strand, within A4249_RS01625 at 309.040 kb on - strand, within A4249_RS01625 at 309.040 kb on - strand, within A4249_RS01625 at 309.040 kb on - strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.123 kb on + strand, within A4249_RS01625 at 309.124 kb on - strand, within A4249_RS01625 at 309.124 kb on - strand, within A4249_RS01625 at 309.168 kb on + strand, within A4249_RS01625 at 309.168 kb on + strand, within A4249_RS01625 at 309.168 kb on + strand, within A4249_RS01625 at 309.169 kb on - strand, within A4249_RS01625 at 309.169 kb on - strand, within A4249_RS01625 at 309.192 kb on + strand, within A4249_RS01625 at 309.193 kb on - strand, within A4249_RS01625 at 309.225 kb on + strand, within A4249_RS01625 at 309.226 kb on - strand, within A4249_RS01625 at 309.240 kb on + strand, within A4249_RS01625 at 309.240 kb on + strand, within A4249_RS01625 at 309.525 kb on + strand, within A4249_RS01625 at 309.525 kb on + strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.526 kb on - strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.558 kb on + strand, within A4249_RS01625 at 309.559 kb on - strand, within A4249_RS01625 at 309.559 kb on - strand, within A4249_RS01625 at 309.559 kb on - strand, within A4249_RS01625 at 309.559 kb on - strand, within A4249_RS01625 at 309.560 kb on + strand, within A4249_RS01625
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C) remove 305,844 + A4249_RS01610 0.73 -1.3 305,844 + A4249_RS01610 0.73 +0.7 306,046 + -0.5 306,046 + -0.6 306,047 - -1.6 306,145 + A4249_RS01615 0.20 +0.3 306,146 - A4249_RS01615 0.20 +0.2 306,146 - A4249_RS01615 0.20 +0.7 306,298 + A4249_RS01615 0.50 -0.6 306,298 + A4249_RS01615 0.50 +0.2 306,298 + A4249_RS01615 0.50 -1.1 306,482 - A4249_RS01615 0.86 +0.4 306,594 + -0.3 306,594 + +1.3 306,595 - +0.5 306,595 - -1.7 306,595 - +0.4 306,595 - -1.1 306,595 - +0.8 306,595 - +0.7 306,595 - -0.1 306,663 + -0.2 306,663 + +0.1 306,664 - +1.2 306,669 + -0.3 306,669 + -0.1 306,670 - -0.4 306,670 - +0.5 306,670 - -1.3 306,670 - +0.2 306,670 - -0.3 306,942 + A4249_RS01620 0.19 +1.1 306,942 + A4249_RS01620 0.19 -0.1 306,942 + A4249_RS01620 0.19 -0.8 306,942 + A4249_RS01620 0.19 -0.3 306,943 - A4249_RS01620 0.19 +0.6 306,943 - A4249_RS01620 0.19 -1.4 306,943 - A4249_RS01620 0.19 +0.2 306,943 - A4249_RS01620 0.19 +1.0 306,943 - A4249_RS01620 0.19 +0.4 306,990 + A4249_RS01620 0.21 -0.2 306,990 + A4249_RS01620 0.21 +0.0 307,051 + A4249_RS01620 0.24 -0.1 307,051 + A4249_RS01620 0.24 -0.5 307,051 + A4249_RS01620 0.24 -0.5 307,051 + A4249_RS01620 0.24 -0.2 307,051 + A4249_RS01620 0.24 -0.3 307,052 - A4249_RS01620 0.24 -1.1 307,052 - A4249_RS01620 0.24 +0.2 307,104 + A4249_RS01620 0.27 -1.3 307,104 + A4249_RS01620 0.27 +0.4 307,105 - A4249_RS01620 0.27 +0.6 307,105 - A4249_RS01620 0.27 +0.2 307,105 - A4249_RS01620 0.27 -1.4 307,105 - A4249_RS01620 0.27 -0.7 307,105 - A4249_RS01620 0.27 -0.1 307,254 + A4249_RS01620 0.34 -1.1 307,254 + A4249_RS01620 0.34 +0.1 307,530 + A4249_RS01620 0.47 +0.4 307,530 + A4249_RS01620 0.47 -0.6 307,530 + A4249_RS01620 0.47 -0.3 307,531 - A4249_RS01620 0.47 -0.6 307,531 - A4249_RS01620 0.47 -0.1 307,531 - A4249_RS01620 0.47 -0.3 307,605 + A4249_RS01620 0.51 +1.1 307,605 + A4249_RS01620 0.51 +0.1 307,605 + A4249_RS01620 0.51 +0.5 307,605 + A4249_RS01620 0.51 +1.0 307,606 - A4249_RS01620 0.51 -0.5 307,606 - A4249_RS01620 0.51 -0.2 307,676 - A4249_RS01620 0.54 -0.3 307,676 - A4249_RS01620 0.54 -0.1 307,695 + A4249_RS01620 0.55 +1.4 307,695 + A4249_RS01620 0.55 -0.2 307,695 + A4249_RS01620 0.55 -0.4 307,695 + A4249_RS01620 0.55 +1.3 307,695 + A4249_RS01620 0.55 +0.1 307,695 + A4249_RS01620 0.55 -0.1 307,695 + A4249_RS01620 0.55 +1.3 307,695 + A4249_RS01620 0.55 +0.7 307,695 + A4249_RS01620 0.55 -1.2 307,695 + A4249_RS01620 0.55 +0.1 307,695 + A4249_RS01620 0.55 +0.3 307,695 + A4249_RS01620 0.55 -1.1 307,696 - A4249_RS01620 0.55 -0.8 307,696 - A4249_RS01620 0.55 +0.1 307,762 - A4249_RS01620 0.58 +0.5 307,965 + A4249_RS01620 0.68 +0.2 307,965 + A4249_RS01620 0.68 +0.5 307,965 + A4249_RS01620 0.68 -0.6 307,966 - A4249_RS01620 0.68 +1.4 307,966 - A4249_RS01620 0.68 +0.4 307,966 - A4249_RS01620 0.68 -0.0 308,079 + A4249_RS01620 0.73 +0.0 308,079 + A4249_RS01620 0.73 -0.3 308,080 - A4249_RS01620 0.74 -1.4 308,094 + A4249_RS01620 0.74 -0.4 308,094 + A4249_RS01620 0.74 -0.5 308,309 + A4249_RS01620 0.85 -0.1 308,329 + A4249_RS01620 0.86 +0.5 308,329 + A4249_RS01620 0.86 +0.4 308,329 + A4249_RS01620 0.86 +1.4 308,346 + A4249_RS01620 0.86 -0.6 308,346 + A4249_RS01620 0.86 -0.8 308,347 - A4249_RS01620 0.86 -1.5 308,347 - A4249_RS01620 0.86 -0.0 308,347 - A4249_RS01620 0.86 -0.7 308,497 + -0.6 308,498 - +0.2 308,498 - -0.0 308,544 + +0.1 308,544 + -0.5 308,544 + +0.3 308,545 - +0.5 308,545 - +0.2 308,844 + +0.4 308,844 + -0.1 308,844 + +0.1 308,844 + +0.3 308,844 + -0.4 308,844 + -0.6 308,844 + -1.1 308,845 - -0.8 308,845 - -0.8 308,845 - +0.3 308,845 - +1.4 308,845 - +0.8 308,845 - +1.0 309,039 + A4249_RS01625 0.11 -0.2 309,039 + A4249_RS01625 0.11 +0.1 309,039 + A4249_RS01625 0.11 -0.5 309,039 + A4249_RS01625 0.11 +0.8 309,039 + A4249_RS01625 0.11 -1.3 309,040 - A4249_RS01625 0.11 -0.3 309,040 - A4249_RS01625 0.11 +1.3 309,040 - A4249_RS01625 0.11 -1.3 309,040 - A4249_RS01625 0.11 -0.8 309,040 - A4249_RS01625 0.11 +0.7 309,123 + A4249_RS01625 0.15 -1.5 309,123 + A4249_RS01625 0.15 +1.9 309,123 + A4249_RS01625 0.15 +0.5 309,123 + A4249_RS01625 0.15 +0.2 309,123 + A4249_RS01625 0.15 -0.1 309,123 + A4249_RS01625 0.15 +0.9 309,124 - A4249_RS01625 0.15 -0.4 309,124 - A4249_RS01625 0.15 -0.6 309,168 + A4249_RS01625 0.18 -1.9 309,168 + A4249_RS01625 0.18 +0.9 309,168 + A4249_RS01625 0.18 +2.3 309,169 - A4249_RS01625 0.18 +0.5 309,169 - A4249_RS01625 0.18 +0.1 309,192 + A4249_RS01625 0.19 -1.0 309,193 - A4249_RS01625 0.19 -1.2 309,225 + A4249_RS01625 0.20 -1.2 309,226 - A4249_RS01625 0.20 -0.7 309,240 + A4249_RS01625 0.21 -0.5 309,240 + A4249_RS01625 0.21 +0.3 309,525 + A4249_RS01625 0.35 -0.7 309,525 + A4249_RS01625 0.35 -0.0 309,526 - A4249_RS01625 0.35 -0.2 309,526 - A4249_RS01625 0.35 -0.4 309,526 - A4249_RS01625 0.35 -0.5 309,526 - A4249_RS01625 0.35 -0.8 309,526 - A4249_RS01625 0.35 -0.1 309,526 - A4249_RS01625 0.35 +1.5 309,558 + A4249_RS01625 0.36 +2.6 309,558 + A4249_RS01625 0.36 +0.5 309,558 + A4249_RS01625 0.36 +0.9 309,558 + A4249_RS01625 0.36 -0.4 309,558 + A4249_RS01625 0.36 -0.6 309,558 + A4249_RS01625 0.36 +0.5 309,558 + A4249_RS01625 0.36 +0.6 309,558 + A4249_RS01625 0.36 -0.4 309,559 - A4249_RS01625 0.36 -0.5 309,559 - A4249_RS01625 0.36 +1.6 309,559 - A4249_RS01625 0.36 +1.0 309,559 - A4249_RS01625 0.36 +1.5 309,560 + A4249_RS01625 0.36 +0.4
Or see this region's nucleotide sequence