Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS01590 and A4249_RS01595 are separated by 193 nucleotides A4249_RS01595 and A4249_RS16715 are separated by 7 nucleotides A4249_RS16715 and A4249_RS01600 are separated by 50 nucleotides A4249_RS01600 and A4249_RS01605 are separated by 13 nucleotides
A4249_RS01590: A4249_RS01590 - site-specific DNA-methyltransferase, at 300,874 to 301,965
_RS01590
A4249_RS01595: A4249_RS01595 - hypothetical protein, at 302,159 to 302,362
_RS01595
A4249_RS16715: A4249_RS16715 - hypothetical protein, at 302,370 to 302,546
_RS16715
A4249_RS01600: A4249_RS01600 - HlyD family secretion protein, at 302,597 to 303,712
_RS01600
A4249_RS01605: A4249_RS01605 - MFS transporter, at 303,726 to 305,423
_RS01605
Position (kb)
302
303
304 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 301.752 kb on - strand, within A4249_RS01590 at 301.847 kb on + strand, within A4249_RS01590 at 302.016 kb on - strand at 302.016 kb on - strand at 302.026 kb on - strand at 302.120 kb on + strand at 302.120 kb on + strand at 302.120 kb on + strand at 302.120 kb on + strand at 302.120 kb on + strand at 302.120 kb on + strand at 302.121 kb on - strand at 302.121 kb on - strand at 302.121 kb on - strand at 302.121 kb on - strand at 302.121 kb on - strand at 302.121 kb on - strand at 302.182 kb on + strand, within A4249_RS01595 at 302.182 kb on + strand, within A4249_RS01595 at 302.182 kb on + strand, within A4249_RS01595 at 302.182 kb on + strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.183 kb on - strand, within A4249_RS01595 at 302.248 kb on + strand, within A4249_RS01595 at 302.248 kb on + strand, within A4249_RS01595 at 302.248 kb on + strand, within A4249_RS01595 at 302.248 kb on + strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.249 kb on - strand, within A4249_RS01595 at 302.558 kb on + strand at 302.559 kb on - strand at 302.559 kb on - strand at 302.570 kb on + strand at 302.570 kb on + strand at 302.571 kb on - strand at 302.608 kb on + strand at 302.608 kb on + strand at 302.609 kb on - strand at 302.609 kb on - strand at 302.872 kb on + strand, within A4249_RS01600 at 302.872 kb on + strand, within A4249_RS01600 at 302.872 kb on + strand, within A4249_RS01600 at 302.873 kb on - strand, within A4249_RS01600 at 302.873 kb on - strand, within A4249_RS01600 at 302.873 kb on - strand, within A4249_RS01600 at 302.873 kb on - strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.934 kb on + strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 302.935 kb on - strand, within A4249_RS01600 at 303.381 kb on + strand, within A4249_RS01600 at 303.382 kb on - strand, within A4249_RS01600 at 303.382 kb on - strand, within A4249_RS01600 at 303.382 kb on - strand, within A4249_RS01600 at 303.459 kb on + strand, within A4249_RS01600 at 303.459 kb on + strand, within A4249_RS01600 at 303.459 kb on + strand, within A4249_RS01600 at 303.459 kb on + strand, within A4249_RS01600 at 303.460 kb on - strand, within A4249_RS01600 at 303.460 kb on - strand, within A4249_RS01600 at 303.460 kb on - strand, within A4249_RS01600 at 303.460 kb on - strand, within A4249_RS01600 at 303.504 kb on + strand, within A4249_RS01600 at 303.504 kb on + strand, within A4249_RS01600 at 303.504 kb on + strand, within A4249_RS01600 at 303.505 kb on - strand, within A4249_RS01600 at 303.505 kb on - strand, within A4249_RS01600 at 303.505 kb on - strand, within A4249_RS01600 at 303.505 kb on - strand, within A4249_RS01600 at 303.505 kb on - strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.558 kb on + strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.559 kb on - strand, within A4249_RS01600 at 303.976 kb on + strand, within A4249_RS01605 at 303.977 kb on - strand, within A4249_RS01605 at 304.058 kb on - strand, within A4249_RS01605 at 304.058 kb on - strand, within A4249_RS01605 at 304.058 kb on - strand, within A4249_RS01605 at 304.058 kb on - strand, within A4249_RS01605 at 304.058 kb on - strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.252 kb on + strand, within A4249_RS01605 at 304.253 kb on - strand, within A4249_RS01605 at 304.253 kb on - strand, within A4249_RS01605 at 304.253 kb on - strand, within A4249_RS01605 at 304.545 kb on - strand, within A4249_RS01605
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C) remove 301,752 - A4249_RS01590 0.80 -0.1 301,847 + A4249_RS01590 0.89 +0.9 302,016 - +0.9 302,016 - +2.6 302,026 - +1.2 302,120 + -0.0 302,120 + -0.4 302,120 + +0.6 302,120 + +0.8 302,120 + -0.1 302,120 + -0.5 302,121 - +0.9 302,121 - +0.1 302,121 - +0.4 302,121 - -0.2 302,121 - -0.6 302,121 - +0.5 302,182 + A4249_RS01595 0.11 +1.3 302,182 + A4249_RS01595 0.11 -0.5 302,182 + A4249_RS01595 0.11 +0.2 302,182 + A4249_RS01595 0.11 +0.6 302,183 - A4249_RS01595 0.12 +0.3 302,183 - A4249_RS01595 0.12 -0.2 302,183 - A4249_RS01595 0.12 -0.9 302,183 - A4249_RS01595 0.12 +0.2 302,183 - A4249_RS01595 0.12 +0.5 302,183 - A4249_RS01595 0.12 -0.3 302,183 - A4249_RS01595 0.12 -0.3 302,183 - A4249_RS01595 0.12 +0.7 302,183 - A4249_RS01595 0.12 -0.3 302,248 + A4249_RS01595 0.44 +0.7 302,248 + A4249_RS01595 0.44 -0.8 302,248 + A4249_RS01595 0.44 -0.4 302,248 + A4249_RS01595 0.44 -0.4 302,249 - A4249_RS01595 0.44 -1.0 302,249 - A4249_RS01595 0.44 -0.8 302,249 - A4249_RS01595 0.44 -0.6 302,249 - A4249_RS01595 0.44 -0.0 302,249 - A4249_RS01595 0.44 -0.6 302,249 - A4249_RS01595 0.44 +0.1 302,249 - A4249_RS01595 0.44 +0.6 302,558 + +0.1 302,559 - +0.3 302,559 - -0.5 302,570 + -0.0 302,570 + -0.7 302,571 - -0.1 302,608 + +0.3 302,608 + +0.3 302,609 - -0.2 302,609 - +0.0 302,872 + A4249_RS01600 0.25 -1.9 302,872 + A4249_RS01600 0.25 -0.2 302,872 + A4249_RS01600 0.25 +1.1 302,873 - A4249_RS01600 0.25 +0.6 302,873 - A4249_RS01600 0.25 -0.4 302,873 - A4249_RS01600 0.25 +0.1 302,873 - A4249_RS01600 0.25 +1.0 302,934 + A4249_RS01600 0.30 -0.6 302,934 + A4249_RS01600 0.30 +1.5 302,934 + A4249_RS01600 0.30 -0.8 302,934 + A4249_RS01600 0.30 -0.5 302,934 + A4249_RS01600 0.30 -1.0 302,934 + A4249_RS01600 0.30 -0.9 302,934 + A4249_RS01600 0.30 -0.4 302,934 + A4249_RS01600 0.30 -0.1 302,934 + A4249_RS01600 0.30 +0.6 302,935 - A4249_RS01600 0.30 -0.6 302,935 - A4249_RS01600 0.30 +0.6 302,935 - A4249_RS01600 0.30 -1.3 302,935 - A4249_RS01600 0.30 +1.3 302,935 - A4249_RS01600 0.30 -0.5 302,935 - A4249_RS01600 0.30 +0.4 302,935 - A4249_RS01600 0.30 +0.2 302,935 - A4249_RS01600 0.30 -0.7 302,935 - A4249_RS01600 0.30 +0.4 302,935 - A4249_RS01600 0.30 -0.1 303,381 + A4249_RS01600 0.70 +0.3 303,382 - A4249_RS01600 0.70 -0.1 303,382 - A4249_RS01600 0.70 +0.1 303,382 - A4249_RS01600 0.70 +0.7 303,459 + A4249_RS01600 0.77 -0.4 303,459 + A4249_RS01600 0.77 -0.4 303,459 + A4249_RS01600 0.77 -0.7 303,459 + A4249_RS01600 0.77 +0.1 303,460 - A4249_RS01600 0.77 +0.2 303,460 - A4249_RS01600 0.77 +0.6 303,460 - A4249_RS01600 0.77 +0.6 303,460 - A4249_RS01600 0.77 +0.6 303,504 + A4249_RS01600 0.81 +0.1 303,504 + A4249_RS01600 0.81 -0.5 303,504 + A4249_RS01600 0.81 -0.3 303,505 - A4249_RS01600 0.81 -1.4 303,505 - A4249_RS01600 0.81 -0.1 303,505 - A4249_RS01600 0.81 -0.8 303,505 - A4249_RS01600 0.81 +0.4 303,505 - A4249_RS01600 0.81 -1.6 303,558 + A4249_RS01600 0.86 -0.1 303,558 + A4249_RS01600 0.86 +0.5 303,558 + A4249_RS01600 0.86 -0.8 303,558 + A4249_RS01600 0.86 -0.1 303,558 + A4249_RS01600 0.86 +0.0 303,558 + A4249_RS01600 0.86 +1.4 303,559 - A4249_RS01600 0.86 -0.1 303,559 - A4249_RS01600 0.86 -0.9 303,559 - A4249_RS01600 0.86 -0.6 303,559 - A4249_RS01600 0.86 +0.7 303,559 - A4249_RS01600 0.86 +1.1 303,559 - A4249_RS01600 0.86 -0.3 303,559 - A4249_RS01600 0.86 -0.9 303,559 - A4249_RS01600 0.86 -0.5 303,559 - A4249_RS01600 0.86 -0.8 303,976 + A4249_RS01605 0.15 -1.0 303,977 - A4249_RS01605 0.15 -0.1 304,058 - A4249_RS01605 0.20 +0.6 304,058 - A4249_RS01605 0.20 -0.6 304,058 - A4249_RS01605 0.20 +0.5 304,058 - A4249_RS01605 0.20 -0.9 304,058 - A4249_RS01605 0.20 -0.6 304,252 + A4249_RS01605 0.31 +1.4 304,252 + A4249_RS01605 0.31 +0.2 304,252 + A4249_RS01605 0.31 +0.9 304,252 + A4249_RS01605 0.31 -0.4 304,252 + A4249_RS01605 0.31 -1.1 304,252 + A4249_RS01605 0.31 -0.4 304,253 - A4249_RS01605 0.31 +0.2 304,253 - A4249_RS01605 0.31 +0.3 304,253 - A4249_RS01605 0.31 +1.1 304,545 - A4249_RS01605 0.48 -2.1
Or see this region's nucleotide sequence