Experiment: HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00410 and A4249_RS00415 are separated by 61 nucleotides A4249_RS00415 and A4249_RS00420 are separated by 63 nucleotides A4249_RS00420 and A4249_RS00425 are separated by 61 nucleotides A4249_RS00425 and A4249_RS00430 are separated by 6 nucleotides
A4249_RS00410: A4249_RS00410 - OmpA family protein, at 69,764 to 70,315
_RS00410
A4249_RS00415: A4249_RS00415 - NAD-dependent deacylase, at 70,377 to 71,087
_RS00415
A4249_RS00420: A4249_RS00420 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 71,151 to 72,257
_RS00420
A4249_RS00425: A4249_RS00425 - VOC family protein, at 72,319 to 72,702
_RS00425
A4249_RS00430: A4249_RS00430 - glutathione S-transferase family protein, at 72,709 to 73,395
_RS00430
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 70.229 kb on + strand, within A4249_RS00410 at 70.229 kb on + strand, within A4249_RS00410 at 70.229 kb on + strand, within A4249_RS00410 at 70.230 kb on - strand, within A4249_RS00410 at 70.230 kb on - strand, within A4249_RS00410 at 70.314 kb on - strand at 70.314 kb on - strand at 70.378 kb on + strand at 70.379 kb on - strand at 70.379 kb on - strand at 70.495 kb on + strand, within A4249_RS00415 at 70.748 kb on + strand, within A4249_RS00415 at 70.748 kb on + strand, within A4249_RS00415 at 70.749 kb on - strand, within A4249_RS00415 at 70.749 kb on - strand, within A4249_RS00415 at 70.749 kb on - strand, within A4249_RS00415 at 70.749 kb on - strand, within A4249_RS00415 at 70.922 kb on + strand, within A4249_RS00415 at 70.922 kb on + strand, within A4249_RS00415 at 70.922 kb on + strand, within A4249_RS00415 at 70.922 kb on + strand, within A4249_RS00415 at 70.923 kb on - strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.924 kb on + strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.925 kb on - strand, within A4249_RS00415 at 70.958 kb on - strand, within A4249_RS00415 at 70.958 kb on - strand, within A4249_RS00415 at 71.047 kb on - strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.098 kb on + strand at 71.099 kb on - strand at 71.099 kb on - strand at 71.099 kb on - strand at 71.099 kb on - strand at 71.099 kb on - strand at 71.099 kb on - strand at 71.117 kb on + strand at 71.117 kb on + strand at 71.117 kb on + strand at 71.117 kb on + strand at 71.117 kb on + strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.118 kb on - strand at 71.283 kb on + strand, within A4249_RS00420 at 71.283 kb on + strand, within A4249_RS00420 at 71.284 kb on - strand, within A4249_RS00420 at 71.284 kb on - strand, within A4249_RS00420 at 71.284 kb on - strand, within A4249_RS00420 at 71.284 kb on - strand, within A4249_RS00420 at 71.284 kb on - strand, within A4249_RS00420 at 71.285 kb on + strand, within A4249_RS00420 at 71.285 kb on + strand, within A4249_RS00420 at 71.285 kb on + strand, within A4249_RS00420 at 71.286 kb on - strand, within A4249_RS00420 at 71.286 kb on - strand, within A4249_RS00420 at 71.286 kb on - strand, within A4249_RS00420 at 71.330 kb on - strand, within A4249_RS00420 at 71.412 kb on + strand, within A4249_RS00420 at 71.413 kb on - strand, within A4249_RS00420 at 71.413 kb on - strand, within A4249_RS00420 at 71.435 kb on + strand, within A4249_RS00420 at 71.435 kb on + strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.436 kb on - strand, within A4249_RS00420 at 71.529 kb on + strand, within A4249_RS00420 at 71.529 kb on + strand, within A4249_RS00420 at 71.529 kb on + strand, within A4249_RS00420 at 71.529 kb on + strand, within A4249_RS00420 at 71.529 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.553 kb on + strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.554 kb on - strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.555 kb on + strand, within A4249_RS00420 at 71.556 kb on - strand, within A4249_RS00420 at 71.556 kb on - strand, within A4249_RS00420 at 71.556 kb on - strand, within A4249_RS00420 at 71.646 kb on + strand, within A4249_RS00420 at 71.646 kb on + strand, within A4249_RS00420 at 71.646 kb on + strand, within A4249_RS00420 at 71.646 kb on + strand, within A4249_RS00420 at 71.646 kb on + strand, within A4249_RS00420 at 71.647 kb on - strand, within A4249_RS00420 at 71.647 kb on - strand, within A4249_RS00420 at 71.647 kb on - strand, within A4249_RS00420 at 71.922 kb on + strand, within A4249_RS00420 at 71.922 kb on + strand, within A4249_RS00420 at 71.922 kb on + strand, within A4249_RS00420 at 71.922 kb on + strand, within A4249_RS00420 at 71.922 kb on + strand, within A4249_RS00420 at 71.923 kb on - strand, within A4249_RS00420 at 71.923 kb on - strand, within A4249_RS00420 at 71.923 kb on - strand, within A4249_RS00420 at 71.923 kb on - strand, within A4249_RS00420 at 72.135 kb on + strand, within A4249_RS00420 at 72.135 kb on + strand, within A4249_RS00420 at 72.135 kb on + strand, within A4249_RS00420 at 72.135 kb on + strand, within A4249_RS00420 at 72.135 kb on + strand, within A4249_RS00420 at 72.136 kb on - strand, within A4249_RS00420 at 72.136 kb on - strand, within A4249_RS00420 at 72.136 kb on - strand, within A4249_RS00420 at 72.136 kb on - strand, within A4249_RS00420 at 72.374 kb on - strand, within A4249_RS00425 at 72.374 kb on - strand, within A4249_RS00425 at 72.374 kb on - strand, within A4249_RS00425 at 72.649 kb on + strand, within A4249_RS00425 at 72.649 kb on + strand, within A4249_RS00425 at 72.650 kb on - strand, within A4249_RS00425 at 72.650 kb on - strand, within A4249_RS00425 at 72.650 kb on - strand, within A4249_RS00425 at 72.797 kb on + strand, within A4249_RS00430 at 72.827 kb on + strand, within A4249_RS00430 at 72.828 kb on - strand, within A4249_RS00430 at 72.828 kb on - strand, within A4249_RS00430 at 72.845 kb on + strand, within A4249_RS00430 at 72.846 kb on - strand, within A4249_RS00430 at 72.846 kb on - strand, within A4249_RS00430 at 72.853 kb on + strand, within A4249_RS00430 at 72.853 kb on + strand, within A4249_RS00430 at 72.853 kb on + strand, within A4249_RS00430 at 72.853 kb on + strand, within A4249_RS00430 at 72.854 kb on - strand, within A4249_RS00430 at 72.854 kb on - strand, within A4249_RS00430 at 72.854 kb on - strand, within A4249_RS00430 at 72.854 kb on - strand, within A4249_RS00430 at 73.095 kb on - strand, within A4249_RS00430 at 73.095 kb on - strand, within A4249_RS00430 at 73.095 kb on - strand, within A4249_RS00430 at 73.095 kb on - strand, within A4249_RS00430 at 73.095 kb on - strand, within A4249_RS00430
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Lysinibacillus_FW301-JW8 40.7 ppm (C) remove 70,229 + A4249_RS00410 0.84 +0.1 70,229 + A4249_RS00410 0.84 -0.8 70,229 + A4249_RS00410 0.84 -0.3 70,230 - A4249_RS00410 0.84 -0.2 70,230 - A4249_RS00410 0.84 -1.3 70,314 - -1.2 70,314 - +0.1 70,378 + -0.0 70,379 - +1.2 70,379 - -0.2 70,495 + A4249_RS00415 0.17 -0.2 70,748 + A4249_RS00415 0.52 -0.1 70,748 + A4249_RS00415 0.52 -1.0 70,749 - A4249_RS00415 0.52 -0.5 70,749 - A4249_RS00415 0.52 +0.1 70,749 - A4249_RS00415 0.52 -1.0 70,749 - A4249_RS00415 0.52 +0.2 70,922 + A4249_RS00415 0.77 +0.3 70,922 + A4249_RS00415 0.77 -1.6 70,922 + A4249_RS00415 0.77 +0.7 70,922 + A4249_RS00415 0.77 -0.0 70,923 - A4249_RS00415 0.77 +0.3 70,924 + A4249_RS00415 0.77 -2.0 70,924 + A4249_RS00415 0.77 -1.0 70,924 + A4249_RS00415 0.77 +0.6 70,924 + A4249_RS00415 0.77 -0.5 70,924 + A4249_RS00415 0.77 -2.2 70,924 + A4249_RS00415 0.77 +0.4 70,924 + A4249_RS00415 0.77 -1.8 70,924 + A4249_RS00415 0.77 +0.2 70,924 + A4249_RS00415 0.77 +0.3 70,924 + A4249_RS00415 0.77 +0.6 70,924 + A4249_RS00415 0.77 -0.3 70,925 - A4249_RS00415 0.77 -0.3 70,925 - A4249_RS00415 0.77 +0.4 70,925 - A4249_RS00415 0.77 -0.4 70,925 - A4249_RS00415 0.77 -0.4 70,925 - A4249_RS00415 0.77 +0.7 70,925 - A4249_RS00415 0.77 +0.6 70,925 - A4249_RS00415 0.77 +0.4 70,925 - A4249_RS00415 0.77 +1.0 70,925 - A4249_RS00415 0.77 -1.1 70,925 - A4249_RS00415 0.77 +0.6 70,958 - A4249_RS00415 0.82 +1.8 70,958 - A4249_RS00415 0.82 -0.0 71,047 - +0.1 71,098 + -0.9 71,098 + -0.1 71,098 + -1.0 71,098 + -0.4 71,098 + -0.2 71,098 + +0.5 71,098 + +0.5 71,098 + +0.5 71,098 + -1.1 71,098 + +0.5 71,099 - -0.9 71,099 - -0.7 71,099 - -1.1 71,099 - -1.3 71,099 - +0.5 71,099 - +0.1 71,117 + -0.4 71,117 + +2.3 71,117 + -0.1 71,117 + -1.1 71,117 + -0.8 71,118 - -0.5 71,118 - +0.6 71,118 - -0.3 71,118 - -0.5 71,118 - -0.2 71,118 - +0.1 71,118 - +1.0 71,283 + A4249_RS00420 0.12 -0.6 71,283 + A4249_RS00420 0.12 +0.8 71,284 - A4249_RS00420 0.12 -1.3 71,284 - A4249_RS00420 0.12 +0.3 71,284 - A4249_RS00420 0.12 -1.3 71,284 - A4249_RS00420 0.12 -0.3 71,284 - A4249_RS00420 0.12 -0.6 71,285 + A4249_RS00420 0.12 +0.8 71,285 + A4249_RS00420 0.12 +0.9 71,285 + A4249_RS00420 0.12 -0.2 71,286 - A4249_RS00420 0.12 -1.0 71,286 - A4249_RS00420 0.12 -0.1 71,286 - A4249_RS00420 0.12 -0.9 71,330 - A4249_RS00420 0.16 -0.2 71,412 + A4249_RS00420 0.24 -0.4 71,413 - A4249_RS00420 0.24 -0.6 71,413 - A4249_RS00420 0.24 -0.5 71,435 + A4249_RS00420 0.26 +1.3 71,435 + A4249_RS00420 0.26 -0.7 71,436 - A4249_RS00420 0.26 +0.5 71,436 - A4249_RS00420 0.26 +0.5 71,436 - A4249_RS00420 0.26 -0.1 71,436 - A4249_RS00420 0.26 -0.2 71,436 - A4249_RS00420 0.26 -0.7 71,436 - A4249_RS00420 0.26 +1.1 71,436 - A4249_RS00420 0.26 +0.9 71,436 - A4249_RS00420 0.26 +0.6 71,529 + A4249_RS00420 0.34 +1.4 71,529 + A4249_RS00420 0.34 -0.2 71,529 + A4249_RS00420 0.34 -0.5 71,529 + A4249_RS00420 0.34 +0.1 71,529 + A4249_RS00420 0.34 -1.0 71,553 + A4249_RS00420 0.36 -0.9 71,553 + A4249_RS00420 0.36 -0.3 71,553 + A4249_RS00420 0.36 +0.6 71,553 + A4249_RS00420 0.36 -0.3 71,553 + A4249_RS00420 0.36 -0.0 71,553 + A4249_RS00420 0.36 -1.7 71,553 + A4249_RS00420 0.36 -0.1 71,553 + A4249_RS00420 0.36 +0.1 71,553 + A4249_RS00420 0.36 +0.3 71,553 + A4249_RS00420 0.36 +0.6 71,553 + A4249_RS00420 0.36 +0.1 71,553 + A4249_RS00420 0.36 -0.6 71,553 + A4249_RS00420 0.36 +0.2 71,553 + A4249_RS00420 0.36 -0.7 71,553 + A4249_RS00420 0.36 -0.7 71,553 + A4249_RS00420 0.36 +0.3 71,553 + A4249_RS00420 0.36 -0.5 71,554 - A4249_RS00420 0.36 +0.5 71,554 - A4249_RS00420 0.36 +0.1 71,554 - A4249_RS00420 0.36 -0.5 71,554 - A4249_RS00420 0.36 +0.4 71,554 - A4249_RS00420 0.36 -0.2 71,554 - A4249_RS00420 0.36 +0.1 71,554 - A4249_RS00420 0.36 -0.2 71,554 - A4249_RS00420 0.36 -0.5 71,554 - A4249_RS00420 0.36 +1.1 71,554 - A4249_RS00420 0.36 +1.8 71,554 - A4249_RS00420 0.36 +0.8 71,554 - A4249_RS00420 0.36 -0.8 71,555 + A4249_RS00420 0.36 +0.3 71,555 + A4249_RS00420 0.36 +1.1 71,555 + A4249_RS00420 0.36 +0.7 71,555 + A4249_RS00420 0.36 -0.3 71,555 + A4249_RS00420 0.36 +0.1 71,555 + A4249_RS00420 0.36 -0.2 71,555 + A4249_RS00420 0.36 -0.4 71,556 - A4249_RS00420 0.37 +0.6 71,556 - A4249_RS00420 0.37 -0.9 71,556 - A4249_RS00420 0.37 -0.5 71,646 + A4249_RS00420 0.45 +1.1 71,646 + A4249_RS00420 0.45 -0.1 71,646 + A4249_RS00420 0.45 -0.6 71,646 + A4249_RS00420 0.45 +0.0 71,646 + A4249_RS00420 0.45 +0.3 71,647 - A4249_RS00420 0.45 +1.2 71,647 - A4249_RS00420 0.45 +1.5 71,647 - A4249_RS00420 0.45 -1.1 71,922 + A4249_RS00420 0.70 +0.7 71,922 + A4249_RS00420 0.70 +0.0 71,922 + A4249_RS00420 0.70 +0.4 71,922 + A4249_RS00420 0.70 +0.6 71,922 + A4249_RS00420 0.70 -0.2 71,923 - A4249_RS00420 0.70 -1.1 71,923 - A4249_RS00420 0.70 -0.3 71,923 - A4249_RS00420 0.70 +0.2 71,923 - A4249_RS00420 0.70 -1.0 72,135 + A4249_RS00420 0.89 -0.0 72,135 + A4249_RS00420 0.89 -0.0 72,135 + A4249_RS00420 0.89 +0.8 72,135 + A4249_RS00420 0.89 -0.6 72,135 + A4249_RS00420 0.89 +1.1 72,136 - A4249_RS00420 0.89 -0.3 72,136 - A4249_RS00420 0.89 +0.8 72,136 - A4249_RS00420 0.89 -0.4 72,136 - A4249_RS00420 0.89 -0.4 72,374 - A4249_RS00425 0.14 +1.3 72,374 - A4249_RS00425 0.14 -0.6 72,374 - A4249_RS00425 0.14 -0.0 72,649 + A4249_RS00425 0.86 -0.1 72,649 + A4249_RS00425 0.86 -1.0 72,650 - A4249_RS00425 0.86 +0.4 72,650 - A4249_RS00425 0.86 -0.5 72,650 - A4249_RS00425 0.86 -0.1 72,797 + A4249_RS00430 0.13 +0.0 72,827 + A4249_RS00430 0.17 -0.2 72,828 - A4249_RS00430 0.17 -0.2 72,828 - A4249_RS00430 0.17 +0.4 72,845 + A4249_RS00430 0.20 -1.2 72,846 - A4249_RS00430 0.20 -1.0 72,846 - A4249_RS00430 0.20 +0.5 72,853 + A4249_RS00430 0.21 -1.5 72,853 + A4249_RS00430 0.21 +0.4 72,853 + A4249_RS00430 0.21 -0.8 72,853 + A4249_RS00430 0.21 +0.2 72,854 - A4249_RS00430 0.21 +0.3 72,854 - A4249_RS00430 0.21 -0.7 72,854 - A4249_RS00430 0.21 +0.3 72,854 - A4249_RS00430 0.21 +0.6 73,095 - A4249_RS00430 0.56 -0.8 73,095 - A4249_RS00430 0.56 +1.0 73,095 - A4249_RS00430 0.56 +0.5 73,095 - A4249_RS00430 0.56 -1.1 73,095 - A4249_RS00430 0.56 +2.4
Or see this region's nucleotide sequence