Strain Fitness in Variovorax sp. SCN45 around GFF5827

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5825 and GFF5826 are separated by 10 nucleotidesGFF5826 and GFF5827 overlap by 4 nucleotidesGFF5827 and GFF5828 overlap by 4 nucleotidesGFF5828 and GFF5829 are separated by 12 nucleotides GFF5825 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 1,559 to 2,266 GFF5825 GFF5826 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,277 to 2,984 GFF5826 GFF5827 - FIG00452956: hypothetical protein, at 2,981 to 4,054 GFF5827 GFF5828 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 4,051 to 4,920 GFF5828 GFF5829 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 4,933 to 6,201 GFF5829 Position (kb) 2 3 4 5Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1.994 kb on + strand, within GFF5825at 1.994 kb on + strand, within GFF5825at 2.294 kb on - strandat 2.294 kb on - strandat 3.120 kb on + strand, within GFF5827at 3.121 kb on - strand, within GFF5827at 3.121 kb on - strand, within GFF5827at 3.201 kb on + strand, within GFF5827at 3.201 kb on + strand, within GFF5827at 3.201 kb on + strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.202 kb on - strand, within GFF5827at 3.489 kb on + strand, within GFF5827at 3.490 kb on - strand, within GFF5827at 3.490 kb on - strand, within GFF5827at 3.490 kb on - strand, within GFF5827at 3.645 kb on + strand, within GFF5827at 3.645 kb on + strand, within GFF5827at 3.645 kb on + strand, within GFF5827at 3.646 kb on - strand, within GFF5827at 3.646 kb on - strand, within GFF5827at 3.795 kb on + strand, within GFF5827at 3.795 kb on + strand, within GFF5827at 4.306 kb on + strand, within GFF5828at 4.306 kb on + strand, within GFF5828at 4.307 kb on - strand, within GFF5828at 4.586 kb on + strand, within GFF5828at 4.778 kb on + strand, within GFF5828at 4.778 kb on + strand, within GFF5828at 4.778 kb on + strand, within GFF5828at 4.778 kb on + strand, within GFF5828at 4.835 kb on + strandat 4.835 kb on + strandat 4.869 kb on - strandat 4.910 kb on + strandat 4.934 kb on + strandat 4.935 kb on - strandat 4.935 kb on - strandat 4.935 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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1,994 + GFF5825 0.61 +0.3
1,994 + GFF5825 0.61 +0.3
2,294 - -0.3
2,294 - +0.2
3,120 + GFF5827 0.13 -0.1
3,121 - GFF5827 0.13 +1.2
3,121 - GFF5827 0.13 -0.9
3,201 + GFF5827 0.20 +0.0
3,201 + GFF5827 0.20 +0.9
3,201 + GFF5827 0.20 +1.0
3,202 - GFF5827 0.21 -0.4
3,202 - GFF5827 0.21 +0.4
3,202 - GFF5827 0.21 -0.5
3,202 - GFF5827 0.21 -0.1
3,202 - GFF5827 0.21 -0.1
3,202 - GFF5827 0.21 +0.6
3,489 + GFF5827 0.47 -0.8
3,490 - GFF5827 0.47 -0.4
3,490 - GFF5827 0.47 -0.1
3,490 - GFF5827 0.47 +0.2
3,645 + GFF5827 0.62 +1.5
3,645 + GFF5827 0.62 -0.4
3,645 + GFF5827 0.62 -2.3
3,646 - GFF5827 0.62 +3.1
3,646 - GFF5827 0.62 -0.0
3,795 + GFF5827 0.76 +1.2
3,795 + GFF5827 0.76 -0.4
4,306 + GFF5828 0.29 +0.0
4,306 + GFF5828 0.29 +0.5
4,307 - GFF5828 0.29 -0.1
4,586 + GFF5828 0.61 -0.8
4,778 + GFF5828 0.84 +0.6
4,778 + GFF5828 0.84 -0.2
4,778 + GFF5828 0.84 +0.1
4,778 + GFF5828 0.84 +1.6
4,835 + -1.7
4,835 + -0.2
4,869 - +0.1
4,910 + +0.5
4,934 + +0.7
4,935 - -0.1
4,935 - -1.2
4,935 - +0.9

Or see this region's nucleotide sequence