Strain Fitness in Variovorax sp. SCN45 around GFF521

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF520 and GFF521 overlap by 4 nucleotidesGFF521 and GFF522 are separated by 20 nucleotides GFF520 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 559,342 to 560,868 GFF520 GFF521 - Secretion protein HlyD precursor, at 560,865 to 562,001 GFF521 GFF522 - RND efflux system, inner membrane transporter, at 562,022 to 565,138 GFF522 Position (kb) 560 561 562 563Strain fitness (log2 ratio) -1 0 1 2at 559.918 kb on + strand, within GFF520at 559.918 kb on + strand, within GFF520at 560.260 kb on + strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.366 kb on - strand, within GFF520at 560.485 kb on + strand, within GFF520at 560.486 kb on - strand, within GFF520at 560.486 kb on - strand, within GFF520at 560.506 kb on + strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.611 kb on + strand, within GFF520at 560.611 kb on + strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.644 kb on + strand, within GFF520at 560.645 kb on - strand, within GFF520at 560.761 kb on + strandat 561.163 kb on + strand, within GFF521at 561.163 kb on + strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.324 kb on + strand, within GFF521at 561.325 kb on - strand, within GFF521at 561.325 kb on - strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.999 kb on + strandat 561.999 kb on + strandat 562.000 kb on - strandat 562.000 kb on - strandat 562.044 kb on + strandat 562.044 kb on + strandat 562.487 kb on + strand, within GFF522at 562.487 kb on + strand, within GFF522at 562.487 kb on + strand, within GFF522at 562.487 kb on + strand, within GFF522at 562.487 kb on + strand, within GFF522at 562.488 kb on - strand, within GFF522at 562.488 kb on - strand, within GFF522at 562.488 kb on - strand, within GFF522at 562.488 kb on - strand, within GFF522at 562.803 kb on + strand, within GFF522

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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559,918 + GFF520 0.38 +0.2
559,918 + GFF520 0.38 +0.1
560,260 + GFF520 0.60 -1.5
560,261 - GFF520 0.60 -0.7
560,261 - GFF520 0.60 -0.8
560,261 - GFF520 0.60 +0.4
560,261 - GFF520 0.60 -0.6
560,261 - GFF520 0.60 +0.8
560,366 - GFF520 0.67 -0.2
560,485 + GFF520 0.75 +0.1
560,486 - GFF520 0.75 -0.2
560,486 - GFF520 0.75 +0.4
560,506 + GFF520 0.76 -0.6
560,507 - GFF520 0.76 -1.3
560,507 - GFF520 0.76 -0.4
560,507 - GFF520 0.76 +0.2
560,611 + GFF520 0.83 +0.1
560,611 + GFF520 0.83 +0.1
560,612 - GFF520 0.83 +0.0
560,612 - GFF520 0.83 +0.6
560,612 - GFF520 0.83 +0.7
560,612 - GFF520 0.83 -0.2
560,644 + GFF520 0.85 +0.1
560,645 - GFF520 0.85 +0.8
560,761 + +0.4
561,163 + GFF521 0.26 -0.3
561,163 + GFF521 0.26 -0.4
561,164 - GFF521 0.26 -0.8
561,164 - GFF521 0.26 -0.2
561,164 - GFF521 0.26 -0.1
561,324 + GFF521 0.40 +0.5
561,325 - GFF521 0.40 +0.8
561,325 - GFF521 0.40 -1.5
561,669 + GFF521 0.71 -0.1
561,669 + GFF521 0.71 -0.5
561,669 + GFF521 0.71 -0.6
561,669 + GFF521 0.71 -1.1
561,669 + GFF521 0.71 +1.4
561,669 + GFF521 0.71 -0.1
561,670 - GFF521 0.71 +0.4
561,670 - GFF521 0.71 -0.8
561,670 - GFF521 0.71 +1.8
561,670 - GFF521 0.71 +0.3
561,670 - GFF521 0.71 -0.2
561,670 - GFF521 0.71 -0.6
561,670 - GFF521 0.71 -1.6
561,999 + +0.0
561,999 + +0.2
562,000 - -0.5
562,000 - -1.2
562,044 + +0.3
562,044 + -0.0
562,487 + GFF522 0.15 +0.4
562,487 + GFF522 0.15 -1.6
562,487 + GFF522 0.15 -0.0
562,487 + GFF522 0.15 -0.0
562,487 + GFF522 0.15 +0.5
562,488 - GFF522 0.15 +0.8
562,488 - GFF522 0.15 -0.2
562,488 - GFF522 0.15 -0.6
562,488 - GFF522 0.15 +0.0
562,803 + GFF522 0.25 -0.6

Or see this region's nucleotide sequence