Strain Fitness in Variovorax sp. SCN45 around GFF4578

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4576 and GFF4577 are separated by 38 nucleotidesGFF4577 and GFF4578 are separated by 147 nucleotidesGFF4578 and GFF4579 are separated by 14 nucleotides GFF4576 - BUG/TctC family periplasmic protein, at 215,421 to 216,422 GFF4576 GFF4577 - Electron transfer flavoprotein, beta subunit, at 216,461 to 217,210 GFF4577 GFF4578 - Electron transfer flavoprotein, alpha subunit, at 217,358 to 218,290 GFF4578 GFF4579 - putative oxidoreductase, nitronate monooxygenase family, at 218,305 to 219,276 GFF4579 Position (kb) 217 218 219Strain fitness (log2 ratio) -2 -1 0 1at 216.361 kb on - strandat 216.361 kb on - strandat 216.361 kb on - strandat 216.361 kb on - strandat 216.420 kb on + strandat 216.421 kb on - strandat 216.421 kb on - strandat 216.421 kb on - strandat 216.421 kb on - strandat 216.498 kb on - strandat 217.243 kb on + strandat 217.373 kb on - strandat 217.608 kb on - strand, within GFF4578at 218.119 kb on + strand, within GFF4578at 218.120 kb on + strand, within GFF4578at 218.120 kb on + strand, within GFF4578at 218.120 kb on + strand, within GFF4578at 218.120 kb on + strand, within GFF4578at 218.288 kb on + strandat 218.288 kb on + strandat 218.288 kb on + strandat 218.558 kb on - strand, within GFF4579at 218.558 kb on - strand, within GFF4579at 219.198 kb on + strandat 219.198 kb on + strandat 219.199 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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216,361 - -0.5
216,361 - +0.4
216,361 - +0.2
216,361 - +0.2
216,420 + -0.5
216,421 - +0.1
216,421 - -0.9
216,421 - +0.2
216,421 - +0.2
216,498 - -1.8
217,243 + +0.5
217,373 - -0.3
217,608 - GFF4578 0.27 -0.4
218,119 + GFF4578 0.82 -0.0
218,120 + GFF4578 0.82 -0.6
218,120 + GFF4578 0.82 -1.0
218,120 + GFF4578 0.82 -0.5
218,120 + GFF4578 0.82 -0.4
218,288 + -0.2
218,288 + -0.7
218,288 + -1.6
218,558 - GFF4579 0.26 -1.6
218,558 - GFF4579 0.26 +0.0
219,198 + +0.3
219,198 + -0.5
219,199 - -1.1

Or see this region's nucleotide sequence