Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4576 and GFF4577 are separated by 38 nucleotides GFF4577 and GFF4578 are separated by 147 nucleotides GFF4578 and GFF4579 are separated by 14 nucleotides
GFF4576 - BUG/TctC family periplasmic protein, at 215,421 to 216,422
GFF4576
GFF4577 - Electron transfer flavoprotein, beta subunit, at 216,461 to 217,210
GFF4577
GFF4578 - Electron transfer flavoprotein, alpha subunit, at 217,358 to 218,290
GFF4578
GFF4579 - putative oxidoreductase, nitronate monooxygenase family, at 218,305 to 219,276
GFF4579
Position (kb)
217
218
219 Strain fitness (log2 ratio)
-2
-1
0
1 at 216.361 kb on - strand at 216.361 kb on - strand at 216.361 kb on - strand at 216.361 kb on - strand at 216.420 kb on + strand at 216.421 kb on - strand at 216.421 kb on - strand at 216.421 kb on - strand at 216.421 kb on - strand at 216.498 kb on - strand at 217.243 kb on + strand at 217.373 kb on - strand at 217.608 kb on - strand, within GFF4578 at 218.119 kb on + strand, within GFF4578 at 218.120 kb on + strand, within GFF4578 at 218.120 kb on + strand, within GFF4578 at 218.120 kb on + strand, within GFF4578 at 218.120 kb on + strand, within GFF4578 at 218.288 kb on + strand at 218.288 kb on + strand at 218.288 kb on + strand at 218.558 kb on - strand, within GFF4579 at 218.558 kb on - strand, within GFF4579 at 219.198 kb on + strand at 219.198 kb on + strand at 219.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 216,361 - -0.5 216,361 - +0.4 216,361 - +0.2 216,361 - +0.2 216,420 + -0.5 216,421 - +0.1 216,421 - -0.9 216,421 - +0.2 216,421 - +0.2 216,498 - -1.8 217,243 + +0.5 217,373 - -0.3 217,608 - GFF4578 0.27 -0.4 218,119 + GFF4578 0.82 -0.0 218,120 + GFF4578 0.82 -0.6 218,120 + GFF4578 0.82 -1.0 218,120 + GFF4578 0.82 -0.5 218,120 + GFF4578 0.82 -0.4 218,288 + -0.2 218,288 + -0.7 218,288 + -1.6 218,558 - GFF4579 0.26 -1.6 218,558 - GFF4579 0.26 +0.0 219,198 + +0.3 219,198 + -0.5 219,199 - -1.1
Or see this region's nucleotide sequence