Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2006 and GFF2007 are separated by 143 nucleotides GFF2007 and GFF2008 overlap by 4 nucleotides
GFF2006 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 49,632 to 50,804
GFF2006
GFF2007 - Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16), at 50,948 to 52,627
GFF2007
GFF2008 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 52,624 to 54,252
GFF2008
Position (kb)
50
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 50.085 kb on + strand, within GFF2006 at 50.085 kb on + strand, within GFF2006 at 50.086 kb on - strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.544 kb on + strand, within GFF2006 at 50.682 kb on + strand, within GFF2006 at 50.730 kb on + strand at 50.731 kb on - strand at 50.731 kb on - strand at 50.731 kb on - strand at 50.738 kb on - strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.979 kb on - strand at 50.979 kb on - strand at 50.979 kb on - strand at 51.105 kb on - strand at 51.105 kb on - strand at 51.272 kb on + strand, within GFF2007 at 51.273 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.356 kb on + strand, within GFF2007 at 51.357 kb on - strand, within GFF2007 at 51.357 kb on - strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.690 kb on - strand, within GFF2007 at 51.753 kb on - strand, within GFF2007 at 51.983 kb on + strand, within GFF2007 at 52.157 kb on + strand, within GFF2007 at 52.158 kb on - strand, within GFF2007 at 52.265 kb on + strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.322 kb on + strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.349 kb on + strand, within GFF2007 at 52.350 kb on - strand, within GFF2007 at 52.404 kb on - strand, within GFF2007 at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.514 kb on + strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.991 kb on - strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.219 kb on - strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 49,974 + GFF2006 0.29 +0.5 49,974 + GFF2006 0.29 -0.5 49,974 + GFF2006 0.29 -0.8 49,974 + GFF2006 0.29 +0.0 49,974 + GFF2006 0.29 -0.2 49,974 + GFF2006 0.29 -0.8 49,975 - GFF2006 0.29 -0.9 49,975 - GFF2006 0.29 +1.3 49,975 - GFF2006 0.29 -0.6 49,975 - GFF2006 0.29 -0.3 49,975 - GFF2006 0.29 -0.4 49,975 - GFF2006 0.29 -0.5 50,085 + GFF2006 0.39 +0.4 50,085 + GFF2006 0.39 -0.8 50,086 - GFF2006 0.39 +0.1 50,088 + GFF2006 0.39 -1.0 50,088 + GFF2006 0.39 +0.4 50,088 + GFF2006 0.39 -2.3 50,088 + GFF2006 0.39 -1.2 50,088 + GFF2006 0.39 -0.2 50,088 + GFF2006 0.39 -0.0 50,088 + GFF2006 0.39 -0.2 50,088 + GFF2006 0.39 -0.7 50,088 + GFF2006 0.39 -0.8 50,088 + GFF2006 0.39 +0.5 50,089 - GFF2006 0.39 -1.3 50,089 - GFF2006 0.39 -0.2 50,089 - GFF2006 0.39 +0.6 50,089 - GFF2006 0.39 +0.4 50,089 - GFF2006 0.39 -0.2 50,089 - GFF2006 0.39 -0.7 50,089 - GFF2006 0.39 +0.3 50,089 - GFF2006 0.39 -0.1 50,089 - GFF2006 0.39 -1.0 50,089 - GFF2006 0.39 -0.1 50,089 - GFF2006 0.39 +0.0 50,089 - GFF2006 0.39 -0.0 50,151 + GFF2006 0.44 -1.2 50,151 + GFF2006 0.44 -1.8 50,151 + GFF2006 0.44 -0.5 50,152 - GFF2006 0.44 -0.0 50,152 - GFF2006 0.44 +0.5 50,152 - GFF2006 0.44 -0.7 50,511 + GFF2006 0.75 -0.6 50,511 + GFF2006 0.75 +0.2 50,511 + GFF2006 0.75 -0.2 50,511 + GFF2006 0.75 -0.0 50,511 + GFF2006 0.75 -1.8 50,511 + GFF2006 0.75 +1.0 50,511 + GFF2006 0.75 +0.2 50,511 + GFF2006 0.75 +0.2 50,511 + GFF2006 0.75 -1.0 50,511 + GFF2006 0.75 +0.6 50,511 + GFF2006 0.75 +0.2 50,511 + GFF2006 0.75 -1.0 50,511 + GFF2006 0.75 -0.4 50,511 + GFF2006 0.75 -1.9 50,511 + GFF2006 0.75 -0.9 50,511 + GFF2006 0.75 -0.8 50,511 + GFF2006 0.75 -0.4 50,511 + GFF2006 0.75 +0.4 50,512 - GFF2006 0.75 -0.4 50,512 - GFF2006 0.75 -0.6 50,512 - GFF2006 0.75 -0.3 50,512 - GFF2006 0.75 -0.6 50,512 - GFF2006 0.75 +0.6 50,512 - GFF2006 0.75 -0.3 50,512 - GFF2006 0.75 -1.0 50,538 + GFF2006 0.77 -0.1 50,538 + GFF2006 0.77 -0.8 50,538 + GFF2006 0.77 -0.7 50,544 + GFF2006 0.78 -0.6 50,682 + GFF2006 0.90 -0.8 50,730 + -2.6 50,731 - -1.0 50,731 - +0.0 50,731 - -0.0 50,738 - -0.6 50,802 + -0.5 50,802 + -0.6 50,802 + -1.6 50,802 + -0.3 50,802 + -0.9 50,802 + -1.2 50,802 + -0.6 50,802 + -0.1 50,803 - -0.5 50,803 - +1.6 50,803 - -0.4 50,803 - +1.8 50,979 - +0.3 50,979 - -1.9 50,979 - -0.8 51,105 - -0.5 51,105 - -0.2 51,272 + GFF2007 0.19 -0.7 51,273 - GFF2007 0.19 -0.6 51,294 - GFF2007 0.21 -0.1 51,294 - GFF2007 0.21 -0.6 51,294 - GFF2007 0.21 -0.5 51,356 + GFF2007 0.24 -0.7 51,357 - GFF2007 0.24 -0.6 51,357 - GFF2007 0.24 -0.4 51,689 + GFF2007 0.44 -0.0 51,689 + GFF2007 0.44 +0.8 51,689 + GFF2007 0.44 -0.9 51,690 - GFF2007 0.44 -0.9 51,753 - GFF2007 0.48 +0.2 51,983 + GFF2007 0.62 -0.2 52,157 + GFF2007 0.72 -0.2 52,158 - GFF2007 0.72 -1.4 52,265 + GFF2007 0.78 -0.1 52,266 - GFF2007 0.78 -0.1 52,266 - GFF2007 0.78 +0.2 52,266 - GFF2007 0.78 -0.5 52,266 - GFF2007 0.78 +0.1 52,266 - GFF2007 0.78 -0.6 52,266 - GFF2007 0.78 +0.2 52,322 + GFF2007 0.82 +0.8 52,323 - GFF2007 0.82 -0.8 52,323 - GFF2007 0.82 +0.2 52,323 - GFF2007 0.82 -0.0 52,323 - GFF2007 0.82 +0.4 52,323 - GFF2007 0.82 +0.3 52,349 + GFF2007 0.83 +1.0 52,350 - GFF2007 0.83 -0.1 52,404 - GFF2007 0.87 -0.4 52,499 + +0.2 52,499 + -0.5 52,499 + -0.3 52,499 + -0.5 52,499 + -0.7 52,499 + -0.5 52,499 + -0.4 52,499 + +0.0 52,499 + +0.2 52,500 - -0.7 52,500 - -0.1 52,500 - -0.2 52,500 - -1.2 52,500 - -0.8 52,500 - -0.3 52,500 - -0.3 52,500 - -0.0 52,500 - -0.6 52,514 + +0.1 52,515 - -0.8 52,515 - -0.2 52,515 - -0.4 52,515 - -0.8 52,867 + GFF2008 0.15 -0.9 52,867 + GFF2008 0.15 -0.3 52,867 + GFF2008 0.15 -0.9 52,867 + GFF2008 0.15 -0.8 52,868 - GFF2008 0.15 +1.5 52,868 - GFF2008 0.15 +0.2 52,987 + GFF2008 0.22 +1.0 52,987 + GFF2008 0.22 -0.4 52,987 + GFF2008 0.22 -0.2 52,987 + GFF2008 0.22 -0.2 52,987 + GFF2008 0.22 -0.5 52,987 + GFF2008 0.22 +0.8 52,987 + GFF2008 0.22 +0.4 52,987 + GFF2008 0.22 +0.5 52,987 + GFF2008 0.22 +0.8 52,988 - GFF2008 0.22 +0.1 52,988 - GFF2008 0.22 +0.0 52,988 - GFF2008 0.22 -1.4 52,988 - GFF2008 0.22 -1.6 52,988 - GFF2008 0.22 -0.5 52,988 - GFF2008 0.22 -0.2 52,988 - GFF2008 0.22 +0.1 52,991 - GFF2008 0.23 -0.0 53,059 + GFF2008 0.27 -1.2 53,059 + GFF2008 0.27 +0.1 53,059 + GFF2008 0.27 +0.8 53,059 + GFF2008 0.27 -0.5 53,060 - GFF2008 0.27 -0.4 53,060 - GFF2008 0.27 +0.1 53,060 - GFF2008 0.27 -1.7 53,140 + GFF2008 0.32 +0.5 53,140 + GFF2008 0.32 +0.3 53,140 + GFF2008 0.32 +0.4 53,141 - GFF2008 0.32 -1.0 53,141 - GFF2008 0.32 -0.7 53,141 - GFF2008 0.32 -0.2 53,141 - GFF2008 0.32 -0.6 53,141 - GFF2008 0.32 -0.9 53,218 + GFF2008 0.36 -1.6 53,218 + GFF2008 0.36 +1.8 53,219 - GFF2008 0.37 -0.8 53,512 + GFF2008 0.55 -0.6 53,512 + GFF2008 0.55 +1.0 53,512 + GFF2008 0.55 -0.8 53,512 + GFF2008 0.55 +0.8 53,512 + GFF2008 0.55 -1.2 53,512 + GFF2008 0.55 +0.2
Or see this region's nucleotide sequence