Strain Fitness in Variovorax sp. SCN45 around GFF61

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF60 and GFF61 overlap by 8 nucleotidesGFF61 and GFF62 are separated by 16 nucleotides GFF60 - GDP-L-fucose synthetase (EC 1.1.1.271), at 75,995 to 76,942 GFF60 GFF61 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 76,935 to 78,059 GFF61 GFF62 - hypothetical protein, at 78,076 to 79,422 GFF62 Position (kb) 76 77 78 79Strain fitness (log2 ratio) -2 -1 0 1at 75.965 kb on - strandat 76.130 kb on + strand, within GFF60at 76.130 kb on + strand, within GFF60at 76.130 kb on + strand, within GFF60at 76.131 kb on - strand, within GFF60at 76.131 kb on - strand, within GFF60at 76.175 kb on + strand, within GFF60at 76.176 kb on - strand, within GFF60at 76.193 kb on + strand, within GFF60at 76.194 kb on - strand, within GFF60at 76.283 kb on + strand, within GFF60at 76.283 kb on + strand, within GFF60at 76.283 kb on + strand, within GFF60at 76.283 kb on + strand, within GFF60at 76.284 kb on - strand, within GFF60at 76.358 kb on + strand, within GFF60at 76.359 kb on - strand, within GFF60at 76.400 kb on + strand, within GFF60at 76.400 kb on + strand, within GFF60at 76.400 kb on + strand, within GFF60at 76.436 kb on + strand, within GFF60at 76.437 kb on - strand, within GFF60at 76.464 kb on - strand, within GFF60at 76.481 kb on + strand, within GFF60at 76.481 kb on + strand, within GFF60at 76.482 kb on - strand, within GFF60at 76.482 kb on - strand, within GFF60at 76.482 kb on - strand, within GFF60at 76.601 kb on + strand, within GFF60at 76.601 kb on + strand, within GFF60at 76.659 kb on - strand, within GFF60at 76.983 kb on + strandat 76.984 kb on - strandat 76.984 kb on - strandat 76.984 kb on - strandat 77.077 kb on - strand, within GFF61at 77.187 kb on + strand, within GFF61at 77.188 kb on - strand, within GFF61at 77.322 kb on + strand, within GFF61at 77.323 kb on - strand, within GFF61at 77.323 kb on - strand, within GFF61at 77.347 kb on - strand, within GFF61at 77.392 kb on - strand, within GFF61at 77.424 kb on + strand, within GFF61at 77.424 kb on + strand, within GFF61at 77.448 kb on + strand, within GFF61at 77.449 kb on - strand, within GFF61at 77.460 kb on + strand, within GFF61at 77.461 kb on - strand, within GFF61at 77.461 kb on - strand, within GFF61at 77.461 kb on - strand, within GFF61at 77.469 kb on + strand, within GFF61at 77.470 kb on - strand, within GFF61at 77.634 kb on + strand, within GFF61at 77.634 kb on + strand, within GFF61at 77.682 kb on + strand, within GFF61at 77.683 kb on - strand, within GFF61at 77.683 kb on - strand, within GFF61at 77.706 kb on + strand, within GFF61at 78.247 kb on + strand, within GFF62at 78.356 kb on - strand, within GFF62at 78.356 kb on - strand, within GFF62at 78.356 kb on - strand, within GFF62at 78.356 kb on - strand, within GFF62at 78.356 kb on - strand, within GFF62at 78.356 kb on - strand, within GFF62

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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75,965 - -0.0
76,130 + GFF60 0.14 -0.7
76,130 + GFF60 0.14 +1.0
76,130 + GFF60 0.14 +0.8
76,131 - GFF60 0.14 +1.3
76,131 - GFF60 0.14 -0.7
76,175 + GFF60 0.19 -1.1
76,176 - GFF60 0.19 +0.1
76,193 + GFF60 0.21 -0.3
76,194 - GFF60 0.21 +0.0
76,283 + GFF60 0.30 -0.5
76,283 + GFF60 0.30 -0.3
76,283 + GFF60 0.30 -0.8
76,283 + GFF60 0.30 +0.0
76,284 - GFF60 0.30 -0.3
76,358 + GFF60 0.38 +1.6
76,359 - GFF60 0.38 -0.3
76,400 + GFF60 0.43 -0.2
76,400 + GFF60 0.43 +0.4
76,400 + GFF60 0.43 -2.2
76,436 + GFF60 0.47 -0.2
76,437 - GFF60 0.47 +0.2
76,464 - GFF60 0.49 +0.5
76,481 + GFF60 0.51 +0.4
76,481 + GFF60 0.51 +0.1
76,482 - GFF60 0.51 -0.6
76,482 - GFF60 0.51 -0.8
76,482 - GFF60 0.51 +0.7
76,601 + GFF60 0.64 -0.0
76,601 + GFF60 0.64 +0.6
76,659 - GFF60 0.70 +0.3
76,983 + +0.4
76,984 - -0.3
76,984 - +0.3
76,984 - -0.2
77,077 - GFF61 0.13 -0.4
77,187 + GFF61 0.22 +0.3
77,188 - GFF61 0.22 -1.3
77,322 + GFF61 0.34 -0.5
77,323 - GFF61 0.34 -0.8
77,323 - GFF61 0.34 +1.7
77,347 - GFF61 0.37 +0.8
77,392 - GFF61 0.41 -0.2
77,424 + GFF61 0.43 -1.2
77,424 + GFF61 0.43 +0.6
77,448 + GFF61 0.46 -0.3
77,449 - GFF61 0.46 -1.5
77,460 + GFF61 0.47 -0.1
77,461 - GFF61 0.47 -0.3
77,461 - GFF61 0.47 +0.3
77,461 - GFF61 0.47 +0.7
77,469 + GFF61 0.47 +0.1
77,470 - GFF61 0.48 +0.2
77,634 + GFF61 0.62 +0.6
77,634 + GFF61 0.62 +0.3
77,682 + GFF61 0.66 -0.1
77,683 - GFF61 0.66 +0.6
77,683 - GFF61 0.66 -0.3
77,706 + GFF61 0.69 +0.3
78,247 + GFF62 0.13 +1.3
78,356 - GFF62 0.21 +0.8
78,356 - GFF62 0.21 +0.3
78,356 - GFF62 0.21 +0.3
78,356 - GFF62 0.21 -0.1
78,356 - GFF62 0.21 -0.5
78,356 - GFF62 0.21 +0.2

Or see this region's nucleotide sequence