Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4365 and GFF4366 overlap by 4 nucleotides GFF4366 and GFF4367 are separated by 7 nucleotides GFF4367 and GFF4368 are separated by 35 nucleotides
GFF4365 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 287,788 to 289,794
GFF4365
GFF4366 - BUG/TctC family periplasmic protein, at 289,791 to 290,777
GFF4366
GFF4367 - KEGG: regulator of ribonuclease activity A, at 290,785 to 291,270
GFF4367
GFF4368 - Peptidoglycan D,D-transpeptidase MrdA (EC 3.4.16.4), at 291,306 to 293,294
GFF4368
Position (kb) 289
290
291 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 288.793 kb on + strand, within GFF4365 at 288.793 kb on + strand, within GFF4365 at 290.115 kb on + strand, within GFF4366 at 290.115 kb on + strand, within GFF4366 at 290.116 kb on - strand, within GFF4366 at 290.238 kb on + strand, within GFF4366 at 290.268 kb on + strand, within GFF4366 at 290.269 kb on - strand, within GFF4366 at 290.498 kb on - strand, within GFF4366 at 290.541 kb on + strand, within GFF4366 at 291.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 288,793 + GFF4365 0.50 -0.4 288,793 + GFF4365 0.50 +0.8 290,115 + GFF4366 0.33 +0.8 290,115 + GFF4366 0.33 +3.2 290,116 - GFF4366 0.33 +0.7 290,238 + GFF4366 0.45 +0.8 290,268 + GFF4366 0.48 -1.2 290,269 - GFF4366 0.48 -0.0 290,498 - GFF4366 0.72 -0.3 290,541 + GFF4366 0.76 -2.1 291,288 - +0.1
Or see this region's nucleotide sequence