Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2007 and GFF2008 overlap by 4 nucleotides GFF2008 and GFF2009 are separated by 21 nucleotides GFF2009 and GFF2010 are separated by 16 nucleotides
GFF2007 - Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16), at 50,948 to 52,627
GFF2007
GFF2008 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 52,624 to 54,252
GFF2008
GFF2009 - Dienelactone hydrolase and related enzymes, at 54,274 to 55,140
GFF2009
GFF2010 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 55,157 to 55,963
GFF2010
Position (kb)
52
53
54
55 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.690 kb on - strand, within GFF2007 at 51.753 kb on - strand, within GFF2007 at 51.983 kb on + strand, within GFF2007 at 52.157 kb on + strand, within GFF2007 at 52.158 kb on - strand, within GFF2007 at 52.265 kb on + strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.322 kb on + strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.349 kb on + strand, within GFF2007 at 52.350 kb on - strand, within GFF2007 at 52.404 kb on - strand, within GFF2007 at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.514 kb on + strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.991 kb on - strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.219 kb on - strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.797 kb on + strand, within GFF2008 at 53.797 kb on + strand, within GFF2008 at 53.908 kb on + strand, within GFF2008 at 53.908 kb on + strand, within GFF2008 at 53.908 kb on + strand, within GFF2008 at 53.908 kb on + strand, within GFF2008 at 53.908 kb on + strand, within GFF2008 at 53.909 kb on - strand, within GFF2008 at 53.929 kb on + strand, within GFF2008 at 53.929 kb on + strand, within GFF2008 at 53.930 kb on - strand, within GFF2008 at 54.097 kb on + strand at 54.098 kb on - strand at 54.098 kb on - strand at 54.118 kb on + strand at 54.118 kb on + strand at 54.118 kb on + strand at 54.118 kb on + strand at 54.119 kb on - strand at 54.119 kb on - strand at 54.121 kb on + strand at 54.121 kb on + strand at 54.121 kb on + strand at 54.121 kb on + strand at 54.122 kb on - strand at 54.122 kb on - strand at 54.122 kb on - strand at 54.122 kb on - strand at 54.122 kb on - strand at 54.122 kb on - strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.250 kb on + strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.251 kb on - strand at 54.427 kb on + strand, within GFF2009 at 54.427 kb on + strand, within GFF2009 at 54.427 kb on + strand, within GFF2009 at 54.427 kb on + strand, within GFF2009 at 54.427 kb on + strand, within GFF2009 at 54.428 kb on - strand, within GFF2009 at 54.521 kb on - strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.619 kb on + strand, within GFF2009 at 54.620 kb on - strand, within GFF2009 at 54.643 kb on + strand, within GFF2009 at 54.644 kb on - strand, within GFF2009 at 54.644 kb on - strand, within GFF2009 at 54.644 kb on - strand, within GFF2009 at 54.694 kb on + strand, within GFF2009 at 54.713 kb on - strand, within GFF2009 at 54.893 kb on - strand, within GFF2009 at 54.928 kb on + strand, within GFF2009 at 54.928 kb on + strand, within GFF2009 at 54.929 kb on - strand, within GFF2009 at 54.985 kb on + strand, within GFF2009 at 54.985 kb on + strand, within GFF2009 at 55.172 kb on + strand at 55.172 kb on + strand at 55.172 kb on + strand at 55.173 kb on - strand at 55.173 kb on - strand at 55.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 51,689 + GFF2007 0.44 +0.2 51,689 + GFF2007 0.44 +0.6 51,689 + GFF2007 0.44 -1.4 51,690 - GFF2007 0.44 +0.1 51,753 - GFF2007 0.48 +1.1 51,983 + GFF2007 0.62 +2.0 52,157 + GFF2007 0.72 -1.4 52,158 - GFF2007 0.72 +0.5 52,265 + GFF2007 0.78 -0.1 52,266 - GFF2007 0.78 -0.4 52,266 - GFF2007 0.78 +0.4 52,266 - GFF2007 0.78 -0.2 52,266 - GFF2007 0.78 -0.2 52,266 - GFF2007 0.78 -0.1 52,266 - GFF2007 0.78 +0.1 52,322 + GFF2007 0.82 -0.9 52,323 - GFF2007 0.82 -0.1 52,323 - GFF2007 0.82 -0.6 52,323 - GFF2007 0.82 -0.2 52,323 - GFF2007 0.82 +0.6 52,323 - GFF2007 0.82 +0.2 52,349 + GFF2007 0.83 -0.2 52,350 - GFF2007 0.83 -0.1 52,404 - GFF2007 0.87 -0.5 52,499 + -0.4 52,499 + +0.1 52,499 + +0.1 52,499 + -0.1 52,499 + -0.0 52,499 + -0.8 52,499 + -0.0 52,499 + -0.1 52,499 + -0.6 52,500 - +0.2 52,500 - +0.1 52,500 - -0.3 52,500 - -0.5 52,500 - -0.9 52,500 - +0.3 52,500 - -0.3 52,500 - -0.1 52,500 - -1.2 52,514 + -0.4 52,515 - +0.6 52,515 - -0.2 52,515 - -0.5 52,515 - -0.1 52,867 + GFF2008 0.15 -1.0 52,867 + GFF2008 0.15 -0.4 52,867 + GFF2008 0.15 -0.0 52,867 + GFF2008 0.15 -1.9 52,868 - GFF2008 0.15 +0.4 52,868 - GFF2008 0.15 -0.2 52,987 + GFF2008 0.22 -0.9 52,987 + GFF2008 0.22 -0.2 52,987 + GFF2008 0.22 -0.4 52,987 + GFF2008 0.22 -0.4 52,987 + GFF2008 0.22 -0.5 52,987 + GFF2008 0.22 +1.1 52,987 + GFF2008 0.22 +0.3 52,987 + GFF2008 0.22 -0.4 52,987 + GFF2008 0.22 +0.6 52,988 - GFF2008 0.22 -0.2 52,988 - GFF2008 0.22 -0.1 52,988 - GFF2008 0.22 +0.5 52,988 - GFF2008 0.22 -1.1 52,988 - GFF2008 0.22 -0.6 52,988 - GFF2008 0.22 +0.6 52,988 - GFF2008 0.22 -0.3 52,991 - GFF2008 0.23 -0.2 53,059 + GFF2008 0.27 +0.2 53,059 + GFF2008 0.27 -0.2 53,059 + GFF2008 0.27 +1.0 53,059 + GFF2008 0.27 -0.6 53,060 - GFF2008 0.27 -0.9 53,060 - GFF2008 0.27 -0.5 53,060 - GFF2008 0.27 -0.8 53,140 + GFF2008 0.32 -0.2 53,140 + GFF2008 0.32 -0.8 53,140 + GFF2008 0.32 -1.1 53,141 - GFF2008 0.32 +0.2 53,141 - GFF2008 0.32 -1.4 53,141 - GFF2008 0.32 +1.2 53,141 - GFF2008 0.32 +0.3 53,141 - GFF2008 0.32 -0.2 53,218 + GFF2008 0.36 +1.0 53,218 + GFF2008 0.36 +0.1 53,219 - GFF2008 0.37 -0.9 53,512 + GFF2008 0.55 -0.7 53,512 + GFF2008 0.55 +0.3 53,512 + GFF2008 0.55 -0.4 53,512 + GFF2008 0.55 +0.1 53,512 + GFF2008 0.55 -1.4 53,512 + GFF2008 0.55 +0.3 53,797 + GFF2008 0.72 -0.6 53,797 + GFF2008 0.72 +0.3 53,908 + GFF2008 0.79 -0.2 53,908 + GFF2008 0.79 -1.2 53,908 + GFF2008 0.79 -2.1 53,908 + GFF2008 0.79 -1.0 53,908 + GFF2008 0.79 +0.2 53,909 - GFF2008 0.79 -0.7 53,929 + GFF2008 0.80 -0.8 53,929 + GFF2008 0.80 -0.3 53,930 - GFF2008 0.80 -0.2 54,097 + +0.0 54,098 - +0.1 54,098 - +0.2 54,118 + -1.3 54,118 + -0.6 54,118 + -0.2 54,118 + -0.7 54,119 - -0.9 54,119 - -0.7 54,121 + -0.6 54,121 + +0.4 54,121 + +0.2 54,121 + -0.9 54,122 - +1.3 54,122 - -0.8 54,122 - -1.1 54,122 - -0.3 54,122 - -0.1 54,122 - -0.4 54,250 + -0.2 54,250 + +0.5 54,250 + -0.9 54,250 + -0.9 54,250 + -0.4 54,250 + -0.2 54,250 + +0.8 54,250 + -0.5 54,251 - -0.5 54,251 - -0.7 54,251 - -0.4 54,251 - +0.2 54,251 - +0.1 54,251 - -0.4 54,251 - +0.4 54,251 - +0.6 54,427 + GFF2009 0.18 -1.1 54,427 + GFF2009 0.18 -0.3 54,427 + GFF2009 0.18 +0.2 54,427 + GFF2009 0.18 -0.6 54,427 + GFF2009 0.18 -0.5 54,428 - GFF2009 0.18 -1.3 54,521 - GFF2009 0.28 -0.2 54,619 + GFF2009 0.40 -2.4 54,619 + GFF2009 0.40 -0.0 54,619 + GFF2009 0.40 +0.1 54,619 + GFF2009 0.40 -0.1 54,619 + GFF2009 0.40 -0.1 54,619 + GFF2009 0.40 -1.3 54,619 + GFF2009 0.40 -0.2 54,619 + GFF2009 0.40 -0.5 54,620 - GFF2009 0.40 -0.5 54,643 + GFF2009 0.43 -0.6 54,644 - GFF2009 0.43 +0.1 54,644 - GFF2009 0.43 -0.5 54,644 - GFF2009 0.43 -0.3 54,694 + GFF2009 0.48 +0.3 54,713 - GFF2009 0.51 -1.5 54,893 - GFF2009 0.71 -0.2 54,928 + GFF2009 0.75 -0.2 54,928 + GFF2009 0.75 +1.9 54,929 - GFF2009 0.76 +0.8 54,985 + GFF2009 0.82 +0.5 54,985 + GFF2009 0.82 -0.7 55,172 + -1.4 55,172 + +1.0 55,172 + +0.1 55,173 - -0.4 55,173 - -0.4 55,173 - -0.9
Or see this region's nucleotide sequence