Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF191 and GFF192 are separated by 117 nucleotides GFF192 and GFF193 overlap by 4 nucleotides GFF193 and GFF194 are separated by 35 nucleotides
GFF191 - (Y14336) putative extracellular protein containing predicted 35aa signal peptide, at 220,808 to 221,308
GFF191
GFF192 - Nitrogen regulation protein NR(I), GlnG (=NtrC), at 221,426 to 222,067
GFF192
GFF193 - Two-component sensor PilS, at 222,064 to 223,569
GFF193
GFF194 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 223,605 to 224,699
GFF194
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1 at 221.145 kb on + strand, within GFF191 at 221.145 kb on + strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.146 kb on - strand, within GFF191 at 221.370 kb on + strand at 221.371 kb on - strand at 221.399 kb on + strand at 221.400 kb on - strand at 221.421 kb on + strand at 221.422 kb on - strand at 221.427 kb on + strand at 221.428 kb on - strand at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.127 kb on + strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.128 kb on - strand, within GFF193 at 223.138 kb on + strand, within GFF193 at 223.139 kb on - strand, within GFF193 at 223.160 kb on + strand, within GFF193 at 223.403 kb on + strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.404 kb on - strand, within GFF193 at 223.642 kb on + strand at 223.642 kb on + strand at 223.642 kb on + strand at 223.643 kb on - strand at 223.643 kb on - strand at 223.774 kb on + strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.775 kb on - strand, within GFF194 at 223.830 kb on + strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.831 kb on - strand, within GFF194 at 223.867 kb on - strand, within GFF194 at 223.954 kb on - strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 223.977 kb on + strand, within GFF194 at 224.494 kb on - strand, within GFF194 at 224.514 kb on + strand, within GFF194 at 224.515 kb on - strand, within GFF194 at 224.515 kb on - strand, within GFF194
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 221,145 + GFF191 0.67 -1.8 221,145 + GFF191 0.67 -0.9 221,146 - GFF191 0.67 -0.5 221,146 - GFF191 0.67 +0.4 221,146 - GFF191 0.67 +0.0 221,146 - GFF191 0.67 -1.0 221,370 + +0.5 221,371 - +0.3 221,399 + -0.4 221,400 - +0.1 221,421 + -0.3 221,422 - +0.2 221,427 + -0.4 221,428 - -0.7 223,127 + GFF193 0.71 -0.7 223,127 + GFF193 0.71 +0.6 223,127 + GFF193 0.71 -0.8 223,127 + GFF193 0.71 +0.6 223,128 - GFF193 0.71 +0.2 223,128 - GFF193 0.71 -0.4 223,128 - GFF193 0.71 +0.7 223,128 - GFF193 0.71 -0.1 223,138 + GFF193 0.71 +0.4 223,139 - GFF193 0.71 +0.4 223,160 + GFF193 0.73 +1.1 223,403 + GFF193 0.89 +0.3 223,404 - GFF193 0.89 -0.1 223,404 - GFF193 0.89 +0.3 223,404 - GFF193 0.89 +0.5 223,642 + -0.0 223,642 + +0.1 223,642 + +0.1 223,643 - -0.3 223,643 - -0.3 223,774 + GFF194 0.15 -0.5 223,775 - GFF194 0.16 -0.5 223,775 - GFF194 0.16 -0.0 223,830 + GFF194 0.21 +0.2 223,831 - GFF194 0.21 +0.3 223,831 - GFF194 0.21 +0.8 223,831 - GFF194 0.21 +1.3 223,867 - GFF194 0.24 +0.9 223,954 - GFF194 0.32 -0.6 223,977 + GFF194 0.34 -0.5 223,977 + GFF194 0.34 -0.1 224,494 - GFF194 0.81 +0.7 224,514 + GFF194 0.83 +0.7 224,515 - GFF194 0.83 -0.6 224,515 - GFF194 0.83 +0.1
Or see this region's nucleotide sequence