Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF70 and GFF71 are separated by 6 nucleotides GFF71 and GFF72 are separated by 24 nucleotides
GFF70 - Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 86,892 to 88,403
GFF70
GFF71 - Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family), at 88,410 to 89,219
GFF71
GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377
GFF72
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 87.459 kb on + strand, within GFF70 at 87.991 kb on - strand, within GFF70 at 88.167 kb on + strand, within GFF70 at 88.168 kb on - strand, within GFF70 at 88.168 kb on - strand, within GFF70 at 88.287 kb on + strand at 88.287 kb on + strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.446 kb on + strand at 88.526 kb on - strand, within GFF71 at 88.526 kb on - strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.796 kb on - strand, within GFF71 at 89.232 kb on + strand at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 90.134 kb on - strand, within GFF72
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 87,459 + GFF70 0.38 -0.4 87,991 - GFF70 0.73 +0.5 88,167 + GFF70 0.84 -0.9 88,168 - GFF70 0.84 -1.8 88,168 - GFF70 0.84 -0.5 88,287 + -0.1 88,287 + +2.6 88,288 - +0.5 88,288 - +1.2 88,288 - +0.8 88,446 + +0.1 88,526 - GFF71 0.14 -0.5 88,526 - GFF71 0.14 -0.5 88,795 + GFF71 0.48 -0.6 88,795 + GFF71 0.48 -0.4 88,796 - GFF71 0.48 +0.2 89,232 + -0.2 89,449 + GFF72 0.18 -0.2 89,449 + GFF72 0.18 -0.3 89,449 + GFF72 0.18 -0.8 89,449 + GFF72 0.18 +0.1 89,449 + GFF72 0.18 -0.1 89,449 + GFF72 0.18 -0.1 89,450 - GFF72 0.18 -0.9 89,450 - GFF72 0.18 +0.2 89,450 - GFF72 0.18 +0.2 89,671 + GFF72 0.38 -0.1 89,671 + GFF72 0.38 -0.1 89,671 + GFF72 0.38 -0.4 89,671 + GFF72 0.38 -0.0 89,671 + GFF72 0.38 -0.8 89,672 - GFF72 0.38 -0.4 89,672 - GFF72 0.38 -0.4 89,672 - GFF72 0.38 +0.6 90,134 - GFF72 0.78 +0.2
Or see this region's nucleotide sequence