Strain Fitness in Variovorax sp. SCN45 around GFF5224

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5223 and GFF5224 are separated by 122 nucleotidesGFF5224 and GFF5225 are separated by 106 nucleotidesGFF5225 and GFF5226 overlap by 4 nucleotides GFF5223 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 86,235 to 87,134 GFF5223 GFF5224 - Uncharacterized MFS-type transporter, at 87,257 to 88,528 GFF5224 GFF5225 - hypothetical protein, at 88,635 to 88,823 GFF5225 GFF5226 - Probable cytochrome c2, at 88,820 to 89,536 GFF5226 Position (kb) 87 88 89Strain fitness (log2 ratio) -1 0 1 2at 86.414 kb on - strand, within GFF5223at 86.734 kb on + strand, within GFF5223at 86.735 kb on - strand, within GFF5223at 86.735 kb on - strand, within GFF5223at 86.735 kb on - strand, within GFF5223at 86.941 kb on + strand, within GFF5223at 86.942 kb on - strand, within GFF5223at 86.942 kb on - strand, within GFF5223at 87.219 kb on - strandat 87.219 kb on - strandat 87.362 kb on + strandat 87.362 kb on + strandat 87.362 kb on + strandat 87.363 kb on - strandat 87.363 kb on - strandat 87.434 kb on + strand, within GFF5224at 87.434 kb on + strand, within GFF5224at 87.434 kb on + strand, within GFF5224at 87.435 kb on - strand, within GFF5224at 87.435 kb on - strand, within GFF5224at 87.435 kb on - strand, within GFF5224at 87.716 kb on + strand, within GFF5224at 87.716 kb on + strand, within GFF5224at 87.717 kb on - strand, within GFF5224at 87.785 kb on + strand, within GFF5224at 87.785 kb on + strand, within GFF5224at 87.785 kb on + strand, within GFF5224at 87.785 kb on + strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 87.786 kb on - strand, within GFF5224at 88.022 kb on + strand, within GFF5224at 88.022 kb on + strand, within GFF5224at 88.022 kb on + strand, within GFF5224at 88.220 kb on + strand, within GFF5224at 88.221 kb on - strand, within GFF5224at 88.271 kb on + strand, within GFF5224at 88.386 kb on + strand, within GFF5224at 88.406 kb on + strandat 88.406 kb on + strandat 88.566 kb on - strandat 88.566 kb on - strandat 88.566 kb on - strandat 89.021 kb on + strand, within GFF5226at 89.081 kb on + strand, within GFF5226at 89.081 kb on + strand, within GFF5226at 89.081 kb on + strand, within GFF5226at 89.082 kb on - strand, within GFF5226at 89.082 kb on - strand, within GFF5226at 89.082 kb on - strand, within GFF5226at 89.082 kb on - strand, within GFF5226at 89.082 kb on - strand, within GFF5226at 89.130 kb on - strand, within GFF5226at 89.153 kb on + strand, within GFF5226at 89.153 kb on + strand, within GFF5226at 89.327 kb on + strand, within GFF5226at 89.328 kb on - strand, within GFF5226at 89.328 kb on - strand, within GFF5226

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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86,414 - GFF5223 0.20 -0.0
86,734 + GFF5223 0.55 -0.2
86,735 - GFF5223 0.56 +0.1
86,735 - GFF5223 0.56 +0.6
86,735 - GFF5223 0.56 +0.3
86,941 + GFF5223 0.78 +1.8
86,942 - GFF5223 0.79 -0.1
86,942 - GFF5223 0.79 -0.7
87,219 - +0.6
87,219 - -0.6
87,362 + +0.7
87,362 + -0.3
87,362 + -1.0
87,363 - -0.5
87,363 - +0.3
87,434 + GFF5224 0.14 +0.2
87,434 + GFF5224 0.14 -0.3
87,434 + GFF5224 0.14 -0.0
87,435 - GFF5224 0.14 -0.4
87,435 - GFF5224 0.14 +1.6
87,435 - GFF5224 0.14 -0.0
87,716 + GFF5224 0.36 -1.0
87,716 + GFF5224 0.36 +0.3
87,717 - GFF5224 0.36 -1.0
87,785 + GFF5224 0.42 -0.9
87,785 + GFF5224 0.42 -0.3
87,785 + GFF5224 0.42 +0.6
87,785 + GFF5224 0.42 +0.2
87,786 - GFF5224 0.42 +0.8
87,786 - GFF5224 0.42 +0.6
87,786 - GFF5224 0.42 +0.0
87,786 - GFF5224 0.42 +2.6
87,786 - GFF5224 0.42 +0.4
87,786 - GFF5224 0.42 -0.8
88,022 + GFF5224 0.60 +0.6
88,022 + GFF5224 0.60 -0.0
88,022 + GFF5224 0.60 +0.3
88,220 + GFF5224 0.76 +0.3
88,221 - GFF5224 0.76 -0.4
88,271 + GFF5224 0.80 -0.5
88,386 + GFF5224 0.89 +1.1
88,406 + +0.7
88,406 + -0.7
88,566 - -0.4
88,566 - +0.6
88,566 - +2.0
89,021 + GFF5226 0.28 +1.0
89,081 + GFF5226 0.36 +0.1
89,081 + GFF5226 0.36 -0.1
89,081 + GFF5226 0.36 -0.3
89,082 - GFF5226 0.37 -0.3
89,082 - GFF5226 0.37 -0.0
89,082 - GFF5226 0.37 +1.1
89,082 - GFF5226 0.37 +0.7
89,082 - GFF5226 0.37 -0.3
89,130 - GFF5226 0.43 +1.6
89,153 + GFF5226 0.46 +0.3
89,153 + GFF5226 0.46 -0.3
89,327 + GFF5226 0.71 +0.5
89,328 - GFF5226 0.71 -0.6
89,328 - GFF5226 0.71 -0.5

Or see this region's nucleotide sequence