Strain Fitness in Variovorax sp. SCN45 around GFF520

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF518 and GFF519 are separated by 145 nucleotidesGFF519 and GFF520 overlap by 4 nucleotidesGFF520 and GFF521 overlap by 4 nucleotides GFF518 - Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains, at 556,972 to 558,504 GFF518 GFF519 - Transcriptional regulator, AcrR family, at 558,650 to 559,345 GFF519 GFF520 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 559,342 to 560,868 GFF520 GFF521 - Secretion protein HlyD precursor, at 560,865 to 562,001 GFF521 Position (kb) 559 560 561Strain fitness (log2 ratio) -1 0 1 2at 558.364 kb on + strandat 558.364 kb on + strandat 558.364 kb on + strandat 558.364 kb on + strandat 558.365 kb on - strandat 558.365 kb on - strandat 558.515 kb on + strandat 558.516 kb on - strandat 558.516 kb on - strandat 558.582 kb on - strandat 558.606 kb on - strandat 558.606 kb on - strandat 558.606 kb on - strandat 558.639 kb on + strandat 558.639 kb on + strandat 558.827 kb on + strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.828 kb on - strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.830 kb on + strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.831 kb on - strand, within GFF519at 558.855 kb on - strand, within GFF519at 558.949 kb on + strand, within GFF519at 558.949 kb on + strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 558.950 kb on - strand, within GFF519at 559.190 kb on + strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.191 kb on - strand, within GFF519at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.734 kb on + strand, within GFF520at 559.735 kb on - strand, within GFF520at 559.735 kb on - strand, within GFF520at 559.918 kb on + strand, within GFF520at 559.918 kb on + strand, within GFF520at 560.260 kb on + strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.261 kb on - strand, within GFF520at 560.366 kb on - strand, within GFF520at 560.485 kb on + strand, within GFF520at 560.486 kb on - strand, within GFF520at 560.486 kb on - strand, within GFF520at 560.506 kb on + strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.507 kb on - strand, within GFF520at 560.611 kb on + strand, within GFF520at 560.611 kb on + strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.612 kb on - strand, within GFF520at 560.644 kb on + strand, within GFF520at 560.645 kb on - strand, within GFF520at 560.761 kb on + strandat 561.163 kb on + strand, within GFF521at 561.163 kb on + strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.164 kb on - strand, within GFF521at 561.324 kb on + strand, within GFF521at 561.325 kb on - strand, within GFF521at 561.325 kb on - strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.669 kb on + strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521at 561.670 kb on - strand, within GFF521

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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558,364 + +0.4
558,364 + +0.4
558,364 + -0.6
558,364 + -0.2
558,365 - -0.1
558,365 - -0.9
558,515 + +0.1
558,516 - +0.4
558,516 - -0.6
558,582 - +0.9
558,606 - -0.8
558,606 - -0.3
558,606 - -0.6
558,639 + +0.3
558,639 + -0.1
558,827 + GFF519 0.25 +0.5
558,828 - GFF519 0.26 -0.6
558,828 - GFF519 0.26 +0.2
558,828 - GFF519 0.26 -0.3
558,830 + GFF519 0.26 -0.0
558,830 + GFF519 0.26 -0.5
558,830 + GFF519 0.26 +0.6
558,830 + GFF519 0.26 +0.5
558,830 + GFF519 0.26 -0.2
558,831 - GFF519 0.26 -0.2
558,831 - GFF519 0.26 -1.2
558,831 - GFF519 0.26 -0.8
558,831 - GFF519 0.26 +0.3
558,855 - GFF519 0.29 +0.7
558,949 + GFF519 0.43 -0.8
558,949 + GFF519 0.43 -0.2
558,950 - GFF519 0.43 -0.4
558,950 - GFF519 0.43 +0.2
558,950 - GFF519 0.43 -0.9
558,950 - GFF519 0.43 -0.8
558,950 - GFF519 0.43 +0.6
559,190 + GFF519 0.78 +1.2
559,191 - GFF519 0.78 +0.9
559,191 - GFF519 0.78 -0.1
559,191 - GFF519 0.78 -0.3
559,191 - GFF519 0.78 -0.3
559,191 - GFF519 0.78 +0.2
559,191 - GFF519 0.78 -0.8
559,191 - GFF519 0.78 +0.0
559,734 + GFF520 0.26 -0.2
559,734 + GFF520 0.26 -0.2
559,734 + GFF520 0.26 -0.4
559,734 + GFF520 0.26 -0.8
559,735 - GFF520 0.26 +0.2
559,735 - GFF520 0.26 -0.7
559,918 + GFF520 0.38 +0.6
559,918 + GFF520 0.38 +0.0
560,260 + GFF520 0.60 -0.0
560,261 - GFF520 0.60 -0.9
560,261 - GFF520 0.60 +0.1
560,261 - GFF520 0.60 +0.8
560,261 - GFF520 0.60 +0.2
560,261 - GFF520 0.60 +1.2
560,366 - GFF520 0.67 -0.2
560,485 + GFF520 0.75 +0.1
560,486 - GFF520 0.75 +0.2
560,486 - GFF520 0.75 +1.2
560,506 + GFF520 0.76 +0.1
560,507 - GFF520 0.76 -1.5
560,507 - GFF520 0.76 -0.1
560,507 - GFF520 0.76 +0.1
560,611 + GFF520 0.83 +0.4
560,611 + GFF520 0.83 -0.4
560,612 - GFF520 0.83 -0.1
560,612 - GFF520 0.83 +0.6
560,612 - GFF520 0.83 +0.7
560,612 - GFF520 0.83 -0.2
560,644 + GFF520 0.85 +0.2
560,645 - GFF520 0.85 +1.0
560,761 + +0.6
561,163 + GFF521 0.26 +0.1
561,163 + GFF521 0.26 -0.0
561,164 - GFF521 0.26 +0.1
561,164 - GFF521 0.26 +0.6
561,164 - GFF521 0.26 +0.2
561,324 + GFF521 0.40 -0.5
561,325 - GFF521 0.40 +2.2
561,325 - GFF521 0.40 +0.4
561,669 + GFF521 0.71 +0.4
561,669 + GFF521 0.71 -0.6
561,669 + GFF521 0.71 -0.2
561,669 + GFF521 0.71 -0.8
561,669 + GFF521 0.71 +0.8
561,669 + GFF521 0.71 +0.3
561,670 - GFF521 0.71 +0.2
561,670 - GFF521 0.71 +0.4
561,670 - GFF521 0.71 +1.2
561,670 - GFF521 0.71 -0.1
561,670 - GFF521 0.71 +0.2
561,670 - GFF521 0.71 +0.5
561,670 - GFF521 0.71 -0.3

Or see this region's nucleotide sequence