Strain Fitness in Variovorax sp. SCN45 around GFF3378

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3376 and GFF3377 are separated by 58 nucleotidesGFF3377 and GFF3378 are separated by 79 nucleotidesGFF3378 and GFF3379 are separated by 6 nucleotides GFF3376 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 195,787 to 197,343 GFF3376 GFF3377 - Enoyl-CoA hydratase (EC 4.2.1.17) @ Enoyl-CoA hydratase EchA5 (EC 4.2.1.17), at 197,402 to 198,184 GFF3377 GFF3378 - hypothetical protein, at 198,264 to 199,076 GFF3378 GFF3379 - Aromatic--CoA ligase, at 199,083 to 200,894 GFF3379 Position (kb) 198 199 200Strain fitness (log2 ratio) -2 -1 0 1at 197.297 kb on + strandat 197.297 kb on + strandat 197.297 kb on + strandat 197.298 kb on - strandat 197.865 kb on + strand, within GFF3377at 198.172 kb on - strandat 198.172 kb on - strandat 198.172 kb on - strandat 198.238 kb on - strandat 198.256 kb on - strandat 198.274 kb on + strandat 198.274 kb on + strandat 198.274 kb on + strandat 198.274 kb on + strandat 198.275 kb on - strandat 198.275 kb on - strandat 198.275 kb on - strandat 198.275 kb on - strandat 198.278 kb on + strandat 198.278 kb on + strandat 198.278 kb on + strandat 198.279 kb on - strandat 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.468 kb on + strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 198.469 kb on - strand, within GFF3378at 199.183 kb on + strandat 199.183 kb on + strandat 199.183 kb on + strandat 199.184 kb on - strandat 199.184 kb on - strandat 199.184 kb on - strandat 199.184 kb on - strandat 199.184 kb on - strandat 199.567 kb on + strand, within GFF3379at 199.570 kb on + strand, within GFF3379at 199.571 kb on - strand, within GFF3379at 199.874 kb on - strand, within GFF3379at 199.933 kb on + strand, within GFF3379at 200.050 kb on + strand, within GFF3379at 200.050 kb on + strand, within GFF3379at 200.051 kb on - strand, within GFF3379

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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197,297 + -0.1
197,297 + -2.2
197,297 + +1.7
197,298 - -0.4
197,865 + GFF3377 0.59 -0.1
198,172 - +0.2
198,172 - +1.0
198,172 - -0.9
198,238 - -1.1
198,256 - +0.4
198,274 + -1.1
198,274 + -1.3
198,274 + +0.7
198,274 + -0.3
198,275 - -2.0
198,275 - -0.5
198,275 - +0.1
198,275 - +0.0
198,278 + -0.2
198,278 + +0.2
198,278 + -0.0
198,279 - -0.9
198,468 + GFF3378 0.25 -0.2
198,468 + GFF3378 0.25 -0.4
198,468 + GFF3378 0.25 +1.3
198,468 + GFF3378 0.25 +0.3
198,468 + GFF3378 0.25 -0.2
198,468 + GFF3378 0.25 -0.2
198,468 + GFF3378 0.25 -0.0
198,468 + GFF3378 0.25 +0.2
198,469 - GFF3378 0.25 +1.0
198,469 - GFF3378 0.25 +0.4
198,469 - GFF3378 0.25 +0.0
198,469 - GFF3378 0.25 +0.0
198,469 - GFF3378 0.25 -0.4
198,469 - GFF3378 0.25 +0.3
198,469 - GFF3378 0.25 +1.2
198,469 - GFF3378 0.25 -1.3
198,469 - GFF3378 0.25 -0.2
199,183 + -1.0
199,183 + -1.0
199,183 + -0.4
199,184 - +0.8
199,184 - +0.8
199,184 - -0.6
199,184 - -0.2
199,184 - -0.4
199,567 + GFF3379 0.27 +0.5
199,570 + GFF3379 0.27 -1.1
199,571 - GFF3379 0.27 +1.3
199,874 - GFF3379 0.44 -0.2
199,933 + GFF3379 0.47 -0.3
200,050 + GFF3379 0.53 +0.0
200,050 + GFF3379 0.53 -0.6
200,051 - GFF3379 0.53 +1.6

Or see this region's nucleotide sequence