Strain Fitness in Variovorax sp. SCN45 around GFF3324

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3322 and GFF3323 are separated by 181 nucleotidesGFF3323 and GFF3324 are separated by 7 nucleotidesGFF3324 and GFF3325 are separated by 17 nucleotidesGFF3325 and GFF3326 are separated by 8 nucleotides GFF3322 - hypothetical protein, at 148,892 to 149,752 GFF3322 GFF3323 - Tricarboxylate transport transcriptional regulator TctD, at 149,934 to 150,578 GFF3323 GFF3324 - hypothetical protein, at 150,586 to 151,980 GFF3324 GFF3325 - Uncharacterized protein YaeR with similarity to glyoxylase family, at 151,998 to 152,753 GFF3325 GFF3326 - hypothetical protein, at 152,762 to 153,223 GFF3326 Position (kb) 150 151 152Strain fitness (log2 ratio) -1 0 1 2at 149.613 kb on + strand, within GFF3322at 149.789 kb on + strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.790 kb on - strandat 149.800 kb on + strandat 149.800 kb on + strandat 149.800 kb on + strandat 149.801 kb on - strandat 149.801 kb on - strandat 149.801 kb on - strandat 149.868 kb on + strandat 150.555 kb on + strandat 150.555 kb on + strandat 150.725 kb on - strandat 150.770 kb on - strand, within GFF3324at 150.871 kb on + strand, within GFF3324at 150.872 kb on - strand, within GFF3324at 150.976 kb on + strand, within GFF3324at 150.976 kb on + strand, within GFF3324at 150.976 kb on + strand, within GFF3324at 150.977 kb on - strand, within GFF3324at 151.106 kb on - strand, within GFF3324at 151.430 kb on + strand, within GFF3324at 151.430 kb on + strand, within GFF3324at 151.430 kb on + strand, within GFF3324at 151.431 kb on - strand, within GFF3324at 151.431 kb on - strand, within GFF3324at 151.431 kb on - strand, within GFF3324at 151.431 kb on - strand, within GFF3324at 151.431 kb on - strand, within GFF3324at 151.657 kb on + strand, within GFF3324at 151.658 kb on - strand, within GFF3324at 151.658 kb on - strand, within GFF3324at 151.658 kb on - strand, within GFF3324at 151.717 kb on + strand, within GFF3324at 151.732 kb on - strand, within GFF3324at 152.122 kb on + strand, within GFF3325at 152.123 kb on - strand, within GFF3325at 152.232 kb on + strand, within GFF3325at 152.233 kb on - strand, within GFF3325at 152.377 kb on + strand, within GFF3325at 152.378 kb on - strand, within GFF3325at 152.378 kb on - strand, within GFF3325at 152.378 kb on - strand, within GFF3325at 152.434 kb on + strand, within GFF3325at 152.434 kb on + strand, within GFF3325at 152.434 kb on + strand, within GFF3325at 152.435 kb on - strand, within GFF3325at 152.686 kb on + strandat 152.687 kb on - strandat 152.687 kb on - strandat 152.708 kb on - strandat 152.820 kb on + strand, within GFF3326at 152.820 kb on + strand, within GFF3326at 152.820 kb on + strand, within GFF3326at 152.821 kb on - strand, within GFF3326at 152.821 kb on - strand, within GFF3326at 152.886 kb on + strand, within GFF3326at 152.887 kb on - strand, within GFF3326at 152.887 kb on - strand, within GFF3326at 152.887 kb on - strand, within GFF3326

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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149,613 + GFF3322 0.84 -0.6
149,789 + +0.4
149,790 - -0.3
149,790 - +0.3
149,790 - -0.2
149,790 - -0.4
149,790 - -0.8
149,790 - -0.8
149,790 - +0.5
149,800 + -0.0
149,800 + -1.5
149,800 + +1.3
149,801 - -0.2
149,801 - -0.6
149,801 - +0.4
149,868 + -0.2
150,555 + -0.7
150,555 + +1.2
150,725 - -0.7
150,770 - GFF3324 0.13 +0.3
150,871 + GFF3324 0.20 +0.3
150,872 - GFF3324 0.21 -0.0
150,976 + GFF3324 0.28 -1.2
150,976 + GFF3324 0.28 -0.7
150,976 + GFF3324 0.28 +0.2
150,977 - GFF3324 0.28 +0.3
151,106 - GFF3324 0.37 -0.4
151,430 + GFF3324 0.61 -0.5
151,430 + GFF3324 0.61 -0.2
151,430 + GFF3324 0.61 -0.6
151,431 - GFF3324 0.61 -0.3
151,431 - GFF3324 0.61 -0.7
151,431 - GFF3324 0.61 -0.1
151,431 - GFF3324 0.61 -0.7
151,431 - GFF3324 0.61 +0.4
151,657 + GFF3324 0.77 +0.3
151,658 - GFF3324 0.77 -0.7
151,658 - GFF3324 0.77 -0.4
151,658 - GFF3324 0.77 -1.3
151,717 + GFF3324 0.81 +1.8
151,732 - GFF3324 0.82 -0.9
152,122 + GFF3325 0.16 +0.2
152,123 - GFF3325 0.17 +0.1
152,232 + GFF3325 0.31 +0.5
152,233 - GFF3325 0.31 +0.4
152,377 + GFF3325 0.50 -0.7
152,378 - GFF3325 0.50 +1.0
152,378 - GFF3325 0.50 -1.6
152,378 - GFF3325 0.50 -1.6
152,434 + GFF3325 0.58 -0.8
152,434 + GFF3325 0.58 +0.2
152,434 + GFF3325 0.58 +0.1
152,435 - GFF3325 0.58 -0.4
152,686 + +0.4
152,687 - -0.1
152,687 - +0.5
152,708 - +0.3
152,820 + GFF3326 0.13 -0.4
152,820 + GFF3326 0.13 +0.4
152,820 + GFF3326 0.13 -0.3
152,821 - GFF3326 0.13 +0.7
152,821 - GFF3326 0.13 +0.8
152,886 + GFF3326 0.27 -0.6
152,887 - GFF3326 0.27 +0.1
152,887 - GFF3326 0.27 -0.2
152,887 - GFF3326 0.27 +0.0

Or see this region's nucleotide sequence