Strain Fitness in Variovorax sp. SCN45 around GFF3169

Experiment: no phage control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3168 and GFF3169 are separated by 10 nucleotidesGFF3169 and GFF3170 overlap by 4 nucleotidesGFF3170 and GFF3171 are separated by 10 nucleotides GFF3168 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 607,781 to 609,223 GFF3168 GFF3169 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 609,234 to 610,754 GFF3169 GFF3170 - hypothetical protein, at 610,751 to 611,548 GFF3170 GFF3171 - Uncharacterized protein conserved in bacteria, at 611,559 to 612,785 GFF3171 Position (kb) 609 610 611Strain fitness (log2 ratio) -1 0 1 2at 608.400 kb on - strand, within GFF3168at 608.904 kb on - strand, within GFF3168at 608.909 kb on + strand, within GFF3168at 609.528 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.729 kb on + strand, within GFF3169at 609.730 kb on - strand, within GFF3169at 609.730 kb on - strand, within GFF3169at 609.799 kb on - strand, within GFF3169at 609.799 kb on - strand, within GFF3169at 609.861 kb on + strand, within GFF3169at 609.862 kb on - strand, within GFF3169at 610.060 kb on + strand, within GFF3169at 610.061 kb on - strand, within GFF3169at 610.561 kb on - strand, within GFF3169at 610.656 kb on + strandat 610.802 kb on + strandat 610.803 kb on - strandat 610.803 kb on - strandat 610.804 kb on + strandat 610.805 kb on - strandat 611.561 kb on - strandat 611.603 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no phage control
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608,400 - GFF3168 0.43 -0.4
608,904 - GFF3168 0.78 +0.9
608,909 + GFF3168 0.78 +0.2
609,528 + GFF3169 0.19 +0.5
609,729 + GFF3169 0.33 +0.9
609,729 + GFF3169 0.33 +1.0
609,729 + GFF3169 0.33 +0.1
609,730 - GFF3169 0.33 -0.4
609,730 - GFF3169 0.33 -0.6
609,799 - GFF3169 0.37 +0.5
609,799 - GFF3169 0.37 +0.1
609,861 + GFF3169 0.41 -0.3
609,862 - GFF3169 0.41 +2.5
610,060 + GFF3169 0.54 -0.3
610,061 - GFF3169 0.54 -0.5
610,561 - GFF3169 0.87 +0.1
610,656 + +0.1
610,802 + -1.5
610,803 - -0.2
610,803 - -1.4
610,804 + +0.8
610,805 - -1.6
611,561 - +0.8
611,603 - +0.0

Or see this region's nucleotide sequence