Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3168 and GFF3169 are separated by 10 nucleotides GFF3169 and GFF3170 overlap by 4 nucleotides GFF3170 and GFF3171 are separated by 10 nucleotides
GFF3168 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 607,781 to 609,223
GFF3168
GFF3169 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 609,234 to 610,754
GFF3169
GFF3170 - hypothetical protein, at 610,751 to 611,548
GFF3170
GFF3171 - Uncharacterized protein conserved in bacteria, at 611,559 to 612,785
GFF3171
Position (kb)
609
610
611 Strain fitness (log2 ratio)
-1
0
1
2 at 608.400 kb on - strand, within GFF3168 at 608.904 kb on - strand, within GFF3168 at 608.909 kb on + strand, within GFF3168 at 609.528 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.729 kb on + strand, within GFF3169 at 609.730 kb on - strand, within GFF3169 at 609.730 kb on - strand, within GFF3169 at 609.799 kb on - strand, within GFF3169 at 609.799 kb on - strand, within GFF3169 at 609.861 kb on + strand, within GFF3169 at 609.862 kb on - strand, within GFF3169 at 610.060 kb on + strand, within GFF3169 at 610.061 kb on - strand, within GFF3169 at 610.561 kb on - strand, within GFF3169 at 610.656 kb on + strand at 610.802 kb on + strand at 610.803 kb on - strand at 610.803 kb on - strand at 610.804 kb on + strand at 610.805 kb on - strand at 611.561 kb on - strand at 611.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 608,400 - GFF3168 0.43 -0.4 608,904 - GFF3168 0.78 +0.9 608,909 + GFF3168 0.78 +0.2 609,528 + GFF3169 0.19 +0.5 609,729 + GFF3169 0.33 +0.9 609,729 + GFF3169 0.33 +1.0 609,729 + GFF3169 0.33 +0.1 609,730 - GFF3169 0.33 -0.4 609,730 - GFF3169 0.33 -0.6 609,799 - GFF3169 0.37 +0.5 609,799 - GFF3169 0.37 +0.1 609,861 + GFF3169 0.41 -0.3 609,862 - GFF3169 0.41 +2.5 610,060 + GFF3169 0.54 -0.3 610,061 - GFF3169 0.54 -0.5 610,561 - GFF3169 0.87 +0.1 610,656 + +0.1 610,802 + -1.5 610,803 - -0.2 610,803 - -1.4 610,804 + +0.8 610,805 - -1.6 611,561 - +0.8 611,603 - +0.0
Or see this region's nucleotide sequence