Experiment: phage_bap4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1133 and GFF1134 are separated by 17 nucleotides GFF1134 and GFF1135 overlap by 14 nucleotides GFF1135 and GFF1136 are separated by 235 nucleotides GFF1136 and GFF1137 are separated by 4 nucleotides GFF1137 and GFF1138 are separated by 129 nucleotides
GFF1133 - FIG00431745: hypothetical protein, at 450,305 to 451,222
GFF1133
GFF1134 - FIG00931167: hypothetical protein, at 451,240 to 451,611
GFF1134
GFF1135 - hypothetical protein, at 451,598 to 451,828
GFF1135
GFF1136 - hypothetical protein, at 452,064 to 452,651
GFF1136
GFF1137 - FIG00348850: hypothetical protein, at 452,656 to 453,498
GFF1137
GFF1138 - Transcriptional regulator, AcrR family, at 453,628 to 454,257
GFF1138
Position (kb) 452
453 Strain fitness (log2 ratio) -2
-1
0
1
2 at 451.203 kb on - strand at 451.203 kb on - strand at 451.203 kb on - strand at 451.203 kb on - strand at 451.203 kb on - strand at 451.220 kb on + strand at 451.220 kb on + strand at 451.220 kb on + strand at 451.220 kb on + strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.221 kb on - strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.226 kb on + strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.227 kb on - strand at 451.360 kb on + strand, within GFF1134 at 451.360 kb on + strand, within GFF1134 at 451.361 kb on - strand, within GFF1134 at 451.361 kb on - strand, within GFF1134 at 451.361 kb on - strand, within GFF1134 at 451.441 kb on + strand, within GFF1134 at 451.462 kb on + strand, within GFF1134 at 451.462 kb on + strand, within GFF1134 at 451.463 kb on - strand, within GFF1134 at 451.463 kb on - strand, within GFF1134 at 451.463 kb on - strand, within GFF1134 at 451.463 kb on - strand, within GFF1134 at 451.470 kb on + strand, within GFF1134 at 451.471 kb on - strand, within GFF1134 at 451.471 kb on - strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.477 kb on + strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.478 kb on - strand, within GFF1134 at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.614 kb on + strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.615 kb on - strand at 451.646 kb on + strand, within GFF1135 at 451.646 kb on + strand, within GFF1135 at 451.646 kb on + strand, within GFF1135 at 451.646 kb on + strand, within GFF1135 at 451.647 kb on - strand, within GFF1135 at 451.647 kb on - strand, within GFF1135 at 451.809 kb on + strand at 451.810 kb on - strand at 451.826 kb on + strand at 451.826 kb on + strand at 451.826 kb on + strand at 451.826 kb on + strand at 451.826 kb on + strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.827 kb on - strand at 451.848 kb on + strand at 451.928 kb on - strand at 451.929 kb on + strand at 451.930 kb on - strand at 451.930 kb on - strand at 451.930 kb on - strand at 451.930 kb on - strand at 451.930 kb on - strand at 451.930 kb on - strand at 451.945 kb on + strand at 451.945 kb on + strand at 451.945 kb on + strand at 451.946 kb on - strand at 451.954 kb on + strand at 451.954 kb on + strand at 451.954 kb on + strand at 451.955 kb on - strand at 451.955 kb on - strand at 451.955 kb on - strand at 451.964 kb on + strand at 451.964 kb on - strand at 451.965 kb on - strand at 451.977 kb on + strand at 452.040 kb on - strand at 452.069 kb on + strand at 452.069 kb on + strand at 452.069 kb on + strand at 452.070 kb on - strand at 452.070 kb on - strand at 452.070 kb on - strand at 452.070 kb on - strand at 452.197 kb on + strand, within GFF1136 at 452.197 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.388 kb on + strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.389 kb on - strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.399 kb on + strand, within GFF1136 at 452.400 kb on - strand, within GFF1136 at 452.400 kb on - strand, within GFF1136 at 452.400 kb on - strand, within GFF1136 at 452.400 kb on - strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.451 kb on + strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.452 kb on - strand, within GFF1136 at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.699 kb on + strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.700 kb on - strand at 452.761 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.777 kb on + strand, within GFF1137 at 452.778 kb on - strand, within GFF1137 at 452.778 kb on - strand, within GFF1137 at 452.778 kb on - strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.984 kb on + strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 452.985 kb on - strand, within GFF1137 at 453.305 kb on + strand, within GFF1137 at 453.503 kb on + strand at 453.518 kb on + strand at 453.518 kb on + strand at 453.546 kb on + strand at 453.547 kb on - strand at 453.547 kb on - strand at 453.547 kb on - strand at 453.547 kb on - strand at 453.547 kb on - strand at 453.547 kb on - strand at 453.600 kb on + strand at 453.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phage_bap4 remove 451,203 - -0.9 451,203 - -1.0 451,203 - +0.1 451,203 - -0.6 451,203 - -0.5 451,220 + +0.2 451,220 + -0.2 451,220 + -0.5 451,220 + -0.3 451,221 - -1.9 451,221 - -0.6 451,221 - +0.1 451,221 - +0.2 451,221 - -0.1 451,221 - +0.7 451,221 - +1.4 451,221 - -0.3 451,226 + -0.9 451,226 + +0.4 451,226 + +0.5 451,226 + -0.5 451,226 + -0.0 451,226 + -0.1 451,226 + -0.5 451,226 + +0.1 451,226 + -0.5 451,226 + -0.2 451,226 + -0.2 451,226 + +0.1 451,226 + -0.3 451,226 + +0.4 451,226 + -0.1 451,226 + +0.9 451,226 + +2.0 451,226 + -1.2 451,226 + -0.3 451,226 + -0.1 451,226 + +0.0 451,226 + -0.4 451,226 + -0.4 451,226 + -0.8 451,227 - +0.6 451,227 - -1.2 451,227 - +0.2 451,227 - +1.4 451,227 - +0.6 451,227 - +1.1 451,227 - +0.1 451,227 - +0.5 451,227 - -1.1 451,227 - +0.2 451,227 - +0.2 451,227 - -0.4 451,227 - -0.0 451,227 - +0.6 451,227 - -0.6 451,227 - +0.9 451,227 - +0.7 451,227 - +0.1 451,227 - -0.2 451,227 - +1.2 451,227 - -0.5 451,227 - -0.5 451,360 + GFF1134 0.32 -1.7 451,360 + GFF1134 0.32 -1.2 451,361 - GFF1134 0.33 -0.3 451,361 - GFF1134 0.33 +0.2 451,361 - GFF1134 0.33 +0.5 451,441 + GFF1134 0.54 -0.4 451,462 + GFF1134 0.60 -0.3 451,462 + GFF1134 0.60 +0.2 451,463 - GFF1134 0.60 -0.2 451,463 - GFF1134 0.60 -0.6 451,463 - GFF1134 0.60 -0.6 451,463 - GFF1134 0.60 -0.2 451,470 + GFF1134 0.62 +0.4 451,471 - GFF1134 0.62 -0.1 451,471 - GFF1134 0.62 -0.4 451,477 + GFF1134 0.64 -0.2 451,477 + GFF1134 0.64 +0.3 451,477 + GFF1134 0.64 +0.2 451,477 + GFF1134 0.64 +0.2 451,477 + GFF1134 0.64 -0.9 451,477 + GFF1134 0.64 +0.8 451,477 + GFF1134 0.64 +0.4 451,477 + GFF1134 0.64 -0.6 451,477 + GFF1134 0.64 -1.8 451,477 + GFF1134 0.64 -1.4 451,477 + GFF1134 0.64 +0.4 451,477 + GFF1134 0.64 +0.3 451,477 + GFF1134 0.64 -1.1 451,478 - GFF1134 0.64 +0.6 451,478 - GFF1134 0.64 -0.4 451,478 - GFF1134 0.64 +0.2 451,478 - GFF1134 0.64 +0.2 451,478 - GFF1134 0.64 -0.5 451,478 - GFF1134 0.64 +1.0 451,478 - GFF1134 0.64 -0.9 451,478 - GFF1134 0.64 +0.4 451,478 - GFF1134 0.64 +0.0 451,478 - GFF1134 0.64 +0.6 451,478 - GFF1134 0.64 -0.2 451,614 + -0.3 451,614 + +0.0 451,614 + -1.2 451,614 + -1.2 451,614 + +0.8 451,614 + +0.3 451,614 + -0.4 451,614 + -0.2 451,614 + +0.4 451,614 + +0.2 451,614 + -0.4 451,614 + -0.9 451,614 + +0.1 451,614 + +0.2 451,615 - -1.5 451,615 - -0.2 451,615 - -0.3 451,615 - -0.2 451,615 - -0.1 451,615 - -0.7 451,615 - -0.4 451,615 - +0.4 451,646 + GFF1135 0.21 -0.2 451,646 + GFF1135 0.21 -1.2 451,646 + GFF1135 0.21 -0.3 451,646 + GFF1135 0.21 -0.6 451,647 - GFF1135 0.21 -0.4 451,647 - GFF1135 0.21 -0.5 451,809 + -0.2 451,810 - +0.3 451,826 + -0.4 451,826 + -0.7 451,826 + -1.6 451,826 + +1.4 451,826 + -1.1 451,827 - +0.8 451,827 - +1.5 451,827 - +0.0 451,827 - -1.0 451,827 - -0.5 451,827 - +0.7 451,827 - +1.0 451,827 - -1.2 451,827 - +0.3 451,848 + -0.0 451,928 - -2.2 451,929 + -0.6 451,930 - -2.1 451,930 - -1.0 451,930 - +1.4 451,930 - -0.6 451,930 - -0.2 451,930 - -0.7 451,945 + -0.5 451,945 + -0.0 451,945 + -0.5 451,946 - +1.0 451,954 + -0.5 451,954 + -0.4 451,954 + +0.4 451,955 - +1.0 451,955 - -0.5 451,955 - -1.6 451,964 + +0.4 451,964 - +0.4 451,965 - +0.2 451,977 + -0.4 452,040 - -1.1 452,069 + -0.7 452,069 + +0.1 452,069 + -0.2 452,070 - -1.3 452,070 - -0.4 452,070 - +0.8 452,070 - -0.9 452,197 + GFF1136 0.23 -0.2 452,197 + GFF1136 0.23 -0.0 452,388 + GFF1136 0.55 -1.0 452,388 + GFF1136 0.55 +0.1 452,388 + GFF1136 0.55 +0.2 452,388 + GFF1136 0.55 +0.0 452,388 + GFF1136 0.55 -0.6 452,388 + GFF1136 0.55 -0.7 452,388 + GFF1136 0.55 +0.5 452,388 + GFF1136 0.55 +0.1 452,389 - GFF1136 0.55 +0.6 452,389 - GFF1136 0.55 +0.1 452,389 - GFF1136 0.55 -0.4 452,389 - GFF1136 0.55 -0.5 452,389 - GFF1136 0.55 -0.4 452,389 - GFF1136 0.55 +0.2 452,389 - GFF1136 0.55 -0.0 452,389 - GFF1136 0.55 -0.3 452,399 + GFF1136 0.57 -0.9 452,399 + GFF1136 0.57 -0.6 452,399 + GFF1136 0.57 -0.4 452,399 + GFF1136 0.57 -0.5 452,399 + GFF1136 0.57 +0.1 452,399 + GFF1136 0.57 +1.6 452,400 - GFF1136 0.57 -0.4 452,400 - GFF1136 0.57 +0.0 452,400 - GFF1136 0.57 -0.5 452,400 - GFF1136 0.57 +0.4 452,451 + GFF1136 0.66 -0.2 452,451 + GFF1136 0.66 +0.6 452,451 + GFF1136 0.66 +0.2 452,451 + GFF1136 0.66 -0.3 452,451 + GFF1136 0.66 +1.4 452,451 + GFF1136 0.66 -0.4 452,451 + GFF1136 0.66 +0.3 452,451 + GFF1136 0.66 -0.0 452,451 + GFF1136 0.66 +0.9 452,451 + GFF1136 0.66 +0.8 452,451 + GFF1136 0.66 -0.8 452,452 - GFF1136 0.66 -0.3 452,452 - GFF1136 0.66 +0.2 452,452 - GFF1136 0.66 -0.5 452,452 - GFF1136 0.66 -0.9 452,452 - GFF1136 0.66 +0.1 452,452 - GFF1136 0.66 -0.3 452,452 - GFF1136 0.66 -1.2 452,452 - GFF1136 0.66 +1.7 452,452 - GFF1136 0.66 -0.0 452,452 - GFF1136 0.66 -0.5 452,452 - GFF1136 0.66 -0.8 452,452 - GFF1136 0.66 +0.1 452,452 - GFF1136 0.66 +0.0 452,452 - GFF1136 0.66 +0.1 452,452 - GFF1136 0.66 -0.3 452,452 - GFF1136 0.66 -1.9 452,452 - GFF1136 0.66 -1.4 452,699 + -0.3 452,699 + -0.2 452,699 + -0.0 452,699 + +0.5 452,699 + -0.7 452,699 + +0.7 452,699 + -0.9 452,699 + -1.0 452,700 - -0.3 452,700 - -0.8 452,700 - -1.4 452,700 - -0.8 452,700 - -0.4 452,700 - -1.8 452,700 - -0.1 452,700 - -1.8 452,761 + GFF1137 0.12 -0.1 452,777 + GFF1137 0.14 +0.4 452,777 + GFF1137 0.14 +0.2 452,777 + GFF1137 0.14 -0.3 452,777 + GFF1137 0.14 -2.6 452,777 + GFF1137 0.14 -1.1 452,777 + GFF1137 0.14 -0.3 452,778 - GFF1137 0.14 +0.1 452,778 - GFF1137 0.14 -0.5 452,778 - GFF1137 0.14 +0.5 452,984 + GFF1137 0.39 -0.2 452,984 + GFF1137 0.39 +0.0 452,984 + GFF1137 0.39 -0.9 452,984 + GFF1137 0.39 -1.3 452,984 + GFF1137 0.39 -0.7 452,984 + GFF1137 0.39 +0.5 452,984 + GFF1137 0.39 +0.5 452,984 + GFF1137 0.39 +0.1 452,984 + GFF1137 0.39 +0.8 452,984 + GFF1137 0.39 -0.8 452,984 + GFF1137 0.39 -0.7 452,984 + GFF1137 0.39 +1.0 452,984 + GFF1137 0.39 +0.2 452,984 + GFF1137 0.39 -0.4 452,984 + GFF1137 0.39 -1.5 452,984 + GFF1137 0.39 +1.4 452,984 + GFF1137 0.39 -0.2 452,984 + GFF1137 0.39 +0.1 452,984 + GFF1137 0.39 -0.3 452,984 + GFF1137 0.39 -0.3 452,984 + GFF1137 0.39 -0.1 452,984 + GFF1137 0.39 -0.2 452,984 + GFF1137 0.39 -1.6 452,984 + GFF1137 0.39 -1.2 452,985 - GFF1137 0.39 -0.4 452,985 - GFF1137 0.39 +0.2 452,985 - GFF1137 0.39 -0.3 452,985 - GFF1137 0.39 -0.3 452,985 - GFF1137 0.39 -0.4 452,985 - GFF1137 0.39 -0.5 452,985 - GFF1137 0.39 -0.7 452,985 - GFF1137 0.39 -1.3 452,985 - GFF1137 0.39 -0.3 452,985 - GFF1137 0.39 +0.9 452,985 - GFF1137 0.39 +0.3 452,985 - GFF1137 0.39 -1.2 452,985 - GFF1137 0.39 -1.2 452,985 - GFF1137 0.39 -0.9 452,985 - GFF1137 0.39 +0.2 452,985 - GFF1137 0.39 -0.3 452,985 - GFF1137 0.39 -0.9 453,305 + GFF1137 0.77 +0.4 453,503 + -0.2 453,518 + -1.5 453,518 + +0.4 453,546 + -1.4 453,547 - +0.8 453,547 - -0.3 453,547 - -0.3 453,547 - -0.3 453,547 - +0.0 453,547 - -0.3 453,600 + -0.7 453,611 - -0.2
Or see this region's nucleotide sequence