Strain Fitness in Variovorax sp. SCN45 around GFF5743

Experiment: phage_bap4

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5740 and GFF5741 overlap by 4 nucleotidesGFF5741 and GFF5742 overlap by 4 nucleotidesGFF5742 and GFF5743 overlap by 8 nucleotidesGFF5743 and GFF5744 are separated by 27 nucleotidesGFF5744 and GFF5745 are separated by 154 nucleotides GFF5740 - Flagellar biosynthesis protein FliP, at 114,036 to 114,794 GFF5740 GFF5741 - Flagellar biosynthesis protein FliO, at 114,791 to 115,294 GFF5741 GFF5742 - Flagellar motor switch protein FliN, at 115,291 to 115,761 GFF5742 GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758 GFF5743 GFF5744 - Flagellar basal body-associated protein FliL, at 116,786 to 117,289 GFF5744 GFF5745 - Flagellar hook-length control protein FliK, at 117,444 to 118,883 GFF5745 Position (kb) 115 116 117Strain fitness (log2 ratio) -1 0 1 2at 115.355 kb on + strand, within GFF5742at 115.356 kb on - strand, within GFF5742at 115.356 kb on - strand, within GFF5742at 115.397 kb on + strand, within GFF5742at 115.819 kb on - strandat 116.215 kb on - strand, within GFF5743at 116.215 kb on - strand, within GFF5743at 116.340 kb on + strand, within GFF5743at 116.341 kb on - strand, within GFF5743at 116.752 kb on - strandat 117.284 kb on - strandat 117.315 kb on + strandat 117.315 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction phage_bap4
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115,355 + GFF5742 0.14 -0.6
115,356 - GFF5742 0.14 -0.3
115,356 - GFF5742 0.14 -0.1
115,397 + GFF5742 0.23 +0.7
115,819 - +0.8
116,215 - GFF5743 0.46 +1.8
116,215 - GFF5743 0.46 +0.8
116,340 + GFF5743 0.58 +0.0
116,341 - GFF5743 0.58 +0.8
116,752 - +1.8
117,284 - +0.0
117,315 + -0.5
117,315 + -0.3

Or see this region's nucleotide sequence