Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS14615 and A4249_RS14620 are separated by 83 nucleotides A4249_RS14620 and A4249_RS14625 are separated by 199 nucleotides A4249_RS14625 and A4249_RS14630 overlap by 4 nucleotides A4249_RS14630 and A4249_RS14635 are separated by 91 nucleotides
A4249_RS14615: A4249_RS14615 - hydroxymethylglutaryl-CoA lyase, at 2,961,332 to 2,962,246
_RS14615
A4249_RS14620: A4249_RS14620 - hypothetical protein, at 2,962,330 to 2,962,755
_RS14620
A4249_RS14625: A4249_RS14625 - hypothetical protein, at 2,962,955 to 2,963,308
_RS14625
A4249_RS14630: A4249_RS14630 - polyhydroxyalkanoate synthesis repressor PhaR, at 2,963,305 to 2,963,934
_RS14630
A4249_RS14635: A4249_RS14635 - hemolysin III family protein, at 2,964,026 to 2,964,739
_RS14635
Position (kb)
2962
2963
2964 Strain fitness (log2 ratio)
-2
-1
0
1 at 2962.277 kb on + strand at 2962.277 kb on + strand at 2962.278 kb on - strand at 2962.297 kb on + strand at 2962.298 kb on - strand at 2962.372 kb on + strand at 2962.372 kb on + strand at 2962.372 kb on + strand at 2962.372 kb on + strand at 2962.373 kb on - strand, within A4249_RS14620 at 2962.373 kb on - strand, within A4249_RS14620 at 2962.373 kb on - strand, within A4249_RS14620 at 2962.373 kb on - strand, within A4249_RS14620 at 2962.373 kb on - strand, within A4249_RS14620 at 2962.378 kb on - strand, within A4249_RS14620 at 2962.378 kb on - strand, within A4249_RS14620 at 2962.378 kb on - strand, within A4249_RS14620 at 2962.378 kb on - strand, within A4249_RS14620 at 2962.608 kb on - strand, within A4249_RS14620 at 2962.788 kb on + strand at 2962.789 kb on - strand at 2962.810 kb on + strand at 2962.811 kb on - strand at 2963.013 kb on + strand, within A4249_RS14625 at 2963.013 kb on + strand, within A4249_RS14625 at 2963.013 kb on + strand, within A4249_RS14625 at 2963.014 kb on - strand, within A4249_RS14625 at 2963.029 kb on - strand, within A4249_RS14625 at 2963.503 kb on - strand, within A4249_RS14630 at 2963.965 kb on + strand at 2963.965 kb on + strand at 2963.965 kb on + strand at 2964.167 kb on - strand, within A4249_RS14635 at 2964.168 kb on + strand, within A4249_RS14635 at 2964.168 kb on + strand, within A4249_RS14635 at 2964.168 kb on + strand, within A4249_RS14635 at 2964.168 kb on + strand, within A4249_RS14635 at 2964.168 kb on + strand, within A4249_RS14635 at 2964.169 kb on - strand, within A4249_RS14635 at 2964.169 kb on - strand, within A4249_RS14635 at 2964.169 kb on - strand, within A4249_RS14635 at 2964.169 kb on - strand, within A4249_RS14635 at 2964.267 kb on + strand, within A4249_RS14635 at 2964.267 kb on + strand, within A4249_RS14635 at 2964.267 kb on + strand, within A4249_RS14635 at 2964.267 kb on + strand, within A4249_RS14635 at 2964.268 kb on - strand, within A4249_RS14635 at 2964.268 kb on - strand, within A4249_RS14635 at 2964.268 kb on - strand, within A4249_RS14635 at 2964.268 kb on - strand, within A4249_RS14635
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,962,277 + +0.1 2,962,277 + -1.1 2,962,278 - -0.0 2,962,297 + +0.2 2,962,298 - -2.0 2,962,372 + -0.5 2,962,372 + -0.4 2,962,372 + +1.0 2,962,372 + +0.1 2,962,373 - A4249_RS14620 0.10 +0.9 2,962,373 - A4249_RS14620 0.10 +0.0 2,962,373 - A4249_RS14620 0.10 -0.6 2,962,373 - A4249_RS14620 0.10 +1.3 2,962,373 - A4249_RS14620 0.10 +0.8 2,962,378 - A4249_RS14620 0.11 +0.4 2,962,378 - A4249_RS14620 0.11 -0.9 2,962,378 - A4249_RS14620 0.11 +0.5 2,962,378 - A4249_RS14620 0.11 -0.3 2,962,608 - A4249_RS14620 0.65 -1.2 2,962,788 + -0.1 2,962,789 - +0.3 2,962,810 + -1.1 2,962,811 - +0.3 2,963,013 + A4249_RS14625 0.16 +0.7 2,963,013 + A4249_RS14625 0.16 -0.0 2,963,013 + A4249_RS14625 0.16 +0.0 2,963,014 - A4249_RS14625 0.17 -0.5 2,963,029 - A4249_RS14625 0.21 +0.4 2,963,503 - A4249_RS14630 0.31 -1.5 2,963,965 + -0.1 2,963,965 + -0.5 2,963,965 + -1.2 2,964,167 - A4249_RS14635 0.20 +0.3 2,964,168 + A4249_RS14635 0.20 -1.0 2,964,168 + A4249_RS14635 0.20 -0.2 2,964,168 + A4249_RS14635 0.20 -1.1 2,964,168 + A4249_RS14635 0.20 -0.8 2,964,168 + A4249_RS14635 0.20 -0.3 2,964,169 - A4249_RS14635 0.20 -0.1 2,964,169 - A4249_RS14635 0.20 -0.3 2,964,169 - A4249_RS14635 0.20 -0.6 2,964,169 - A4249_RS14635 0.20 +0.1 2,964,267 + A4249_RS14635 0.34 -1.2 2,964,267 + A4249_RS14635 0.34 -0.5 2,964,267 + A4249_RS14635 0.34 -0.0 2,964,267 + A4249_RS14635 0.34 +0.3 2,964,268 - A4249_RS14635 0.34 -0.7 2,964,268 - A4249_RS14635 0.34 -0.1 2,964,268 - A4249_RS14635 0.34 -0.3 2,964,268 - A4249_RS14635 0.34 +0.1
Or see this region's nucleotide sequence