Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02515 and A4249_RS02520 overlap by 1 nucleotides A4249_RS02520 and A4249_RS02525 are separated by 91 nucleotides A4249_RS02525 and A4249_RS02530 are separated by 22 nucleotides
A4249_RS02515: A4249_RS02515 - DNA topoisomerase IB, at 540,202 to 541,224
_RS02515
A4249_RS02520: A4249_RS02520 - hypothetical protein, at 541,224 to 541,568
_RS02520
A4249_RS02525: A4249_RS02525 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 541,660 to 542,475
_RS02525
A4249_RS02530: A4249_RS02530 - GMP synthase, at 542,498 to 543,229
_RS02530
Position (kb)
541
542 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.317 kb on + strand, within A4249_RS02515 at 540.318 kb on - strand, within A4249_RS02515 at 540.318 kb on - strand, within A4249_RS02515 at 540.318 kb on - strand, within A4249_RS02515 at 540.512 kb on + strand, within A4249_RS02515 at 540.512 kb on + strand, within A4249_RS02515 at 540.513 kb on - strand, within A4249_RS02515 at 540.513 kb on - strand, within A4249_RS02515 at 540.513 kb on - strand, within A4249_RS02515 at 540.513 kb on - strand, within A4249_RS02515 at 540.513 kb on - strand, within A4249_RS02515 at 540.566 kb on + strand, within A4249_RS02515 at 540.566 kb on + strand, within A4249_RS02515 at 540.619 kb on + strand, within A4249_RS02515 at 540.619 kb on + strand, within A4249_RS02515 at 540.734 kb on + strand, within A4249_RS02515 at 540.734 kb on + strand, within A4249_RS02515 at 540.735 kb on - strand, within A4249_RS02515 at 540.755 kb on + strand, within A4249_RS02515 at 540.756 kb on - strand, within A4249_RS02515 at 540.953 kb on + strand, within A4249_RS02515 at 540.953 kb on + strand, within A4249_RS02515 at 540.954 kb on - strand, within A4249_RS02515 at 540.993 kb on + strand, within A4249_RS02515 at 541.166 kb on + strand at 541.166 kb on + strand at 541.166 kb on + strand at 541.166 kb on + strand at 541.166 kb on + strand at 541.166 kb on + strand at 541.166 kb on + strand at 541.167 kb on - strand at 541.167 kb on - strand at 541.167 kb on - strand at 541.167 kb on - strand at 541.167 kb on - strand at 541.307 kb on + strand, within A4249_RS02520 at 541.307 kb on + strand, within A4249_RS02520 at 541.308 kb on - strand, within A4249_RS02520 at 541.308 kb on - strand, within A4249_RS02520 at 541.308 kb on - strand, within A4249_RS02520 at 541.309 kb on + strand, within A4249_RS02520 at 541.309 kb on + strand, within A4249_RS02520 at 541.309 kb on + strand, within A4249_RS02520 at 541.309 kb on + strand, within A4249_RS02520 at 541.309 kb on + strand, within A4249_RS02520 at 541.310 kb on - strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.378 kb on + strand, within A4249_RS02520 at 541.379 kb on - strand, within A4249_RS02520 at 541.379 kb on - strand, within A4249_RS02520 at 541.379 kb on - strand, within A4249_RS02520 at 541.379 kb on - strand, within A4249_RS02520 at 541.379 kb on - strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.384 kb on + strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.385 kb on - strand, within A4249_RS02520 at 541.582 kb on - strand at 541.666 kb on + strand at 541.666 kb on + strand at 541.666 kb on + strand at 541.666 kb on + strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.667 kb on - strand at 541.795 kb on + strand, within A4249_RS02525 at 541.795 kb on + strand, within A4249_RS02525 at 541.795 kb on + strand, within A4249_RS02525 at 541.795 kb on + strand, within A4249_RS02525 at 541.795 kb on + strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.796 kb on - strand, within A4249_RS02525 at 541.940 kb on - strand, within A4249_RS02525 at 541.940 kb on - strand, within A4249_RS02525 at 541.940 kb on - strand, within A4249_RS02525 at 541.940 kb on - strand, within A4249_RS02525 at 541.990 kb on + strand, within A4249_RS02525 at 541.990 kb on + strand, within A4249_RS02525 at 541.990 kb on + strand, within A4249_RS02525 at 541.991 kb on - strand, within A4249_RS02525 at 541.991 kb on - strand, within A4249_RS02525 at 542.084 kb on - strand, within A4249_RS02525 at 542.084 kb on - strand, within A4249_RS02525 at 542.084 kb on - strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.164 kb on + strand, within A4249_RS02525 at 542.165 kb on - strand, within A4249_RS02525 at 542.165 kb on - strand, within A4249_RS02525 at 542.165 kb on - strand, within A4249_RS02525 at 542.165 kb on - strand, within A4249_RS02525 at 542.165 kb on - strand, within A4249_RS02525 at 542.486 kb on + strand at 542.486 kb on + strand at 542.486 kb on + strand at 542.486 kb on + strand at 542.487 kb on - strand at 542.487 kb on - strand at 542.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 540,317 + A4249_RS02515 0.11 -0.1 540,317 + A4249_RS02515 0.11 +0.3 540,317 + A4249_RS02515 0.11 -0.1 540,317 + A4249_RS02515 0.11 +0.7 540,317 + A4249_RS02515 0.11 +0.1 540,317 + A4249_RS02515 0.11 -0.8 540,317 + A4249_RS02515 0.11 +0.2 540,317 + A4249_RS02515 0.11 +0.3 540,317 + A4249_RS02515 0.11 -0.3 540,318 - A4249_RS02515 0.11 -0.1 540,318 - A4249_RS02515 0.11 +0.2 540,318 - A4249_RS02515 0.11 +0.1 540,512 + A4249_RS02515 0.30 -0.1 540,512 + A4249_RS02515 0.30 -1.2 540,513 - A4249_RS02515 0.30 -1.9 540,513 - A4249_RS02515 0.30 +1.1 540,513 - A4249_RS02515 0.30 +0.9 540,513 - A4249_RS02515 0.30 +0.0 540,513 - A4249_RS02515 0.30 -2.5 540,566 + A4249_RS02515 0.36 -0.7 540,566 + A4249_RS02515 0.36 -0.9 540,619 + A4249_RS02515 0.41 -1.2 540,619 + A4249_RS02515 0.41 -0.2 540,734 + A4249_RS02515 0.52 -0.3 540,734 + A4249_RS02515 0.52 -0.5 540,735 - A4249_RS02515 0.52 -0.1 540,755 + A4249_RS02515 0.54 -0.2 540,756 - A4249_RS02515 0.54 -1.1 540,953 + A4249_RS02515 0.73 -1.4 540,953 + A4249_RS02515 0.73 -0.4 540,954 - A4249_RS02515 0.74 +0.0 540,993 + A4249_RS02515 0.77 +0.7 541,166 + +1.3 541,166 + +0.5 541,166 + -0.1 541,166 + +0.7 541,166 + -0.5 541,166 + -0.9 541,166 + -0.2 541,167 - -0.0 541,167 - -0.1 541,167 - -0.3 541,167 - -0.0 541,167 - +0.4 541,307 + A4249_RS02520 0.24 +0.1 541,307 + A4249_RS02520 0.24 +0.1 541,308 - A4249_RS02520 0.24 -0.5 541,308 - A4249_RS02520 0.24 +0.2 541,308 - A4249_RS02520 0.24 -0.4 541,309 + A4249_RS02520 0.25 -0.2 541,309 + A4249_RS02520 0.25 +0.2 541,309 + A4249_RS02520 0.25 -0.6 541,309 + A4249_RS02520 0.25 +0.4 541,309 + A4249_RS02520 0.25 -0.9 541,310 - A4249_RS02520 0.25 +0.3 541,378 + A4249_RS02520 0.45 -1.4 541,378 + A4249_RS02520 0.45 -1.2 541,378 + A4249_RS02520 0.45 +0.8 541,378 + A4249_RS02520 0.45 -1.5 541,378 + A4249_RS02520 0.45 +0.3 541,378 + A4249_RS02520 0.45 -0.4 541,378 + A4249_RS02520 0.45 +0.9 541,378 + A4249_RS02520 0.45 -0.5 541,379 - A4249_RS02520 0.45 -0.4 541,379 - A4249_RS02520 0.45 -0.2 541,379 - A4249_RS02520 0.45 +0.7 541,379 - A4249_RS02520 0.45 -0.6 541,379 - A4249_RS02520 0.45 +0.1 541,384 + A4249_RS02520 0.46 -0.1 541,384 + A4249_RS02520 0.46 +0.1 541,384 + A4249_RS02520 0.46 -0.1 541,384 + A4249_RS02520 0.46 -0.1 541,384 + A4249_RS02520 0.46 -0.5 541,384 + A4249_RS02520 0.46 +0.2 541,384 + A4249_RS02520 0.46 +1.3 541,384 + A4249_RS02520 0.46 -0.2 541,384 + A4249_RS02520 0.46 +0.4 541,384 + A4249_RS02520 0.46 -0.6 541,384 + A4249_RS02520 0.46 -0.0 541,384 + A4249_RS02520 0.46 +0.1 541,384 + A4249_RS02520 0.46 -0.1 541,385 - A4249_RS02520 0.47 +0.2 541,385 - A4249_RS02520 0.47 +0.7 541,385 - A4249_RS02520 0.47 -0.6 541,385 - A4249_RS02520 0.47 +0.6 541,385 - A4249_RS02520 0.47 -1.0 541,385 - A4249_RS02520 0.47 +0.2 541,385 - A4249_RS02520 0.47 +0.5 541,385 - A4249_RS02520 0.47 +0.3 541,385 - A4249_RS02520 0.47 +0.8 541,385 - A4249_RS02520 0.47 +0.3 541,385 - A4249_RS02520 0.47 +0.8 541,385 - A4249_RS02520 0.47 -0.8 541,385 - A4249_RS02520 0.47 +0.5 541,385 - A4249_RS02520 0.47 -0.3 541,385 - A4249_RS02520 0.47 -0.3 541,582 - +0.1 541,666 + -0.2 541,666 + -0.5 541,666 + +1.9 541,666 + -0.4 541,667 - +1.0 541,667 - +0.5 541,667 - -0.6 541,667 - +0.7 541,667 - -0.2 541,667 - +0.1 541,667 - +0.1 541,795 + A4249_RS02525 0.17 +0.3 541,795 + A4249_RS02525 0.17 +1.1 541,795 + A4249_RS02525 0.17 +0.3 541,795 + A4249_RS02525 0.17 +0.0 541,795 + A4249_RS02525 0.17 -2.3 541,796 - A4249_RS02525 0.17 +1.2 541,796 - A4249_RS02525 0.17 +0.2 541,796 - A4249_RS02525 0.17 +0.3 541,796 - A4249_RS02525 0.17 +1.1 541,796 - A4249_RS02525 0.17 +0.1 541,796 - A4249_RS02525 0.17 +0.3 541,940 - A4249_RS02525 0.34 +0.2 541,940 - A4249_RS02525 0.34 +0.7 541,940 - A4249_RS02525 0.34 -0.4 541,940 - A4249_RS02525 0.34 -0.9 541,990 + A4249_RS02525 0.40 -0.5 541,990 + A4249_RS02525 0.40 +0.3 541,990 + A4249_RS02525 0.40 -0.3 541,991 - A4249_RS02525 0.41 -0.2 541,991 - A4249_RS02525 0.41 +0.0 542,084 - A4249_RS02525 0.52 +0.4 542,084 - A4249_RS02525 0.52 -1.0 542,084 - A4249_RS02525 0.52 +0.1 542,164 + A4249_RS02525 0.62 -0.1 542,164 + A4249_RS02525 0.62 -0.3 542,164 + A4249_RS02525 0.62 +0.5 542,164 + A4249_RS02525 0.62 +0.3 542,164 + A4249_RS02525 0.62 -0.2 542,164 + A4249_RS02525 0.62 -0.4 542,164 + A4249_RS02525 0.62 +0.2 542,165 - A4249_RS02525 0.62 +0.1 542,165 - A4249_RS02525 0.62 -0.1 542,165 - A4249_RS02525 0.62 -0.6 542,165 - A4249_RS02525 0.62 -0.6 542,165 - A4249_RS02525 0.62 +0.3 542,486 + -0.1 542,486 + +0.2 542,486 + +0.1 542,486 + +1.1 542,487 - -0.2 542,487 - -0.0 542,487 - +0.2
Or see this region's nucleotide sequence