Experiment: Uracil 0.8921 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02660 and A4249_RS02665 are separated by 100 nucleotides A4249_RS02665 and A4249_RS02670 overlap by 4 nucleotides A4249_RS02670 and A4249_RS02675 are separated by 60 nucleotides
A4249_RS02660: A4249_RS02660 - hypothetical protein, at 568,782 to 569,771
_RS02660
A4249_RS02665: A4249_RS02665 - MBL fold metallo-hydrolase, at 569,872 to 570,957
_RS02665
A4249_RS02670: A4249_RS02670 - enoyl-CoA hydratase, at 570,954 to 571,727
_RS02670
A4249_RS02675: A4249_RS02675 - N-acetyltransferase, at 571,788 to 572,282
_RS02675
Position (kb) 569
570
571 Strain fitness (log2 ratio) -2
-1
0
1
2 at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.873 kb on + strand at 568.945 kb on + strand, within A4249_RS02660 at 568.945 kb on + strand, within A4249_RS02660 at 568.945 kb on + strand, within A4249_RS02660 at 568.945 kb on + strand, within A4249_RS02660 at 568.945 kb on + strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 568.946 kb on - strand, within A4249_RS02660 at 569.031 kb on + strand, within A4249_RS02660 at 569.248 kb on + strand, within A4249_RS02660 at 569.248 kb on + strand, within A4249_RS02660 at 569.248 kb on + strand, within A4249_RS02660 at 569.249 kb on - strand, within A4249_RS02660 at 569.249 kb on - strand, within A4249_RS02660 at 569.249 kb on - strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.298 kb on + strand, within A4249_RS02660 at 569.299 kb on - strand, within A4249_RS02660 at 569.299 kb on - strand, within A4249_RS02660 at 569.299 kb on - strand, within A4249_RS02660 at 569.299 kb on - strand, within A4249_RS02660 at 569.347 kb on + strand, within A4249_RS02660 at 569.348 kb on - strand, within A4249_RS02660 at 569.348 kb on - strand, within A4249_RS02660 at 569.424 kb on + strand, within A4249_RS02660 at 569.424 kb on + strand, within A4249_RS02660 at 569.424 kb on + strand, within A4249_RS02660 at 569.425 kb on - strand, within A4249_RS02660 at 569.425 kb on - strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.431 kb on + strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.432 kb on - strand, within A4249_RS02660 at 569.737 kb on - strand at 569.750 kb on + strand at 569.750 kb on + strand at 569.751 kb on - strand at 569.779 kb on + strand at 569.779 kb on + strand at 569.779 kb on + strand at 569.779 kb on + strand at 569.779 kb on + strand at 569.780 kb on - strand at 569.780 kb on - strand at 569.780 kb on - strand at 569.780 kb on - strand at 569.780 kb on - strand at 569.791 kb on + strand at 569.791 kb on + strand at 569.792 kb on - strand at 569.840 kb on + strand at 569.840 kb on + strand at 569.841 kb on - strand at 569.841 kb on - strand at 569.841 kb on - strand at 569.841 kb on - strand at 569.841 kb on - strand at 569.845 kb on - strand at 569.845 kb on - strand at 569.845 kb on - strand at 569.845 kb on - strand at 569.926 kb on + strand at 569.926 kb on + strand at 569.926 kb on + strand at 569.926 kb on + strand at 569.926 kb on + strand at 569.927 kb on - strand at 570.031 kb on + strand, within A4249_RS02665 at 570.031 kb on + strand, within A4249_RS02665 at 570.031 kb on + strand, within A4249_RS02665 at 570.031 kb on + strand, within A4249_RS02665 at 570.031 kb on + strand, within A4249_RS02665 at 570.032 kb on - strand, within A4249_RS02665 at 570.032 kb on - strand, within A4249_RS02665 at 570.032 kb on - strand, within A4249_RS02665 at 570.032 kb on - strand, within A4249_RS02665 at 570.077 kb on + strand, within A4249_RS02665 at 570.078 kb on - strand, within A4249_RS02665 at 570.078 kb on - strand, within A4249_RS02665 at 570.078 kb on - strand, within A4249_RS02665 at 570.078 kb on - strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.165 kb on + strand, within A4249_RS02665 at 570.166 kb on - strand, within A4249_RS02665 at 570.166 kb on - strand, within A4249_RS02665 at 570.166 kb on - strand, within A4249_RS02665 at 570.166 kb on - strand, within A4249_RS02665 at 570.166 kb on - strand, within A4249_RS02665 at 570.181 kb on - strand, within A4249_RS02665 at 570.200 kb on + strand, within A4249_RS02665 at 570.200 kb on + strand, within A4249_RS02665 at 570.201 kb on - strand, within A4249_RS02665 at 570.201 kb on - strand, within A4249_RS02665 at 570.201 kb on - strand, within A4249_RS02665 at 570.201 kb on - strand, within A4249_RS02665 at 570.201 kb on - strand, within A4249_RS02665 at 570.244 kb on + strand, within A4249_RS02665 at 570.244 kb on + strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.245 kb on - strand, within A4249_RS02665 at 570.307 kb on + strand, within A4249_RS02665 at 570.307 kb on + strand, within A4249_RS02665 at 570.307 kb on + strand, within A4249_RS02665 at 570.308 kb on - strand, within A4249_RS02665 at 570.308 kb on - strand, within A4249_RS02665 at 570.308 kb on - strand, within A4249_RS02665 at 570.308 kb on - strand, within A4249_RS02665 at 570.310 kb on + strand, within A4249_RS02665 at 570.310 kb on + strand, within A4249_RS02665 at 570.310 kb on + strand, within A4249_RS02665 at 570.310 kb on + strand, within A4249_RS02665 at 570.311 kb on - strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.403 kb on + strand, within A4249_RS02665 at 570.404 kb on - strand, within A4249_RS02665 at 570.404 kb on - strand, within A4249_RS02665 at 570.404 kb on - strand, within A4249_RS02665 at 570.404 kb on - strand, within A4249_RS02665 at 570.404 kb on - strand, within A4249_RS02665 at 570.477 kb on + strand, within A4249_RS02665 at 570.685 kb on + strand, within A4249_RS02665 at 570.686 kb on - strand, within A4249_RS02665 at 570.686 kb on - strand, within A4249_RS02665 at 570.686 kb on - strand, within A4249_RS02665 at 570.686 kb on - strand, within A4249_RS02665 at 570.686 kb on - strand, within A4249_RS02665 at 570.771 kb on + strand, within A4249_RS02665 at 570.772 kb on - strand, within A4249_RS02665 at 570.832 kb on - strand, within A4249_RS02665 at 570.865 kb on + strand at 570.865 kb on + strand at 570.865 kb on + strand at 570.965 kb on + strand at 570.965 kb on + strand at 570.965 kb on + strand at 570.965 kb on + strand at 570.965 kb on + strand at 570.966 kb on - strand at 570.966 kb on - strand at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.047 kb on + strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.048 kb on - strand, within A4249_RS02670 at 571.479 kb on - strand, within A4249_RS02670 at 571.725 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.773 kb on + strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.774 kb on - strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.807 kb on + strand at 571.808 kb on - strand at 571.819 kb on + strand at 571.957 kb on + strand, within A4249_RS02675
Per-strain Table
Position Strand Gene LocusTag Fraction Uracil 0.8921 mM (C) remove 568,873 + -0.3 568,873 + +1.1 568,873 + +0.1 568,873 + -0.7 568,873 + +0.0 568,873 + +1.0 568,873 + +0.0 568,873 + +0.7 568,945 + A4249_RS02660 0.16 -0.6 568,945 + A4249_RS02660 0.16 +0.2 568,945 + A4249_RS02660 0.16 +0.4 568,945 + A4249_RS02660 0.16 +0.3 568,945 + A4249_RS02660 0.16 +1.6 568,946 - A4249_RS02660 0.17 +0.4 568,946 - A4249_RS02660 0.17 -1.1 568,946 - A4249_RS02660 0.17 +0.6 568,946 - A4249_RS02660 0.17 -0.1 568,946 - A4249_RS02660 0.17 +0.6 568,946 - A4249_RS02660 0.17 +0.5 568,946 - A4249_RS02660 0.17 +0.0 568,946 - A4249_RS02660 0.17 -1.2 568,946 - A4249_RS02660 0.17 -0.2 568,946 - A4249_RS02660 0.17 +0.3 568,946 - A4249_RS02660 0.17 -0.6 568,946 - A4249_RS02660 0.17 +0.1 569,031 + A4249_RS02660 0.25 +1.8 569,248 + A4249_RS02660 0.47 -2.2 569,248 + A4249_RS02660 0.47 -0.0 569,248 + A4249_RS02660 0.47 +0.2 569,249 - A4249_RS02660 0.47 -0.0 569,249 - A4249_RS02660 0.47 +0.3 569,249 - A4249_RS02660 0.47 -0.0 569,298 + A4249_RS02660 0.52 -0.6 569,298 + A4249_RS02660 0.52 +1.4 569,298 + A4249_RS02660 0.52 +0.1 569,298 + A4249_RS02660 0.52 -1.0 569,298 + A4249_RS02660 0.52 -1.4 569,298 + A4249_RS02660 0.52 +1.8 569,299 - A4249_RS02660 0.52 +0.0 569,299 - A4249_RS02660 0.52 -0.3 569,299 - A4249_RS02660 0.52 +0.6 569,299 - A4249_RS02660 0.52 -0.5 569,347 + A4249_RS02660 0.57 +0.2 569,348 - A4249_RS02660 0.57 +0.4 569,348 - A4249_RS02660 0.57 -0.2 569,424 + A4249_RS02660 0.65 +0.1 569,424 + A4249_RS02660 0.65 -0.1 569,424 + A4249_RS02660 0.65 -0.2 569,425 - A4249_RS02660 0.65 +0.2 569,425 - A4249_RS02660 0.65 +0.8 569,431 + A4249_RS02660 0.66 -1.1 569,431 + A4249_RS02660 0.66 +0.6 569,431 + A4249_RS02660 0.66 +0.3 569,431 + A4249_RS02660 0.66 +0.3 569,431 + A4249_RS02660 0.66 -0.6 569,431 + A4249_RS02660 0.66 +0.1 569,432 - A4249_RS02660 0.66 -0.0 569,432 - A4249_RS02660 0.66 -0.4 569,432 - A4249_RS02660 0.66 -0.9 569,432 - A4249_RS02660 0.66 -0.8 569,432 - A4249_RS02660 0.66 -0.2 569,432 - A4249_RS02660 0.66 +1.2 569,432 - A4249_RS02660 0.66 -0.5 569,737 - -0.5 569,750 + +0.0 569,750 + +0.4 569,751 - -0.9 569,779 + -1.2 569,779 + -0.3 569,779 + -1.3 569,779 + -1.0 569,779 + +0.2 569,780 - +0.7 569,780 - -0.1 569,780 - +0.7 569,780 - +0.3 569,780 - -0.1 569,791 + -0.1 569,791 + +0.0 569,792 - -0.4 569,840 + -1.1 569,840 + -0.2 569,841 - -0.3 569,841 - -0.3 569,841 - +0.2 569,841 - -0.4 569,841 - +0.5 569,845 - +0.0 569,845 - -0.2 569,845 - -0.7 569,845 - -1.1 569,926 + +0.3 569,926 + -0.2 569,926 + +0.6 569,926 + -1.2 569,926 + -0.8 569,927 - +0.7 570,031 + A4249_RS02665 0.15 +0.2 570,031 + A4249_RS02665 0.15 +0.0 570,031 + A4249_RS02665 0.15 +0.3 570,031 + A4249_RS02665 0.15 +0.1 570,031 + A4249_RS02665 0.15 +0.2 570,032 - A4249_RS02665 0.15 +1.1 570,032 - A4249_RS02665 0.15 -2.0 570,032 - A4249_RS02665 0.15 +1.3 570,032 - A4249_RS02665 0.15 +0.5 570,077 + A4249_RS02665 0.19 +0.1 570,078 - A4249_RS02665 0.19 +0.3 570,078 - A4249_RS02665 0.19 +2.0 570,078 - A4249_RS02665 0.19 +0.3 570,078 - A4249_RS02665 0.19 +0.5 570,165 + A4249_RS02665 0.27 -0.2 570,165 + A4249_RS02665 0.27 +0.1 570,165 + A4249_RS02665 0.27 +0.0 570,165 + A4249_RS02665 0.27 -0.5 570,165 + A4249_RS02665 0.27 -0.9 570,165 + A4249_RS02665 0.27 +0.5 570,166 - A4249_RS02665 0.27 -1.2 570,166 - A4249_RS02665 0.27 +0.2 570,166 - A4249_RS02665 0.27 +0.4 570,166 - A4249_RS02665 0.27 +0.5 570,166 - A4249_RS02665 0.27 +0.3 570,181 - A4249_RS02665 0.28 -0.3 570,200 + A4249_RS02665 0.30 +0.6 570,200 + A4249_RS02665 0.30 +0.3 570,201 - A4249_RS02665 0.30 +0.7 570,201 - A4249_RS02665 0.30 +0.5 570,201 - A4249_RS02665 0.30 -2.4 570,201 - A4249_RS02665 0.30 +0.1 570,201 - A4249_RS02665 0.30 +0.2 570,244 + A4249_RS02665 0.34 -0.1 570,244 + A4249_RS02665 0.34 -1.2 570,245 - A4249_RS02665 0.34 +0.7 570,245 - A4249_RS02665 0.34 -0.8 570,245 - A4249_RS02665 0.34 -0.6 570,245 - A4249_RS02665 0.34 +0.4 570,245 - A4249_RS02665 0.34 +0.4 570,245 - A4249_RS02665 0.34 -0.6 570,307 + A4249_RS02665 0.40 +0.1 570,307 + A4249_RS02665 0.40 +0.5 570,307 + A4249_RS02665 0.40 +0.1 570,308 - A4249_RS02665 0.40 -0.2 570,308 - A4249_RS02665 0.40 -0.2 570,308 - A4249_RS02665 0.40 -1.5 570,308 - A4249_RS02665 0.40 +0.3 570,310 + A4249_RS02665 0.40 -0.3 570,310 + A4249_RS02665 0.40 +0.7 570,310 + A4249_RS02665 0.40 -0.1 570,310 + A4249_RS02665 0.40 -0.7 570,311 - A4249_RS02665 0.40 -0.6 570,403 + A4249_RS02665 0.49 +0.7 570,403 + A4249_RS02665 0.49 -0.9 570,403 + A4249_RS02665 0.49 +0.1 570,403 + A4249_RS02665 0.49 -0.3 570,403 + A4249_RS02665 0.49 -0.4 570,403 + A4249_RS02665 0.49 +1.3 570,404 - A4249_RS02665 0.49 -0.3 570,404 - A4249_RS02665 0.49 -0.1 570,404 - A4249_RS02665 0.49 -0.4 570,404 - A4249_RS02665 0.49 -0.1 570,404 - A4249_RS02665 0.49 +0.2 570,477 + A4249_RS02665 0.56 -0.4 570,685 + A4249_RS02665 0.75 +0.3 570,686 - A4249_RS02665 0.75 -0.1 570,686 - A4249_RS02665 0.75 +0.1 570,686 - A4249_RS02665 0.75 -0.6 570,686 - A4249_RS02665 0.75 -1.2 570,686 - A4249_RS02665 0.75 +0.3 570,771 + A4249_RS02665 0.83 +1.4 570,772 - A4249_RS02665 0.83 +0.4 570,832 - A4249_RS02665 0.88 -1.7 570,865 + +1.1 570,865 + +0.8 570,865 + +1.4 570,965 + -0.5 570,965 + -0.7 570,965 + -0.1 570,965 + +1.0 570,965 + -0.7 570,966 - -0.6 570,966 - +0.6 571,047 + A4249_RS02670 0.12 -0.1 571,047 + A4249_RS02670 0.12 -0.1 571,047 + A4249_RS02670 0.12 +0.2 571,047 + A4249_RS02670 0.12 -0.2 571,047 + A4249_RS02670 0.12 +1.1 571,047 + A4249_RS02670 0.12 -0.2 571,047 + A4249_RS02670 0.12 -0.0 571,047 + A4249_RS02670 0.12 -0.1 571,047 + A4249_RS02670 0.12 -0.2 571,047 + A4249_RS02670 0.12 +0.3 571,047 + A4249_RS02670 0.12 +0.4 571,047 + A4249_RS02670 0.12 -0.0 571,047 + A4249_RS02670 0.12 -0.4 571,047 + A4249_RS02670 0.12 -0.0 571,048 - A4249_RS02670 0.12 +0.1 571,048 - A4249_RS02670 0.12 -0.0 571,048 - A4249_RS02670 0.12 +0.1 571,048 - A4249_RS02670 0.12 +0.2 571,048 - A4249_RS02670 0.12 -0.4 571,048 - A4249_RS02670 0.12 -0.1 571,048 - A4249_RS02670 0.12 -0.3 571,048 - A4249_RS02670 0.12 -0.0 571,479 - A4249_RS02670 0.68 -0.3 571,725 + -0.6 571,773 + +0.6 571,773 + +0.5 571,773 + -0.2 571,773 + -0.0 571,774 - -0.1 571,774 - -0.1 571,774 - +0.9 571,774 - -0.2 571,774 - +0.0 571,807 + +0.0 571,807 + -0.4 571,807 + +0.4 571,807 + -0.9 571,807 + +0.6 571,808 - +1.1 571,819 + +0.2 571,957 + A4249_RS02675 0.34 -0.2
Or see this region's nucleotide sequence