Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaF and wcaE are separated by 15 nucleotides wcaE and wcaD are separated by 10 nucleotides wcaD and wcaC overlap by 26 nucleotides
MCAODC_20600: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 931,605 to 932,153
wcaF
MCAODC_20605: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 932,169 to 932,915
wcaE
MCAODC_20610: wcaD - putative colanic acid polymerase WcaD, at 932,926 to 934,143
wcaD
MCAODC_20615: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 934,118 to 935,335
wcaC
Position (kb) 932
933
934
935 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 931.933 kb on - strand, within wcaF at 932.041 kb on - strand, within wcaF at 932.097 kb on - strand, within wcaF at 932.100 kb on - strand at 932.207 kb on + strand at 932.208 kb on - strand at 932.615 kb on - strand, within wcaE at 932.705 kb on - strand, within wcaE at 932.737 kb on - strand, within wcaE at 932.744 kb on - strand, within wcaE at 932.922 kb on - strand at 932.959 kb on - strand at 933.535 kb on - strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.799 kb on + strand, within wcaD at 933.855 kb on + strand, within wcaD at 933.873 kb on + strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.875 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD at 933.936 kb on - strand, within wcaD at 933.940 kb on - strand, within wcaD at 934.016 kb on - strand, within wcaD at 934.094 kb on + strand at 934.095 kb on - strand at 934.115 kb on + strand at 934.115 kb on + strand at 934.143 kb on + strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.501 kb on + strand, within wcaC at 934.501 kb on + strand, within wcaC at 934.501 kb on + strand, within wcaC at 934.511 kb on - strand, within wcaC at 934.565 kb on - strand, within wcaC at 934.565 kb on - strand, within wcaC at 934.596 kb on - strand, within wcaC at 934.670 kb on - strand, within wcaC at 934.675 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.847 kb on - strand, within wcaC at 934.847 kb on - strand, within wcaC at 934.847 kb on - strand, within wcaC at 935.006 kb on - strand, within wcaC at 935.069 kb on - strand, within wcaC at 935.082 kb on - strand, within wcaC
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 931,933 - wcaF MCAODC_20600 0.60 +0.7 932,041 - wcaF MCAODC_20600 0.79 -1.6 932,097 - wcaF MCAODC_20600 0.90 -2.8 932,100 - -3.2 932,207 + -0.7 932,208 - -0.3 932,615 - wcaE MCAODC_20605 0.60 -0.7 932,705 - wcaE MCAODC_20605 0.72 +0.0 932,737 - wcaE MCAODC_20605 0.76 +2.2 932,744 - wcaE MCAODC_20605 0.77 -2.7 932,922 - -0.3 932,959 - -0.5 933,535 - wcaD MCAODC_20610 0.50 -1.1 933,671 + wcaD MCAODC_20610 0.61 -0.5 933,671 + wcaD MCAODC_20610 0.61 +0.8 933,799 + wcaD MCAODC_20610 0.72 +1.2 933,855 + wcaD MCAODC_20610 0.76 +1.2 933,873 + wcaD MCAODC_20610 0.78 +0.5 933,874 - wcaD MCAODC_20610 0.78 -1.8 933,874 - wcaD MCAODC_20610 0.78 -0.9 933,875 + wcaD MCAODC_20610 0.78 +0.8 933,888 + wcaD MCAODC_20610 0.79 +0.7 933,888 + wcaD MCAODC_20610 0.79 +0.6 933,888 + wcaD MCAODC_20610 0.79 +1.4 933,902 + wcaD MCAODC_20610 0.80 +0.3 933,902 + wcaD MCAODC_20610 0.80 -1.0 933,936 - wcaD MCAODC_20610 0.83 +1.2 933,940 - wcaD MCAODC_20610 0.83 -0.7 934,016 - wcaD MCAODC_20610 0.89 +1.3 934,094 + +0.7 934,095 - -0.5 934,115 + -0.9 934,115 + -1.8 934,143 + -2.7 934,144 - +0.2 934,144 - -0.5 934,144 - -2.3 934,144 - -2.0 934,501 + wcaC MCAODC_20615 0.31 +0.3 934,501 + wcaC MCAODC_20615 0.31 -0.6 934,501 + wcaC MCAODC_20615 0.31 -0.1 934,511 - wcaC MCAODC_20615 0.32 +0.3 934,565 - wcaC MCAODC_20615 0.37 +1.0 934,565 - wcaC MCAODC_20615 0.37 -1.7 934,596 - wcaC MCAODC_20615 0.39 -0.6 934,670 - wcaC MCAODC_20615 0.45 +1.1 934,675 + wcaC MCAODC_20615 0.46 -0.4 934,846 + wcaC MCAODC_20615 0.60 -0.8 934,846 + wcaC MCAODC_20615 0.60 +0.6 934,846 + wcaC MCAODC_20615 0.60 +1.4 934,847 - wcaC MCAODC_20615 0.60 +0.7 934,847 - wcaC MCAODC_20615 0.60 -2.2 934,847 - wcaC MCAODC_20615 0.60 -0.0 935,006 - wcaC MCAODC_20615 0.73 +2.4 935,069 - wcaC MCAODC_20615 0.78 -0.1 935,082 - wcaC MCAODC_20615 0.79 -0.4
Or see this region's nucleotide sequence