Strain Fitness in Escherichia coli ECRC101 around MCAODC_20525

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzx and wcaA overlap by 14 nucleotideswcaA and wzy overlap by 32 nucleotideswzy and wcaA overlap by 4 nucleotides MCAODC_20515: wzx - O157 family O-antigen flippase, at 911,108 to 912,499 wzx MCAODC_20520: wcaA - glycosyl transferase, at 912,486 to 913,232 wcaA MCAODC_20525: wzy - O157 family O-antigen polymerase, at 913,201 to 914,385 wzy MCAODC_20530: wcaA - glycosyl transferase, at 914,382 to 915,164 wcaA Position (kb) 913 914 915Strain fitness (log2 ratio) -3 -2 -1 0 1at 913.500 kb on - strand, within wzyat 913.677 kb on - strand, within wzyat 913.699 kb on + strand, within wzyat 913.735 kb on - strand, within wzyat 913.820 kb on - strand, within wzyat 914.095 kb on - strand, within wzyat 914.137 kb on + strand, within wzyat 914.462 kb on - strand, within wcaAat 914.504 kb on - strand, within wcaAat 914.717 kb on - strand, within wcaAat 915.111 kb on - strandat 915.301 kb on - strandat 915.309 kb on - strandat 915.377 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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913,500 - wzy MCAODC_20525 0.25 -1.6
913,677 - wzy MCAODC_20525 0.40 -1.9
913,699 + wzy MCAODC_20525 0.42 -1.9
913,735 - wzy MCAODC_20525 0.45 -2.2
913,820 - wzy MCAODC_20525 0.52 -2.8
914,095 - wzy MCAODC_20525 0.75 -3.6
914,137 + wzy MCAODC_20525 0.79 -0.7
914,462 - wcaA MCAODC_20530 0.10 -2.9
914,504 - wcaA MCAODC_20530 0.16 -0.2
914,717 - wcaA MCAODC_20530 0.43 -3.6
915,111 - -1.4
915,301 - +0.6
915,309 - -2.2
915,377 - -0.3

Or see this region's nucleotide sequence