Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecA and xerD are separated by 356 nucleotides xerD and MCAODC_19385 are separated by 5 nucleotides MCAODC_19385 and MCAODC_19390 are separated by 29 nucleotides
MCAODC_19375: yecA - UPF0149 family protein YecA, at 721,373 to 722,038
yecA
MCAODC_19380: xerD - integrase, at 722,395 to 723,396
xerD
MCAODC_19385: MCAODC_19385 - DUF2511 domain-containing protein, at 723,402 to 723,749
_19385
MCAODC_19390: MCAODC_19390 - Putative transmembrane protein, at 723,779 to 724,429
_19390
Position (kb) 722
723
724 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 721.536 kb on - strand, within yecA at 721.595 kb on - strand, within yecA at 721.678 kb on - strand, within yecA at 721.855 kb on - strand, within yecA at 721.932 kb on - strand, within yecA at 722.074 kb on + strand at 722.074 kb on + strand at 722.075 kb on - strand at 722.079 kb on + strand at 722.079 kb on + strand at 722.080 kb on - strand at 722.080 kb on - strand at 722.080 kb on - strand at 722.103 kb on - strand at 722.352 kb on + strand at 722.371 kb on - strand at 722.371 kb on - strand at 722.438 kb on - strand at 722.470 kb on - strand at 722.470 kb on - strand at 722.488 kb on - strand at 722.561 kb on - strand, within xerD at 722.573 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.631 kb on - strand, within xerD at 722.879 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.990 kb on - strand, within xerD at 722.994 kb on - strand, within xerD at 723.081 kb on + strand, within xerD at 723.188 kb on + strand, within xerD at 723.188 kb on + strand, within xerD at 723.189 kb on - strand, within xerD at 723.189 kb on - strand, within xerD at 723.261 kb on + strand, within xerD at 723.284 kb on + strand, within xerD at 723.285 kb on - strand, within xerD at 723.370 kb on - strand at 723.407 kb on + strand at 723.407 kb on + strand at 723.476 kb on + strand, within MCAODC_19385 at 723.477 kb on - strand, within MCAODC_19385 at 723.501 kb on - strand, within MCAODC_19385 at 723.505 kb on - strand, within MCAODC_19385 at 723.562 kb on - strand, within MCAODC_19385 at 723.562 kb on - strand, within MCAODC_19385 at 723.570 kb on - strand, within MCAODC_19385 at 723.675 kb on - strand, within MCAODC_19385 at 723.869 kb on + strand, within MCAODC_19390 at 724.062 kb on - strand, within MCAODC_19390 at 724.074 kb on - strand, within MCAODC_19390 at 724.103 kb on - strand, within MCAODC_19390 at 724.145 kb on - strand, within MCAODC_19390 at 724.163 kb on - strand, within MCAODC_19390 at 724.211 kb on - strand, within MCAODC_19390 at 724.243 kb on + strand, within MCAODC_19390 at 724.337 kb on - strand, within MCAODC_19390 at 724.351 kb on - strand, within MCAODC_19390 at 724.351 kb on - strand, within MCAODC_19390
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 721,536 - yecA MCAODC_19375 0.24 +0.3 721,595 - yecA MCAODC_19375 0.33 +0.9 721,678 - yecA MCAODC_19375 0.46 -0.5 721,855 - yecA MCAODC_19375 0.72 -0.5 721,932 - yecA MCAODC_19375 0.84 +2.1 722,074 + +0.1 722,074 + +0.2 722,075 - +1.9 722,079 + -0.2 722,079 + -1.3 722,080 - +0.1 722,080 - +0.2 722,080 - -2.9 722,103 - +0.9 722,352 + -1.2 722,371 - -2.0 722,371 - -0.3 722,438 - -1.6 722,470 - -2.8 722,470 - -0.2 722,488 - -0.3 722,561 - xerD MCAODC_19380 0.17 -0.3 722,573 - xerD MCAODC_19380 0.18 -3.5 722,631 - xerD MCAODC_19380 0.24 -1.0 722,631 - xerD MCAODC_19380 0.24 +0.6 722,631 - xerD MCAODC_19380 0.24 -0.2 722,631 - xerD MCAODC_19380 0.24 -0.6 722,879 - xerD MCAODC_19380 0.48 +0.7 722,990 - xerD MCAODC_19380 0.59 +0.5 722,990 - xerD MCAODC_19380 0.59 +0.3 722,990 - xerD MCAODC_19380 0.59 +1.0 722,994 - xerD MCAODC_19380 0.60 +0.6 723,081 + xerD MCAODC_19380 0.68 +1.5 723,188 + xerD MCAODC_19380 0.79 +0.2 723,188 + xerD MCAODC_19380 0.79 -1.1 723,189 - xerD MCAODC_19380 0.79 +0.4 723,189 - xerD MCAODC_19380 0.79 +0.4 723,261 + xerD MCAODC_19380 0.86 +0.4 723,284 + xerD MCAODC_19380 0.89 -0.1 723,285 - xerD MCAODC_19380 0.89 -1.6 723,370 - -0.6 723,407 + +0.3 723,407 + -0.6 723,476 + MCAODC_19385 0.21 -0.4 723,477 - MCAODC_19385 0.22 -0.6 723,501 - MCAODC_19385 0.28 +0.1 723,505 - MCAODC_19385 0.30 +1.7 723,562 - MCAODC_19385 0.46 +0.7 723,562 - MCAODC_19385 0.46 -1.8 723,570 - MCAODC_19385 0.48 +0.7 723,675 - MCAODC_19385 0.78 +0.2 723,869 + MCAODC_19390 0.14 -0.5 724,062 - MCAODC_19390 0.43 -0.2 724,074 - MCAODC_19390 0.45 +0.1 724,103 - MCAODC_19390 0.50 -0.2 724,145 - MCAODC_19390 0.56 +0.2 724,163 - MCAODC_19390 0.59 -0.6 724,211 - MCAODC_19390 0.66 -2.8 724,243 + MCAODC_19390 0.71 -1.2 724,337 - MCAODC_19390 0.86 -0.5 724,351 - MCAODC_19390 0.88 -0.3 724,351 - MCAODC_19390 0.88 +1.2
Or see this region's nucleotide sequence