Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxG and rsxE are separated by 3 nucleotides rsxE and nth overlap by 1 nucleotides nth and MCAODC_17890 are separated by 127 nucleotides MCAODC_17890 and dtpA are separated by 282 nucleotides
MCAODC_17875: rsxG - electron transport complex subunit RsxG, at 439,200 to 439,820
rsxG
MCAODC_17880: rsxE - electron transport complex subunit RsxE, at 439,824 to 440,519
rsxE
MCAODC_17885: nth - endonuclease III, at 440,519 to 441,154
nth
MCAODC_17890: MCAODC_17890 - hypothetical protein, at 441,282 to 441,482
_17890
MCAODC_17895: dtpA - dipeptide/tripeptide permease DtpA, at 441,765 to 443,267
dtpA
Position (kb) 440
441
442 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 439.743 kb on - strand, within rsxG at 439.818 kb on + strand at 439.959 kb on - strand, within rsxE at 440.037 kb on + strand, within rsxE at 440.317 kb on + strand, within rsxE at 440.317 kb on + strand, within rsxE at 440.317 kb on + strand, within rsxE at 440.335 kb on + strand, within rsxE at 440.625 kb on + strand, within nth at 440.659 kb on + strand, within nth at 440.765 kb on - strand, within nth at 440.782 kb on + strand, within nth at 440.895 kb on + strand, within nth at 440.953 kb on + strand, within nth at 441.073 kb on + strand, within nth at 441.110 kb on - strand at 441.131 kb on + strand at 441.198 kb on - strand at 441.259 kb on - strand at 441.663 kb on - strand at 441.684 kb on + strand at 441.684 kb on + strand at 441.714 kb on + strand at 441.834 kb on + strand at 441.835 kb on - strand at 441.893 kb on + strand at 442.003 kb on + strand, within dtpA at 442.025 kb on + strand, within dtpA at 442.037 kb on + strand, within dtpA at 442.069 kb on - strand, within dtpA at 442.110 kb on + strand, within dtpA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 439,743 - rsxG MCAODC_17875 0.87 +2.7 439,818 + +1.7 439,959 - rsxE MCAODC_17880 0.19 +0.1 440,037 + rsxE MCAODC_17880 0.31 -0.8 440,317 + rsxE MCAODC_17880 0.71 -2.5 440,317 + rsxE MCAODC_17880 0.71 -0.0 440,317 + rsxE MCAODC_17880 0.71 -1.7 440,335 + rsxE MCAODC_17880 0.73 -1.1 440,625 + nth MCAODC_17885 0.17 -0.8 440,659 + nth MCAODC_17885 0.22 -1.3 440,765 - nth MCAODC_17885 0.39 +0.1 440,782 + nth MCAODC_17885 0.41 -1.4 440,895 + nth MCAODC_17885 0.59 +1.0 440,953 + nth MCAODC_17885 0.68 -0.7 441,073 + nth MCAODC_17885 0.87 -0.5 441,110 - -1.6 441,131 + +0.2 441,198 - +0.3 441,259 - +0.0 441,663 - +0.0 441,684 + +0.0 441,684 + +3.1 441,714 + +1.2 441,834 + -1.2 441,835 - -2.2 441,893 + -2.1 442,003 + dtpA MCAODC_17895 0.16 +0.7 442,025 + dtpA MCAODC_17895 0.17 +0.6 442,037 + dtpA MCAODC_17895 0.18 +1.0 442,069 - dtpA MCAODC_17895 0.20 +0.8 442,110 + dtpA MCAODC_17895 0.23 -1.0
Or see this region's nucleotide sequence