Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yneG and glsB overlap by 1 nucleotides glsB and sad are separated by 63 nucleotides sad and yneJ are separated by 100 nucleotides
MCAODC_16805: yneG - Uncharacterized protein YneG, at 273,950 to 274,309
yneG
MCAODC_16810: glsB - glutaminase B, at 274,309 to 275,235
glsB
MCAODC_16815: sad - succinate-semialdehyde dehydrogenase, at 275,299 to 276,687
sad
MCAODC_16820: yneJ - Uncharacterized HTH-type transcriptional regulator YneJ, at 276,788 to 277,669
yneJ
Position (kb)
275
276
277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 274.510 kb on - strand, within glsB at 274.576 kb on - strand, within glsB at 274.786 kb on - strand, within glsB at 274.861 kb on - strand, within glsB at 274.934 kb on - strand, within glsB at 275.097 kb on + strand, within glsB at 275.103 kb on + strand, within glsB at 275.104 kb on - strand, within glsB at 275.164 kb on - strand at 275.276 kb on - strand at 275.291 kb on - strand at 275.651 kb on + strand, within sad at 275.866 kb on - strand, within sad at 275.965 kb on - strand, within sad at 276.132 kb on - strand, within sad at 276.133 kb on + strand, within sad at 276.134 kb on - strand, within sad at 276.134 kb on - strand, within sad at 276.325 kb on - strand, within sad at 276.352 kb on - strand, within sad at 276.539 kb on - strand, within sad at 276.539 kb on - strand, within sad at 276.539 kb on - strand, within sad at 276.540 kb on + strand, within sad at 276.858 kb on + strand at 276.921 kb on + strand, within yneJ at 276.957 kb on + strand, within yneJ at 276.974 kb on - strand, within yneJ at 277.175 kb on - strand, within yneJ at 277.217 kb on - strand, within yneJ at 277.238 kb on - strand, within yneJ at 277.238 kb on - strand, within yneJ at 277.336 kb on + strand, within yneJ at 277.498 kb on + strand, within yneJ at 277.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 274,510 - glsB MCAODC_16810 0.22 +0.1 274,576 - glsB MCAODC_16810 0.29 -1.6 274,786 - glsB MCAODC_16810 0.51 -1.3 274,861 - glsB MCAODC_16810 0.60 -2.3 274,934 - glsB MCAODC_16810 0.67 -2.5 275,097 + glsB MCAODC_16810 0.85 +1.0 275,103 + glsB MCAODC_16810 0.86 -2.8 275,104 - glsB MCAODC_16810 0.86 -1.9 275,164 - +0.6 275,276 - +0.9 275,291 - -0.2 275,651 + sad MCAODC_16815 0.25 +0.7 275,866 - sad MCAODC_16815 0.41 +1.3 275,965 - sad MCAODC_16815 0.48 +0.2 276,132 - sad MCAODC_16815 0.60 +1.5 276,133 + sad MCAODC_16815 0.60 -0.2 276,134 - sad MCAODC_16815 0.60 -0.1 276,134 - sad MCAODC_16815 0.60 -0.1 276,325 - sad MCAODC_16815 0.74 -0.2 276,352 - sad MCAODC_16815 0.76 -1.0 276,539 - sad MCAODC_16815 0.89 +1.0 276,539 - sad MCAODC_16815 0.89 +0.5 276,539 - sad MCAODC_16815 0.89 -1.1 276,540 + sad MCAODC_16815 0.89 -0.8 276,858 + -1.1 276,921 + yneJ MCAODC_16820 0.15 +2.3 276,957 + yneJ MCAODC_16820 0.19 +0.4 276,974 - yneJ MCAODC_16820 0.21 +1.1 277,175 - yneJ MCAODC_16820 0.44 +0.8 277,217 - yneJ MCAODC_16820 0.49 +0.0 277,238 - yneJ MCAODC_16820 0.51 +1.0 277,238 - yneJ MCAODC_16820 0.51 +0.3 277,336 + yneJ MCAODC_16820 0.62 +0.2 277,498 + yneJ MCAODC_16820 0.80 -0.9 277,622 - -0.8
Or see this region's nucleotide sequence