Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt repE and sopA are separated by 587 nucleotides sopA and sopA overlap by 1 nucleotides
MCAODC_15425: repE - replication initiation protein RepE, at 69,455 to 70,210
repE
MCAODC_15430: sopA - plasmid-partitioning protein SopA, at 70,798 to 71,964
sopA
MCAODC_15435: sopA - plasmid-partitioning protein SopA, at 71,964 to 72,935
sopA
Position (kb) 70
71
72 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 69.833 kb on + strand, within repE at 69.840 kb on + strand, within repE at 69.840 kb on + strand, within repE at 69.841 kb on - strand, within repE at 69.841 kb on - strand, within repE at 69.841 kb on - strand, within repE at 69.841 kb on - strand, within repE at 69.841 kb on - strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.844 kb on + strand, within repE at 69.845 kb on - strand, within repE at 69.845 kb on - strand, within repE at 69.845 kb on - strand, within repE at 69.845 kb on - strand, within repE at 69.845 kb on - strand, within repE at 69.935 kb on + strand, within repE at 69.935 kb on + strand, within repE at 69.935 kb on + strand, within repE at 69.950 kb on + strand, within repE at 69.951 kb on - strand, within repE at 69.973 kb on + strand, within repE at 69.973 kb on + strand, within repE at 70.020 kb on + strand, within repE at 70.020 kb on + strand, within repE at 70.054 kb on + strand, within repE at 70.054 kb on + strand, within repE at 70.054 kb on + strand, within repE at 70.055 kb on - strand, within repE at 70.055 kb on - strand, within repE at 70.055 kb on - strand, within repE at 70.130 kb on + strand, within repE at 70.130 kb on + strand, within repE at 70.131 kb on - strand, within repE at 70.131 kb on - strand, within repE at 70.131 kb on - strand, within repE at 70.131 kb on - strand, within repE at 70.166 kb on - strand at 70.166 kb on - strand at 70.221 kb on - strand at 70.265 kb on + strand at 70.265 kb on + strand at 70.266 kb on - strand at 70.275 kb on - strand at 70.425 kb on + strand at 70.453 kb on + strand at 70.463 kb on + strand at 70.463 kb on + strand at 70.464 kb on - strand at 70.464 kb on - strand at 70.466 kb on + strand at 70.466 kb on + strand at 70.492 kb on + strand at 70.492 kb on + strand at 70.493 kb on - strand at 70.493 kb on - strand at 70.499 kb on - strand at 70.499 kb on - strand at 70.524 kb on - strand at 70.544 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.552 kb on - strand at 70.552 kb on - strand at 70.552 kb on - strand at 70.552 kb on - strand at 70.597 kb on + strand at 70.633 kb on + strand at 70.636 kb on + strand at 70.636 kb on + strand at 70.637 kb on - strand at 70.637 kb on - strand at 70.649 kb on + strand at 70.649 kb on + strand at 70.650 kb on - strand at 70.662 kb on + strand at 70.675 kb on - strand at 70.686 kb on + strand at 70.783 kb on + strand at 70.829 kb on + strand at 70.922 kb on + strand, within sopA at 71.043 kb on + strand, within sopA at 71.058 kb on + strand, within sopA at 71.064 kb on + strand, within sopA at 71.065 kb on - strand, within sopA at 71.204 kb on + strand, within sopA at 71.204 kb on + strand, within sopA at 71.280 kb on + strand, within sopA at 71.338 kb on + strand, within sopA at 71.439 kb on + strand, within sopA at 71.550 kb on + strand, within sopA at 71.691 kb on + strand, within sopA at 71.715 kb on + strand, within sopA at 71.715 kb on + strand, within sopA at 71.718 kb on + strand, within sopA at 71.718 kb on + strand, within sopA at 71.718 kb on + strand, within sopA at 71.792 kb on + strand, within sopA at 71.814 kb on + strand, within sopA at 71.962 kb on + strand at 71.963 kb on - strand at 71.963 kb on - strand at 71.993 kb on + strand at 71.994 kb on - strand at 72.112 kb on + strand, within sopA at 72.610 kb on + strand, within sopA at 72.611 kb on - strand, within sopA at 72.664 kb on + strand, within sopA at 72.758 kb on + strand, within sopA at 72.782 kb on + strand, within sopA at 72.828 kb on + strand, within sopA at 72.828 kb on + strand, within sopA at 72.829 kb on - strand, within sopA at 72.829 kb on - strand, within sopA at 72.949 kb on + strand at 72.950 kb on - strand at 72.954 kb on + strand at 72.955 kb on - strand at 72.955 kb on - strand at 72.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 69,833 + repE MCAODC_15425 0.50 -3.8 69,840 + repE MCAODC_15425 0.51 +0.4 69,840 + repE MCAODC_15425 0.51 +1.1 69,841 - repE MCAODC_15425 0.51 -1.5 69,841 - repE MCAODC_15425 0.51 -0.9 69,841 - repE MCAODC_15425 0.51 -0.2 69,841 - repE MCAODC_15425 0.51 -0.9 69,841 - repE MCAODC_15425 0.51 +0.4 69,844 + repE MCAODC_15425 0.51 -1.0 69,844 + repE MCAODC_15425 0.51 -0.2 69,844 + repE MCAODC_15425 0.51 +0.9 69,844 + repE MCAODC_15425 0.51 +1.3 69,844 + repE MCAODC_15425 0.51 -0.1 69,844 + repE MCAODC_15425 0.51 +2.3 69,844 + repE MCAODC_15425 0.51 -0.3 69,844 + repE MCAODC_15425 0.51 -0.6 69,845 - repE MCAODC_15425 0.52 -0.9 69,845 - repE MCAODC_15425 0.52 +1.7 69,845 - repE MCAODC_15425 0.52 -0.6 69,845 - repE MCAODC_15425 0.52 +0.2 69,845 - repE MCAODC_15425 0.52 +1.1 69,935 + repE MCAODC_15425 0.63 +0.4 69,935 + repE MCAODC_15425 0.63 +2.0 69,935 + repE MCAODC_15425 0.63 +0.3 69,950 + repE MCAODC_15425 0.65 +0.2 69,951 - repE MCAODC_15425 0.66 +0.3 69,973 + repE MCAODC_15425 0.69 -0.5 69,973 + repE MCAODC_15425 0.69 +0.7 70,020 + repE MCAODC_15425 0.75 -2.3 70,020 + repE MCAODC_15425 0.75 +1.2 70,054 + repE MCAODC_15425 0.79 -0.1 70,054 + repE MCAODC_15425 0.79 -0.8 70,054 + repE MCAODC_15425 0.79 -3.1 70,055 - repE MCAODC_15425 0.79 +0.7 70,055 - repE MCAODC_15425 0.79 +2.1 70,055 - repE MCAODC_15425 0.79 +0.6 70,130 + repE MCAODC_15425 0.89 +0.3 70,130 + repE MCAODC_15425 0.89 -0.2 70,131 - repE MCAODC_15425 0.89 -0.0 70,131 - repE MCAODC_15425 0.89 -0.6 70,131 - repE MCAODC_15425 0.89 -0.5 70,131 - repE MCAODC_15425 0.89 -0.1 70,166 - +0.5 70,166 - +0.8 70,221 - +0.9 70,265 + +0.3 70,265 + -0.8 70,266 - -1.1 70,275 - +1.3 70,425 + -0.8 70,453 + +2.1 70,463 + -0.3 70,463 + -1.3 70,464 - -3.0 70,464 - +0.4 70,466 + -3.5 70,466 + -0.0 70,492 + +1.8 70,492 + -1.2 70,493 - +1.3 70,493 - +0.6 70,499 - +0.8 70,499 - +0.1 70,524 - +0.1 70,544 + -1.2 70,551 + -1.4 70,551 + -0.2 70,551 + -0.7 70,551 + -1.2 70,552 - -1.3 70,552 - -0.4 70,552 - -0.5 70,552 - -0.4 70,597 + +0.8 70,633 + +0.1 70,636 + -1.6 70,636 + -0.4 70,637 - -0.5 70,637 - +0.0 70,649 + -2.6 70,649 + -1.0 70,650 - +0.2 70,662 + +0.1 70,675 - -2.6 70,686 + -1.6 70,783 + -0.9 70,829 + -0.1 70,922 + sopA MCAODC_15430 0.11 -1.1 71,043 + sopA MCAODC_15430 0.21 +0.1 71,058 + sopA MCAODC_15430 0.22 -2.3 71,064 + sopA MCAODC_15430 0.23 -0.7 71,065 - sopA MCAODC_15430 0.23 +2.3 71,204 + sopA MCAODC_15430 0.35 -0.8 71,204 + sopA MCAODC_15430 0.35 +1.1 71,280 + sopA MCAODC_15430 0.41 -1.1 71,338 + sopA MCAODC_15430 0.46 -1.6 71,439 + sopA MCAODC_15430 0.55 +5.1 71,550 + sopA MCAODC_15430 0.64 -0.2 71,691 + sopA MCAODC_15430 0.77 -1.4 71,715 + sopA MCAODC_15430 0.79 +1.1 71,715 + sopA MCAODC_15430 0.79 -0.2 71,718 + sopA MCAODC_15430 0.79 -0.6 71,718 + sopA MCAODC_15430 0.79 +0.8 71,718 + sopA MCAODC_15430 0.79 +0.5 71,792 + sopA MCAODC_15430 0.85 +2.1 71,814 + sopA MCAODC_15430 0.87 -2.1 71,962 + -1.9 71,963 - -1.0 71,963 - -0.4 71,993 + +0.7 71,994 - +0.1 72,112 + sopA MCAODC_15435 0.15 -0.7 72,610 + sopA MCAODC_15435 0.66 -0.9 72,611 - sopA MCAODC_15435 0.67 +0.5 72,664 + sopA MCAODC_15435 0.72 +0.3 72,758 + sopA MCAODC_15435 0.82 +0.5 72,782 + sopA MCAODC_15435 0.84 +0.3 72,828 + sopA MCAODC_15435 0.89 +0.8 72,828 + sopA MCAODC_15435 0.89 -1.5 72,829 - sopA MCAODC_15435 0.89 +0.5 72,829 - sopA MCAODC_15435 0.89 +1.1 72,949 + +0.2 72,950 - +0.5 72,954 + -0.2 72,955 - -1.1 72,955 - -3.5 72,955 - -0.7
Or see this region's nucleotide sequence