Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfeR and nfeF are separated by 287 nucleotides nfeF and Ile2_trna are separated by 53 nucleotides Ile2_trna and mug are separated by 124 nucleotides mug and rpoD are separated by 78 nucleotides
MCAODC_09150: nfeR - DNA-binding transcriptional regulator NfeR, at 1,827,833 to 1,828,456
nfeR
MCAODC_09155: nfeF - NADPH-dependent ferric chelate reductase, at 1,828,744 to 1,829,508
nfeF
MCAODC_09160: Ile2_trna - tRNA-Ile2(cat), at 1,829,562 to 1,829,637
_trna
MCAODC_09165: mug - G/U mismatch-specific DNA glycosylase, at 1,829,762 to 1,830,268
mug
MCAODC_09170: rpoD - RNA polymerase sigma factor RpoD, at 1,830,347 to 1,832,188
rpoD
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1827.746 kb on + strand at 1827.757 kb on + strand at 1827.766 kb on + strand at 1827.767 kb on - strand at 1827.835 kb on - strand at 1827.851 kb on - strand at 1827.908 kb on - strand, within nfeR at 1827.908 kb on - strand, within nfeR at 1827.927 kb on - strand, within nfeR at 1828.094 kb on - strand, within nfeR at 1828.155 kb on + strand, within nfeR at 1828.180 kb on - strand, within nfeR at 1828.430 kb on - strand at 1828.430 kb on - strand at 1828.558 kb on + strand at 1828.558 kb on + strand at 1828.597 kb on + strand at 1828.597 kb on + strand at 1828.597 kb on + strand at 1828.597 kb on + strand at 1828.597 kb on + strand at 1828.597 kb on + strand at 1828.598 kb on - strand at 1828.637 kb on + strand at 1828.638 kb on - strand at 1828.643 kb on + strand at 1828.643 kb on + strand at 1828.644 kb on - strand at 1828.644 kb on - strand at 1828.670 kb on - strand at 1828.714 kb on + strand at 1828.825 kb on - strand, within nfeF at 1828.825 kb on - strand, within nfeF at 1828.878 kb on + strand, within nfeF at 1828.879 kb on - strand, within nfeF at 1829.006 kb on - strand, within nfeF at 1829.006 kb on - strand, within nfeF at 1829.007 kb on + strand, within nfeF at 1829.008 kb on - strand, within nfeF at 1829.027 kb on + strand, within nfeF at 1829.028 kb on - strand, within nfeF at 1829.028 kb on - strand, within nfeF at 1829.056 kb on - strand, within nfeF at 1829.165 kb on - strand, within nfeF at 1829.173 kb on + strand, within nfeF at 1829.173 kb on + strand, within nfeF at 1829.173 kb on + strand, within nfeF at 1829.173 kb on + strand, within nfeF at 1829.174 kb on - strand, within nfeF at 1829.293 kb on + strand, within nfeF at 1829.294 kb on - strand, within nfeF at 1829.316 kb on + strand, within nfeF at 1829.392 kb on + strand, within nfeF at 1829.393 kb on - strand, within nfeF at 1829.393 kb on - strand, within nfeF at 1829.418 kb on + strand, within nfeF at 1829.419 kb on - strand, within nfeF at 1829.492 kb on - strand at 1829.515 kb on + strand at 1829.556 kb on + strand at 1829.556 kb on + strand at 1829.556 kb on + strand at 1829.557 kb on - strand at 1829.557 kb on - strand at 1829.558 kb on + strand at 1829.559 kb on - strand at 1829.559 kb on - strand at 1829.602 kb on - strand, within Ile2_trna at 1829.629 kb on + strand, within Ile2_trna at 1829.650 kb on + strand at 1829.652 kb on + strand at 1829.657 kb on + strand at 1829.682 kb on + strand at 1829.730 kb on - strand at 1829.730 kb on - strand at 1829.747 kb on + strand at 1829.748 kb on - strand at 1829.757 kb on + strand at 1829.757 kb on + strand at 1829.758 kb on - strand at 1829.761 kb on + strand at 1829.761 kb on + strand at 1829.883 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.886 kb on - strand, within mug at 1829.899 kb on + strand, within mug at 1829.945 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.193 kb on - strand, within mug at 1830.324 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,827,746 + -0.4 1,827,757 + +0.8 1,827,766 + +0.1 1,827,767 - -1.6 1,827,835 - +0.7 1,827,851 - +1.4 1,827,908 - nfeR MCAODC_09150 0.12 +0.8 1,827,908 - nfeR MCAODC_09150 0.12 +0.5 1,827,927 - nfeR MCAODC_09150 0.15 +0.8 1,828,094 - nfeR MCAODC_09150 0.42 -2.4 1,828,155 + nfeR MCAODC_09150 0.52 -0.5 1,828,180 - nfeR MCAODC_09150 0.56 -0.1 1,828,430 - +1.1 1,828,430 - -0.6 1,828,558 + +1.8 1,828,558 + -1.7 1,828,597 + -0.4 1,828,597 + -0.9 1,828,597 + +0.4 1,828,597 + -0.2 1,828,597 + -0.9 1,828,597 + +3.1 1,828,598 - -0.6 1,828,637 + -2.5 1,828,638 - +0.4 1,828,643 + -1.2 1,828,643 + +0.9 1,828,644 - -0.7 1,828,644 - -0.3 1,828,670 - -0.5 1,828,714 + -0.1 1,828,825 - nfeF MCAODC_09155 0.11 +0.5 1,828,825 - nfeF MCAODC_09155 0.11 -0.3 1,828,878 + nfeF MCAODC_09155 0.18 -3.7 1,828,879 - nfeF MCAODC_09155 0.18 +0.9 1,829,006 - nfeF MCAODC_09155 0.34 +0.4 1,829,006 - nfeF MCAODC_09155 0.34 -1.2 1,829,007 + nfeF MCAODC_09155 0.34 +0.9 1,829,008 - nfeF MCAODC_09155 0.35 +1.3 1,829,027 + nfeF MCAODC_09155 0.37 -0.3 1,829,028 - nfeF MCAODC_09155 0.37 +0.1 1,829,028 - nfeF MCAODC_09155 0.37 -0.4 1,829,056 - nfeF MCAODC_09155 0.41 +1.4 1,829,165 - nfeF MCAODC_09155 0.55 +1.5 1,829,173 + nfeF MCAODC_09155 0.56 -0.1 1,829,173 + nfeF MCAODC_09155 0.56 -2.1 1,829,173 + nfeF MCAODC_09155 0.56 -0.6 1,829,173 + nfeF MCAODC_09155 0.56 +0.3 1,829,174 - nfeF MCAODC_09155 0.56 +0.6 1,829,293 + nfeF MCAODC_09155 0.72 -0.3 1,829,294 - nfeF MCAODC_09155 0.72 -1.8 1,829,316 + nfeF MCAODC_09155 0.75 +0.8 1,829,392 + nfeF MCAODC_09155 0.85 +0.2 1,829,393 - nfeF MCAODC_09155 0.85 -2.8 1,829,393 - nfeF MCAODC_09155 0.85 -0.6 1,829,418 + nfeF MCAODC_09155 0.88 -3.0 1,829,419 - nfeF MCAODC_09155 0.88 -0.0 1,829,492 - +1.3 1,829,515 + -0.0 1,829,556 + -1.6 1,829,556 + +1.0 1,829,556 + +0.6 1,829,557 - +0.4 1,829,557 - +0.1 1,829,558 + +1.5 1,829,559 - +0.3 1,829,559 - -2.3 1,829,602 - Ile2_trna MCAODC_09160 0.53 +0.1 1,829,629 + Ile2_trna MCAODC_09160 0.88 +1.2 1,829,650 + -1.8 1,829,652 + +0.5 1,829,657 + +0.5 1,829,682 + -0.5 1,829,730 - -0.6 1,829,730 - +0.8 1,829,747 + -0.8 1,829,748 - +0.5 1,829,757 + +0.9 1,829,757 + -0.5 1,829,758 - +0.4 1,829,761 + +0.1 1,829,761 + -1.1 1,829,883 + mug MCAODC_09165 0.24 -0.0 1,829,885 + mug MCAODC_09165 0.24 -2.6 1,829,885 + mug MCAODC_09165 0.24 +0.5 1,829,885 + mug MCAODC_09165 0.24 -0.0 1,829,886 - mug MCAODC_09165 0.24 -1.9 1,829,899 + mug MCAODC_09165 0.27 -1.4 1,829,945 + mug MCAODC_09165 0.36 +1.2 1,830,095 + mug MCAODC_09165 0.66 -1.0 1,830,095 + mug MCAODC_09165 0.66 -1.5 1,830,095 + mug MCAODC_09165 0.66 +0.0 1,830,193 - mug MCAODC_09165 0.85 -2.9 1,830,324 + -0.9
Or see this region's nucleotide sequence