Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysE and trmL are separated by 52 nucleotides trmL and lldD are separated by 185 nucleotides lldD and lldR overlap by 4 nucleotides lldR and lldP overlap by 1 nucleotides
MCAODC_06270: cysE - serine O-acetyltransferase, at 1,256,777 to 1,257,598
cysE
MCAODC_06275: trmL - tRNA (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine (34)-2'-O)-methyltransferase TrmL, at 1,257,651 to 1,258,124
trmL
MCAODC_06280: lldD - quinone-dependent L-lactate dehydrogenase, at 1,258,310 to 1,259,500
lldD
MCAODC_06285: lldR - transcriptional regulator LldR, at 1,259,497 to 1,260,273
lldR
MCAODC_06290: lldP - L-lactate permease, at 1,260,273 to 1,261,928
lldP
Position (kb) 1258
1259
1260 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1257.511 kb on + strand, within cysE at 1257.563 kb on - strand at 1257.563 kb on - strand at 1257.565 kb on + strand at 1257.565 kb on + strand at 1257.566 kb on - strand at 1257.571 kb on + strand at 1257.571 kb on + strand at 1257.571 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.596 kb on + strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.597 kb on - strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.626 kb on + strand at 1257.627 kb on - strand at 1257.627 kb on - strand at 1257.627 kb on - strand at 1257.634 kb on + strand at 1257.635 kb on - strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.643 kb on + strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.644 kb on - strand at 1257.648 kb on + strand at 1257.649 kb on - strand at 1257.649 kb on - strand at 1257.649 kb on - strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.652 kb on + strand at 1257.653 kb on - strand at 1257.653 kb on - strand at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.700 kb on + strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.701 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.839 kb on - strand, within trmL at 1257.854 kb on - strand, within trmL at 1257.854 kb on - strand, within trmL at 1257.859 kb on - strand, within trmL at 1257.878 kb on + strand, within trmL at 1257.879 kb on - strand, within trmL at 1257.973 kb on + strand, within trmL at 1257.974 kb on - strand, within trmL at 1258.035 kb on - strand, within trmL at 1258.052 kb on + strand, within trmL at 1258.052 kb on + strand, within trmL at 1258.073 kb on + strand, within trmL at 1258.073 kb on + strand, within trmL at 1258.074 kb on - strand, within trmL at 1258.074 kb on - strand, within trmL at 1258.082 kb on + strand at 1258.083 kb on - strand at 1258.105 kb on + strand at 1258.105 kb on + strand at 1258.106 kb on - strand at 1258.106 kb on - strand at 1258.110 kb on + strand at 1258.110 kb on + strand at 1258.119 kb on + strand at 1258.120 kb on - strand at 1258.124 kb on + strand at 1258.125 kb on - strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.245 kb on + strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.246 kb on - strand at 1258.249 kb on + strand at 1258.249 kb on + strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.250 kb on - strand at 1258.311 kb on + strand at 1258.391 kb on - strand at 1258.457 kb on + strand, within lldD at 1258.465 kb on + strand, within lldD at 1258.466 kb on - strand, within lldD at 1258.466 kb on - strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.469 kb on + strand, within lldD at 1258.470 kb on - strand, within lldD at 1258.470 kb on - strand, within lldD at 1258.500 kb on + strand, within lldD at 1258.523 kb on + strand, within lldD at 1258.523 kb on + strand, within lldD at 1258.550 kb on - strand, within lldD at 1258.550 kb on - strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.652 kb on + strand, within lldD at 1258.653 kb on - strand, within lldD at 1258.681 kb on + strand, within lldD at 1258.689 kb on - strand, within lldD at 1258.689 kb on - strand, within lldD at 1258.712 kb on + strand, within lldD at 1258.842 kb on + strand, within lldD at 1258.842 kb on + strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.843 kb on - strand, within lldD at 1258.872 kb on + strand, within lldD at 1258.882 kb on + strand, within lldD at 1258.882 kb on + strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.886 kb on - strand, within lldD at 1258.889 kb on + strand, within lldD at 1258.889 kb on + strand, within lldD at 1258.890 kb on - strand, within lldD at 1259.030 kb on - strand, within lldD at 1259.030 kb on - strand, within lldD at 1259.093 kb on - strand, within lldD at 1259.111 kb on + strand, within lldD at 1259.111 kb on + strand, within lldD at 1259.112 kb on - strand, within lldD at 1259.112 kb on - strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.115 kb on + strand, within lldD at 1259.116 kb on - strand, within lldD at 1259.116 kb on - strand, within lldD at 1259.116 kb on - strand, within lldD at 1259.250 kb on + strand, within lldD at 1259.250 kb on + strand, within lldD at 1259.250 kb on + strand, within lldD at 1259.250 kb on + strand, within lldD at 1259.250 kb on + strand, within lldD at 1259.251 kb on - strand, within lldD at 1259.251 kb on - strand, within lldD at 1259.251 kb on - strand, within lldD at 1259.251 kb on - strand, within lldD at 1259.251 kb on - strand, within lldD at 1259.327 kb on + strand, within lldD at 1259.328 kb on - strand, within lldD at 1259.350 kb on + strand, within lldD at 1259.350 kb on + strand, within lldD at 1259.351 kb on - strand, within lldD at 1259.413 kb on - strand at 1259.413 kb on - strand at 1259.428 kb on + strand at 1259.428 kb on + strand at 1259.428 kb on + strand at 1259.428 kb on + strand at 1259.429 kb on - strand at 1259.429 kb on - strand at 1259.430 kb on + strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.431 kb on - strand at 1259.494 kb on + strand at 1259.494 kb on + strand at 1259.494 kb on + strand at 1259.495 kb on - strand at 1259.495 kb on - strand at 1259.495 kb on - strand at 1259.526 kb on + strand at 1259.526 kb on + strand at 1259.526 kb on + strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.527 kb on - strand at 1259.548 kb on - strand at 1259.607 kb on + strand, within lldR at 1259.607 kb on + strand, within lldR at 1259.608 kb on - strand, within lldR at 1259.608 kb on - strand, within lldR at 1259.608 kb on - strand, within lldR at 1259.629 kb on - strand, within lldR at 1259.717 kb on + strand, within lldR at 1259.717 kb on + strand, within lldR at 1259.718 kb on - strand, within lldR at 1259.718 kb on - strand, within lldR at 1259.733 kb on + strand, within lldR at 1259.796 kb on + strand, within lldR at 1259.796 kb on + strand, within lldR at 1259.796 kb on + strand, within lldR at 1259.796 kb on + strand, within lldR at 1259.796 kb on + strand, within lldR at 1259.797 kb on - strand, within lldR at 1259.797 kb on - strand, within lldR at 1259.797 kb on - strand, within lldR at 1259.797 kb on - strand, within lldR at 1259.797 kb on - strand, within lldR at 1259.866 kb on + strand, within lldR at 1259.958 kb on - strand, within lldR at 1259.985 kb on - strand, within lldR at 1260.138 kb on + strand, within lldR at 1260.138 kb on + strand, within lldR at 1260.138 kb on + strand, within lldR at 1260.139 kb on - strand, within lldR at 1260.139 kb on - strand, within lldR at 1260.139 kb on - strand, within lldR at 1260.145 kb on + strand, within lldR at 1260.274 kb on + strand at 1260.298 kb on + strand at 1260.298 kb on + strand at 1260.299 kb on - strand at 1260.299 kb on - strand at 1260.299 kb on - strand at 1260.329 kb on + strand at 1260.330 kb on - strand at 1260.330 kb on - strand at 1260.419 kb on + strand at 1260.419 kb on + strand at 1260.419 kb on + strand at 1260.420 kb on - strand at 1260.420 kb on - strand at 1260.420 kb on - strand at 1260.461 kb on + strand, within lldP
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,257,511 + cysE MCAODC_06270 0.89 +0.2 1,257,563 - -0.6 1,257,563 - -3.2 1,257,565 + +0.4 1,257,565 + +0.0 1,257,566 - -1.3 1,257,571 + -0.4 1,257,571 + +0.5 1,257,571 + -0.0 1,257,596 + -0.1 1,257,596 + -3.8 1,257,596 + -0.5 1,257,596 + -0.4 1,257,596 + +0.2 1,257,596 + +1.1 1,257,596 + +1.0 1,257,596 + +0.0 1,257,596 + +1.3 1,257,596 + +1.3 1,257,597 - +0.4 1,257,597 - +0.8 1,257,597 - +1.0 1,257,597 - +2.0 1,257,597 - +0.8 1,257,597 - +0.4 1,257,597 - +1.1 1,257,626 + -0.5 1,257,626 + -1.1 1,257,626 + -1.1 1,257,626 + -0.3 1,257,627 - +1.6 1,257,627 - +0.6 1,257,627 - -0.6 1,257,634 + +0.3 1,257,635 - -3.0 1,257,643 + -1.1 1,257,643 + +0.5 1,257,643 + +0.4 1,257,643 + -0.0 1,257,644 - -0.5 1,257,644 - -3.9 1,257,644 - -0.7 1,257,644 - +0.8 1,257,644 - -0.2 1,257,644 - +0.4 1,257,648 + -0.7 1,257,649 - +1.0 1,257,649 - -3.2 1,257,649 - -0.7 1,257,652 + -2.9 1,257,652 + -3.2 1,257,652 + +0.3 1,257,652 + +0.5 1,257,653 - +0.2 1,257,653 - +0.6 1,257,700 + trmL MCAODC_06275 0.10 +1.6 1,257,700 + trmL MCAODC_06275 0.10 +0.8 1,257,700 + trmL MCAODC_06275 0.10 +1.9 1,257,700 + trmL MCAODC_06275 0.10 -0.5 1,257,700 + trmL MCAODC_06275 0.10 +0.8 1,257,701 - trmL MCAODC_06275 0.11 -0.0 1,257,701 - trmL MCAODC_06275 0.11 +1.0 1,257,701 - trmL MCAODC_06275 0.11 -1.8 1,257,701 - trmL MCAODC_06275 0.11 +1.4 1,257,839 - trmL MCAODC_06275 0.40 -1.0 1,257,839 - trmL MCAODC_06275 0.40 +1.3 1,257,839 - trmL MCAODC_06275 0.40 -1.0 1,257,854 - trmL MCAODC_06275 0.43 -0.7 1,257,854 - trmL MCAODC_06275 0.43 -3.3 1,257,859 - trmL MCAODC_06275 0.44 -2.7 1,257,878 + trmL MCAODC_06275 0.48 +0.5 1,257,879 - trmL MCAODC_06275 0.48 +0.6 1,257,973 + trmL MCAODC_06275 0.68 +1.3 1,257,974 - trmL MCAODC_06275 0.68 -0.5 1,258,035 - trmL MCAODC_06275 0.81 +0.2 1,258,052 + trmL MCAODC_06275 0.85 +0.8 1,258,052 + trmL MCAODC_06275 0.85 -0.7 1,258,073 + trmL MCAODC_06275 0.89 +0.6 1,258,073 + trmL MCAODC_06275 0.89 +0.5 1,258,074 - trmL MCAODC_06275 0.89 +2.0 1,258,074 - trmL MCAODC_06275 0.89 -0.0 1,258,082 + +0.1 1,258,083 - +0.0 1,258,105 + -0.5 1,258,105 + +0.8 1,258,106 - -0.5 1,258,106 - -0.3 1,258,110 + +1.1 1,258,110 + -1.3 1,258,119 + +0.3 1,258,120 - -2.3 1,258,124 + +1.1 1,258,125 - -0.2 1,258,245 + -0.7 1,258,245 + +0.5 1,258,245 + +1.8 1,258,245 + -0.6 1,258,246 - +0.0 1,258,246 - +0.8 1,258,246 - -2.0 1,258,246 - +0.2 1,258,249 + -0.9 1,258,249 + +0.1 1,258,250 - -0.8 1,258,250 - +0.6 1,258,250 - +1.6 1,258,250 - +0.2 1,258,250 - -2.9 1,258,250 - -1.0 1,258,311 + +2.3 1,258,391 - +0.6 1,258,457 + lldD MCAODC_06280 0.12 -0.6 1,258,465 + lldD MCAODC_06280 0.13 +2.3 1,258,466 - lldD MCAODC_06280 0.13 -0.2 1,258,466 - lldD MCAODC_06280 0.13 +1.4 1,258,469 + lldD MCAODC_06280 0.13 +0.4 1,258,469 + lldD MCAODC_06280 0.13 +1.2 1,258,469 + lldD MCAODC_06280 0.13 +1.0 1,258,470 - lldD MCAODC_06280 0.13 -0.1 1,258,470 - lldD MCAODC_06280 0.13 -1.6 1,258,500 + lldD MCAODC_06280 0.16 +3.3 1,258,523 + lldD MCAODC_06280 0.18 -0.6 1,258,523 + lldD MCAODC_06280 0.18 +1.9 1,258,550 - lldD MCAODC_06280 0.20 +0.6 1,258,550 - lldD MCAODC_06280 0.20 -0.8 1,258,652 + lldD MCAODC_06280 0.29 +0.4 1,258,652 + lldD MCAODC_06280 0.29 +1.2 1,258,652 + lldD MCAODC_06280 0.29 -0.2 1,258,652 + lldD MCAODC_06280 0.29 +1.1 1,258,653 - lldD MCAODC_06280 0.29 -1.5 1,258,681 + lldD MCAODC_06280 0.31 +0.3 1,258,689 - lldD MCAODC_06280 0.32 +0.3 1,258,689 - lldD MCAODC_06280 0.32 -2.8 1,258,712 + lldD MCAODC_06280 0.34 -2.6 1,258,842 + lldD MCAODC_06280 0.45 -0.6 1,258,842 + lldD MCAODC_06280 0.45 -2.0 1,258,843 - lldD MCAODC_06280 0.45 +0.8 1,258,843 - lldD MCAODC_06280 0.45 -0.3 1,258,843 - lldD MCAODC_06280 0.45 +0.5 1,258,843 - lldD MCAODC_06280 0.45 +0.8 1,258,872 + lldD MCAODC_06280 0.47 +0.2 1,258,882 + lldD MCAODC_06280 0.48 +0.3 1,258,882 + lldD MCAODC_06280 0.48 -0.8 1,258,886 - lldD MCAODC_06280 0.48 -1.4 1,258,886 - lldD MCAODC_06280 0.48 +1.0 1,258,886 - lldD MCAODC_06280 0.48 +0.3 1,258,889 + lldD MCAODC_06280 0.49 -1.5 1,258,889 + lldD MCAODC_06280 0.49 -1.4 1,258,890 - lldD MCAODC_06280 0.49 +2.3 1,259,030 - lldD MCAODC_06280 0.60 +0.1 1,259,030 - lldD MCAODC_06280 0.60 -1.9 1,259,093 - lldD MCAODC_06280 0.66 +0.2 1,259,111 + lldD MCAODC_06280 0.67 -0.3 1,259,111 + lldD MCAODC_06280 0.67 +1.1 1,259,112 - lldD MCAODC_06280 0.67 -0.2 1,259,112 - lldD MCAODC_06280 0.67 -0.9 1,259,115 + lldD MCAODC_06280 0.68 +0.4 1,259,115 + lldD MCAODC_06280 0.68 -0.9 1,259,115 + lldD MCAODC_06280 0.68 +0.1 1,259,116 - lldD MCAODC_06280 0.68 -0.7 1,259,116 - lldD MCAODC_06280 0.68 -1.0 1,259,116 - lldD MCAODC_06280 0.68 -0.1 1,259,250 + lldD MCAODC_06280 0.79 -0.2 1,259,250 + lldD MCAODC_06280 0.79 +1.2 1,259,250 + lldD MCAODC_06280 0.79 +0.8 1,259,250 + lldD MCAODC_06280 0.79 -0.6 1,259,250 + lldD MCAODC_06280 0.79 +0.5 1,259,251 - lldD MCAODC_06280 0.79 -0.8 1,259,251 - lldD MCAODC_06280 0.79 +1.5 1,259,251 - lldD MCAODC_06280 0.79 +0.4 1,259,251 - lldD MCAODC_06280 0.79 +0.3 1,259,251 - lldD MCAODC_06280 0.79 +0.9 1,259,327 + lldD MCAODC_06280 0.85 +0.3 1,259,328 - lldD MCAODC_06280 0.85 -0.6 1,259,350 + lldD MCAODC_06280 0.87 -0.4 1,259,350 + lldD MCAODC_06280 0.87 -0.2 1,259,351 - lldD MCAODC_06280 0.87 -1.0 1,259,413 - +1.0 1,259,413 - +0.1 1,259,428 + +0.5 1,259,428 + -0.4 1,259,428 + -0.0 1,259,428 + -1.9 1,259,429 - +0.5 1,259,429 - +0.4 1,259,430 + -0.6 1,259,431 - -0.5 1,259,431 - +1.0 1,259,431 - +0.0 1,259,431 - +0.7 1,259,431 - +0.2 1,259,431 - +0.0 1,259,431 - -1.3 1,259,494 + +0.8 1,259,494 + +0.8 1,259,494 + +0.4 1,259,495 - -0.9 1,259,495 - +0.6 1,259,495 - +0.2 1,259,526 + +0.6 1,259,526 + +0.9 1,259,526 + -0.4 1,259,527 - +0.8 1,259,527 - -2.7 1,259,527 - +0.9 1,259,527 - -0.4 1,259,527 - -2.1 1,259,527 - +0.7 1,259,527 - +0.8 1,259,548 - +0.9 1,259,607 + lldR MCAODC_06285 0.14 -1.4 1,259,607 + lldR MCAODC_06285 0.14 -0.6 1,259,608 - lldR MCAODC_06285 0.14 -3.1 1,259,608 - lldR MCAODC_06285 0.14 -0.2 1,259,608 - lldR MCAODC_06285 0.14 +0.8 1,259,629 - lldR MCAODC_06285 0.17 +1.5 1,259,717 + lldR MCAODC_06285 0.28 +0.2 1,259,717 + lldR MCAODC_06285 0.28 +1.1 1,259,718 - lldR MCAODC_06285 0.28 +0.0 1,259,718 - lldR MCAODC_06285 0.28 +0.5 1,259,733 + lldR MCAODC_06285 0.30 +0.4 1,259,796 + lldR MCAODC_06285 0.38 -0.4 1,259,796 + lldR MCAODC_06285 0.38 -0.6 1,259,796 + lldR MCAODC_06285 0.38 +0.9 1,259,796 + lldR MCAODC_06285 0.38 +0.5 1,259,796 + lldR MCAODC_06285 0.38 -3.3 1,259,797 - lldR MCAODC_06285 0.39 -2.0 1,259,797 - lldR MCAODC_06285 0.39 -0.6 1,259,797 - lldR MCAODC_06285 0.39 -2.6 1,259,797 - lldR MCAODC_06285 0.39 -3.4 1,259,797 - lldR MCAODC_06285 0.39 -0.4 1,259,866 + lldR MCAODC_06285 0.47 -0.4 1,259,958 - lldR MCAODC_06285 0.59 +2.1 1,259,985 - lldR MCAODC_06285 0.63 +0.7 1,260,138 + lldR MCAODC_06285 0.82 -0.5 1,260,138 + lldR MCAODC_06285 0.82 -1.0 1,260,138 + lldR MCAODC_06285 0.82 +0.2 1,260,139 - lldR MCAODC_06285 0.83 +0.9 1,260,139 - lldR MCAODC_06285 0.83 -3.0 1,260,139 - lldR MCAODC_06285 0.83 +0.5 1,260,145 + lldR MCAODC_06285 0.83 -1.8 1,260,274 + -0.7 1,260,298 + +0.5 1,260,298 + +0.0 1,260,299 - -0.2 1,260,299 - -2.4 1,260,299 - +1.5 1,260,329 + +0.1 1,260,330 - +0.6 1,260,330 - +1.4 1,260,419 + +0.3 1,260,419 + +0.1 1,260,419 + +2.5 1,260,420 - -0.7 1,260,420 - +0.9 1,260,420 - -1.2 1,260,461 + lldP MCAODC_06290 0.11 -3.3
Or see this region's nucleotide sequence