Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recG and recG are separated by 5 nucleotides recG and spoT are separated by 39 nucleotides spoT and rpoZ are separated by 18 nucleotides rpoZ and gmk are separated by 54 nucleotides
MCAODC_06055: recG - ATP-dependent DNA helicase RecG, at 1,215,018 to 1,217,099
recG
MCAODC_06060: recG - ATP-dependent DNA helicase RecG, at 1,217,105 to 1,217,761
recG
MCAODC_06065: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 1,217,801 to 1,219,909
spoT
MCAODC_06070: rpoZ - DNA-directed RNA polymerase subunit omega, at 1,219,928 to 1,220,203
rpoZ
MCAODC_06075: gmk - guanylate kinase, at 1,220,258 to 1,220,881
gmk
Position (kb)
1217
1218
1219
1220 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1216.883 kb on + strand, within recG at 1216.884 kb on - strand, within recG at 1216.918 kb on + strand at 1216.932 kb on + strand at 1216.932 kb on + strand at 1216.933 kb on - strand at 1216.949 kb on - strand at 1216.969 kb on + strand at 1216.986 kb on + strand at 1216.986 kb on + strand at 1216.986 kb on + strand at 1216.986 kb on + strand at 1216.987 kb on - strand at 1216.987 kb on - strand at 1216.987 kb on - strand at 1216.987 kb on - strand at 1217.006 kb on + strand at 1217.007 kb on - strand at 1217.033 kb on + strand at 1217.036 kb on + strand at 1217.037 kb on - strand at 1217.082 kb on + strand at 1217.082 kb on + strand at 1217.083 kb on - strand at 1217.107 kb on - strand at 1217.107 kb on - strand at 1217.107 kb on - strand at 1217.107 kb on - strand at 1217.107 kb on - strand at 1217.122 kb on + strand at 1217.122 kb on + strand at 1217.122 kb on + strand at 1217.122 kb on + strand at 1217.123 kb on - strand at 1217.123 kb on - strand at 1217.123 kb on - strand at 1217.123 kb on - strand at 1217.126 kb on - strand at 1217.179 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.284 kb on - strand, within recG at 1217.319 kb on + strand, within recG at 1217.466 kb on + strand, within recG at 1217.466 kb on + strand, within recG at 1217.467 kb on - strand, within recG at 1217.593 kb on + strand, within recG at 1217.593 kb on + strand, within recG at 1217.596 kb on + strand, within recG at 1217.633 kb on - strand, within recG at 1217.660 kb on + strand, within recG at 1217.661 kb on - strand, within recG at 1217.661 kb on - strand, within recG at 1217.661 kb on - strand, within recG at 1217.686 kb on + strand, within recG at 1217.687 kb on - strand, within recG at 1217.687 kb on - strand, within recG at 1217.702 kb on - strand at 1217.794 kb on + strand at 1217.794 kb on + strand at 1217.795 kb on - strand at 1217.795 kb on - strand at 1217.795 kb on - strand at 1217.825 kb on - strand at 1217.825 kb on - strand at 1217.861 kb on - strand at 1217.936 kb on - strand at 1217.951 kb on - strand at 1217.968 kb on + strand at 1217.968 kb on + strand at 1218.073 kb on + strand, within spoT at 1218.110 kb on - strand, within spoT at 1218.389 kb on - strand, within spoT at 1218.461 kb on + strand, within spoT at 1220.145 kb on - strand, within rpoZ at 1220.175 kb on - strand, within rpoZ at 1220.193 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.208 kb on - strand at 1220.895 kb on - strand at 1220.895 kb on - strand at 1220.895 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 1,216,883 + recG MCAODC_06055 0.90 -1.2 1,216,884 - recG MCAODC_06055 0.90 +0.3 1,216,918 + -0.3 1,216,932 + -0.7 1,216,932 + -0.1 1,216,933 - -1.8 1,216,949 - +0.6 1,216,969 + -0.5 1,216,986 + -0.5 1,216,986 + -0.3 1,216,986 + -1.8 1,216,986 + -4.1 1,216,987 - -3.6 1,216,987 - +0.5 1,216,987 - -0.7 1,216,987 - -1.1 1,217,006 + -0.5 1,217,007 - -1.0 1,217,033 + -0.1 1,217,036 + -0.4 1,217,037 - +2.5 1,217,082 + -1.4 1,217,082 + -2.4 1,217,083 - -0.6 1,217,107 - -0.3 1,217,107 - -1.1 1,217,107 - +0.2 1,217,107 - -3.2 1,217,107 - +0.3 1,217,122 + +0.8 1,217,122 + -2.9 1,217,122 + +0.6 1,217,122 + -1.0 1,217,123 - -2.4 1,217,123 - -0.3 1,217,123 - +1.3 1,217,123 - -0.1 1,217,126 - +0.0 1,217,179 - recG MCAODC_06060 0.11 -0.0 1,217,284 - recG MCAODC_06060 0.27 +0.9 1,217,284 - recG MCAODC_06060 0.27 -0.3 1,217,284 - recG MCAODC_06060 0.27 +0.8 1,217,284 - recG MCAODC_06060 0.27 +1.0 1,217,284 - recG MCAODC_06060 0.27 -0.2 1,217,284 - recG MCAODC_06060 0.27 -0.7 1,217,284 - recG MCAODC_06060 0.27 +0.0 1,217,319 + recG MCAODC_06060 0.33 -0.8 1,217,466 + recG MCAODC_06060 0.55 +1.9 1,217,466 + recG MCAODC_06060 0.55 +0.3 1,217,467 - recG MCAODC_06060 0.55 -1.5 1,217,593 + recG MCAODC_06060 0.74 +0.1 1,217,593 + recG MCAODC_06060 0.74 +1.3 1,217,596 + recG MCAODC_06060 0.75 -0.5 1,217,633 - recG MCAODC_06060 0.80 -2.4 1,217,660 + recG MCAODC_06060 0.84 +0.7 1,217,661 - recG MCAODC_06060 0.85 +1.0 1,217,661 - recG MCAODC_06060 0.85 -1.0 1,217,661 - recG MCAODC_06060 0.85 +2.2 1,217,686 + recG MCAODC_06060 0.88 -1.4 1,217,687 - recG MCAODC_06060 0.89 +1.3 1,217,687 - recG MCAODC_06060 0.89 -0.4 1,217,702 - -0.7 1,217,794 + +1.1 1,217,794 + -2.4 1,217,795 - -0.7 1,217,795 - -2.5 1,217,795 - -3.3 1,217,825 - -2.4 1,217,825 - -1.4 1,217,861 - -0.8 1,217,936 - -1.8 1,217,951 - +0.1 1,217,968 + +1.0 1,217,968 + -2.1 1,218,073 + spoT MCAODC_06065 0.13 +2.0 1,218,110 - spoT MCAODC_06065 0.15 -2.4 1,218,389 - spoT MCAODC_06065 0.28 -4.4 1,218,461 + spoT MCAODC_06065 0.31 +0.2 1,220,145 - rpoZ MCAODC_06070 0.79 -1.5 1,220,175 - rpoZ MCAODC_06070 0.89 -1.0 1,220,193 - -0.8 1,220,208 - -0.1 1,220,208 - +0.6 1,220,208 - +0.1 1,220,208 - +0.7 1,220,895 - -0.2 1,220,895 - +0.7 1,220,895 - +1.9
Or see this region's nucleotide sequence