Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rapA and rluA are separated by 11 nucleotides rluA and espY1 are separated by 417 nucleotides
MCAODC_01315: rapA - RNA polymerase-associated protein RapA, at 249,014 to 251,920
rapA
MCAODC_01320: rluA - bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA, at 251,932 to 252,591
rluA
MCAODC_01325: espY1 - Type III secretion system effector EspY1, at 253,009 to 253,746
espY1
Position (kb) 251
252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 250.936 kb on + strand, within rapA at 250.940 kb on + strand, within rapA at 250.940 kb on + strand, within rapA at 250.941 kb on - strand, within rapA at 250.941 kb on - strand, within rapA at 250.941 kb on - strand, within rapA at 250.950 kb on + strand, within rapA at 250.950 kb on + strand, within rapA at 250.950 kb on + strand, within rapA at 250.950 kb on + strand, within rapA at 250.950 kb on + strand, within rapA at 250.951 kb on - strand, within rapA at 250.952 kb on + strand, within rapA at 250.952 kb on + strand, within rapA at 250.952 kb on + strand, within rapA at 250.953 kb on - strand, within rapA at 250.966 kb on + strand, within rapA at 250.967 kb on - strand, within rapA at 250.970 kb on + strand, within rapA at 250.970 kb on + strand, within rapA at 250.970 kb on + strand, within rapA at 250.971 kb on - strand, within rapA at 250.971 kb on - strand, within rapA at 250.971 kb on - strand, within rapA at 251.248 kb on + strand, within rapA at 251.248 kb on + strand, within rapA at 251.249 kb on - strand, within rapA at 251.342 kb on - strand, within rapA at 251.344 kb on + strand, within rapA at 251.344 kb on + strand, within rapA at 251.471 kb on + strand, within rapA at 251.471 kb on + strand, within rapA at 251.539 kb on + strand, within rapA at 251.539 kb on + strand, within rapA at 251.539 kb on + strand, within rapA at 251.539 kb on + strand, within rapA at 251.539 kb on + strand, within rapA at 251.540 kb on - strand, within rapA at 251.540 kb on - strand, within rapA at 251.540 kb on - strand, within rapA at 251.540 kb on - strand, within rapA at 251.540 kb on - strand, within rapA at 251.540 kb on - strand, within rapA at 251.608 kb on + strand, within rapA at 251.608 kb on + strand, within rapA at 251.671 kb on + strand at 251.850 kb on + strand at 251.850 kb on + strand at 251.850 kb on + strand at 251.850 kb on + strand at 251.851 kb on - strand at 251.908 kb on - strand at 251.919 kb on - strand at 251.976 kb on + strand at 251.983 kb on + strand at 251.984 kb on - strand at 251.997 kb on + strand at 251.997 kb on + strand at 251.997 kb on + strand at 251.997 kb on + strand at 251.998 kb on - strand, within rluA at 251.998 kb on - strand, within rluA at 251.998 kb on - strand, within rluA at 251.998 kb on - strand, within rluA at 251.998 kb on - strand, within rluA at 252.000 kb on + strand, within rluA at 252.001 kb on - strand, within rluA at 252.001 kb on - strand, within rluA at 252.001 kb on - strand, within rluA at 252.147 kb on - strand, within rluA at 252.179 kb on + strand, within rluA at 252.179 kb on + strand, within rluA at 252.217 kb on + strand, within rluA at 252.217 kb on + strand, within rluA at 252.218 kb on - strand, within rluA at 252.218 kb on - strand, within rluA at 252.218 kb on - strand, within rluA at 252.218 kb on - strand, within rluA at 252.316 kb on + strand, within rluA at 252.361 kb on + strand, within rluA at 252.362 kb on - strand, within rluA at 252.362 kb on - strand, within rluA at 252.433 kb on - strand, within rluA at 252.433 kb on - strand, within rluA at 252.473 kb on - strand, within rluA at 252.515 kb on + strand, within rluA at 252.515 kb on + strand, within rluA at 252.515 kb on + strand, within rluA at 252.516 kb on - strand, within rluA at 252.516 kb on - strand, within rluA at 252.551 kb on - strand at 252.558 kb on + strand at 252.558 kb on + strand at 252.558 kb on + strand at 252.558 kb on + strand at 252.558 kb on + strand at 252.559 kb on - strand at 252.561 kb on + strand at 252.562 kb on - strand at 252.571 kb on - strand at 252.607 kb on + strand at 252.607 kb on + strand at 252.607 kb on + strand at 252.610 kb on - strand at 252.610 kb on - strand at 252.621 kb on - strand at 252.636 kb on + strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.641 kb on - strand at 252.641 kb on - strand at 252.641 kb on - strand at 252.641 kb on - strand at 252.641 kb on - strand at 252.641 kb on - strand at 252.702 kb on + strand at 252.779 kb on + strand at 252.779 kb on + strand at 252.779 kb on + strand at 252.780 kb on - strand at 252.781 kb on + strand at 252.782 kb on - strand at 252.804 kb on - strand at 252.823 kb on - strand at 252.834 kb on - strand at 252.838 kb on - strand at 252.879 kb on + strand at 252.899 kb on + strand at 252.911 kb on + strand at 252.912 kb on - strand at 252.912 kb on - strand at 252.912 kb on - strand at 252.933 kb on + strand at 252.934 kb on - strand at 252.996 kb on - strand at 252.996 kb on - strand at 253.000 kb on + strand at 253.001 kb on - strand at 253.018 kb on - strand at 253.033 kb on - strand at 253.047 kb on + strand at 253.048 kb on - strand at 253.107 kb on + strand, within espY1 at 253.108 kb on - strand, within espY1 at 253.108 kb on - strand, within espY1 at 253.112 kb on - strand, within espY1 at 253.121 kb on + strand, within espY1 at 253.122 kb on - strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.123 kb on + strand, within espY1 at 253.124 kb on - strand, within espY1 at 253.124 kb on - strand, within espY1 at 253.209 kb on + strand, within espY1 at 253.209 kb on + strand, within espY1 at 253.271 kb on + strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.272 kb on - strand, within espY1 at 253.281 kb on + strand, within espY1 at 253.300 kb on - strand, within espY1 at 253.336 kb on + strand, within espY1 at 253.369 kb on + strand, within espY1 at 253.408 kb on - strand, within espY1 at 253.408 kb on - strand, within espY1 at 253.477 kb on - strand, within espY1 at 253.518 kb on + strand, within espY1
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 250,936 + rapA MCAODC_01315 0.66 -3.4 250,940 + rapA MCAODC_01315 0.66 -1.7 250,940 + rapA MCAODC_01315 0.66 -1.1 250,941 - rapA MCAODC_01315 0.66 +1.6 250,941 - rapA MCAODC_01315 0.66 -1.2 250,941 - rapA MCAODC_01315 0.66 -1.0 250,950 + rapA MCAODC_01315 0.67 +1.7 250,950 + rapA MCAODC_01315 0.67 -2.1 250,950 + rapA MCAODC_01315 0.67 -0.0 250,950 + rapA MCAODC_01315 0.67 -1.3 250,950 + rapA MCAODC_01315 0.67 -0.5 250,951 - rapA MCAODC_01315 0.67 +0.1 250,952 + rapA MCAODC_01315 0.67 -0.2 250,952 + rapA MCAODC_01315 0.67 +2.3 250,952 + rapA MCAODC_01315 0.67 -0.4 250,953 - rapA MCAODC_01315 0.67 +1.5 250,966 + rapA MCAODC_01315 0.67 -2.4 250,967 - rapA MCAODC_01315 0.67 -1.0 250,970 + rapA MCAODC_01315 0.67 -1.4 250,970 + rapA MCAODC_01315 0.67 +0.5 250,970 + rapA MCAODC_01315 0.67 +0.4 250,971 - rapA MCAODC_01315 0.67 +1.6 250,971 - rapA MCAODC_01315 0.67 -2.5 250,971 - rapA MCAODC_01315 0.67 -0.4 251,248 + rapA MCAODC_01315 0.77 +0.7 251,248 + rapA MCAODC_01315 0.77 +1.1 251,249 - rapA MCAODC_01315 0.77 +1.3 251,342 - rapA MCAODC_01315 0.80 -1.9 251,344 + rapA MCAODC_01315 0.80 +0.4 251,344 + rapA MCAODC_01315 0.80 +0.4 251,471 + rapA MCAODC_01315 0.85 -0.9 251,471 + rapA MCAODC_01315 0.85 -0.0 251,539 + rapA MCAODC_01315 0.87 -1.5 251,539 + rapA MCAODC_01315 0.87 +0.6 251,539 + rapA MCAODC_01315 0.87 -1.4 251,539 + rapA MCAODC_01315 0.87 -0.9 251,539 + rapA MCAODC_01315 0.87 -1.9 251,540 - rapA MCAODC_01315 0.87 -0.4 251,540 - rapA MCAODC_01315 0.87 -0.3 251,540 - rapA MCAODC_01315 0.87 +0.8 251,540 - rapA MCAODC_01315 0.87 -2.2 251,540 - rapA MCAODC_01315 0.87 -1.8 251,540 - rapA MCAODC_01315 0.87 -2.1 251,608 + rapA MCAODC_01315 0.89 +0.5 251,608 + rapA MCAODC_01315 0.89 -0.8 251,671 + +1.3 251,850 + +0.6 251,850 + +1.9 251,850 + -1.4 251,850 + -0.8 251,851 - -1.0 251,908 - +0.0 251,919 - -1.8 251,976 + +0.3 251,983 + +0.5 251,984 - -0.4 251,997 + -0.3 251,997 + +0.6 251,997 + -2.3 251,997 + +0.2 251,998 - rluA MCAODC_01320 0.10 -0.7 251,998 - rluA MCAODC_01320 0.10 -1.6 251,998 - rluA MCAODC_01320 0.10 +0.0 251,998 - rluA MCAODC_01320 0.10 -0.1 251,998 - rluA MCAODC_01320 0.10 -2.7 252,000 + rluA MCAODC_01320 0.10 +0.4 252,001 - rluA MCAODC_01320 0.10 -1.2 252,001 - rluA MCAODC_01320 0.10 +0.6 252,001 - rluA MCAODC_01320 0.10 +0.4 252,147 - rluA MCAODC_01320 0.33 -2.1 252,179 + rluA MCAODC_01320 0.37 -0.9 252,179 + rluA MCAODC_01320 0.37 -1.1 252,217 + rluA MCAODC_01320 0.43 +1.2 252,217 + rluA MCAODC_01320 0.43 +2.8 252,218 - rluA MCAODC_01320 0.43 -2.7 252,218 - rluA MCAODC_01320 0.43 +0.4 252,218 - rluA MCAODC_01320 0.43 -2.6 252,218 - rluA MCAODC_01320 0.43 -0.7 252,316 + rluA MCAODC_01320 0.58 +1.1 252,361 + rluA MCAODC_01320 0.65 -0.3 252,362 - rluA MCAODC_01320 0.65 -2.4 252,362 - rluA MCAODC_01320 0.65 -0.3 252,433 - rluA MCAODC_01320 0.76 +2.1 252,433 - rluA MCAODC_01320 0.76 -1.6 252,473 - rluA MCAODC_01320 0.82 +0.0 252,515 + rluA MCAODC_01320 0.88 +0.3 252,515 + rluA MCAODC_01320 0.88 -2.0 252,515 + rluA MCAODC_01320 0.88 -1.5 252,516 - rluA MCAODC_01320 0.88 -0.2 252,516 - rluA MCAODC_01320 0.88 -2.6 252,551 - -0.3 252,558 + -3.4 252,558 + -0.9 252,558 + +0.1 252,558 + +1.4 252,558 + +0.9 252,559 - -1.0 252,561 + -1.1 252,562 - +0.0 252,571 - -0.5 252,607 + -1.2 252,607 + +0.4 252,607 + -0.7 252,610 - -0.4 252,610 - -0.1 252,621 - +0.4 252,636 + +1.0 252,640 + -0.1 252,640 + -1.3 252,640 + -0.6 252,640 + +1.8 252,640 + -1.7 252,640 + +0.4 252,641 - +0.8 252,641 - +1.3 252,641 - +0.8 252,641 - -1.1 252,641 - +1.7 252,641 - +0.9 252,702 + -1.1 252,779 + -1.1 252,779 + +0.4 252,779 + +1.0 252,780 - -0.9 252,781 + +0.1 252,782 - -0.7 252,804 - -1.5 252,823 - -2.1 252,834 - +0.3 252,838 - -1.0 252,879 + +0.8 252,899 + -0.9 252,911 + +0.9 252,912 - +0.8 252,912 - -1.1 252,912 - -0.4 252,933 + -0.6 252,934 - +0.3 252,996 - -3.0 252,996 - -0.1 253,000 + +1.3 253,001 - +1.6 253,018 - -1.7 253,033 - +0.9 253,047 + +0.7 253,048 - +0.2 253,107 + espY1 MCAODC_01325 0.13 -0.1 253,108 - espY1 MCAODC_01325 0.13 -2.3 253,108 - espY1 MCAODC_01325 0.13 -0.3 253,112 - espY1 MCAODC_01325 0.14 +1.6 253,121 + espY1 MCAODC_01325 0.15 -2.0 253,122 - espY1 MCAODC_01325 0.15 +0.3 253,123 + espY1 MCAODC_01325 0.15 +0.2 253,123 + espY1 MCAODC_01325 0.15 +1.0 253,123 + espY1 MCAODC_01325 0.15 +1.2 253,124 - espY1 MCAODC_01325 0.16 +0.4 253,124 - espY1 MCAODC_01325 0.16 -1.3 253,209 + espY1 MCAODC_01325 0.27 -1.0 253,209 + espY1 MCAODC_01325 0.27 +2.0 253,271 + espY1 MCAODC_01325 0.36 -0.0 253,272 - espY1 MCAODC_01325 0.36 +1.4 253,272 - espY1 MCAODC_01325 0.36 -0.1 253,272 - espY1 MCAODC_01325 0.36 +1.9 253,281 + espY1 MCAODC_01325 0.37 -0.6 253,300 - espY1 MCAODC_01325 0.39 -2.1 253,336 + espY1 MCAODC_01325 0.44 +1.5 253,369 + espY1 MCAODC_01325 0.49 -0.2 253,408 - espY1 MCAODC_01325 0.54 -0.7 253,408 - espY1 MCAODC_01325 0.54 +0.6 253,477 - espY1 MCAODC_01325 0.63 -1.6 253,518 + espY1 MCAODC_01325 0.69 -0.2
Or see this region's nucleotide sequence