Experiment: Control_ECRC101_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafC and dkgB overlap by 4 nucleotides dkgB and Asp_trna are separated by 162 nucleotides Asp_trna and 5S_rrna are separated by 54 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides
MCAODC_00515: yafC - DNA-binding transcriptional regulator YafC, at 82,998 to 83,912
yafC
MCAODC_00520: dkgB - 2,5-didehydrogluconate reductase DkgB, at 83,909 to 84,712
dkgB
MCAODC_00525: Asp_trna - tRNA-Asp(gtc), at 84,875 to 84,951
_trna
MCAODC_00530: 5S_rrna - 5S ribosomal RNA, at 85,006 to 85,121
_rrna
MCAODC_00535: 23S_rrna - 23S ribosomal RNA, at 85,216 to 88,120
_rrna
Position (kb) 83
84
85 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 82.913 kb on + strand at 82.914 kb on - strand at 82.927 kb on + strand at 82.927 kb on + strand at 82.927 kb on + strand at 82.927 kb on + strand at 82.969 kb on - strand at 82.978 kb on - strand at 83.077 kb on + strand at 83.077 kb on + strand at 83.168 kb on + strand, within yafC at 83.194 kb on - strand, within yafC at 83.261 kb on + strand, within yafC at 83.264 kb on + strand, within yafC at 83.264 kb on + strand, within yafC at 83.368 kb on + strand, within yafC at 83.368 kb on + strand, within yafC at 83.369 kb on - strand, within yafC at 83.374 kb on + strand, within yafC at 83.438 kb on + strand, within yafC at 83.513 kb on + strand, within yafC at 83.513 kb on + strand, within yafC at 83.551 kb on + strand, within yafC at 83.598 kb on - strand, within yafC at 83.629 kb on + strand, within yafC at 83.629 kb on + strand, within yafC at 83.629 kb on + strand, within yafC at 83.629 kb on + strand, within yafC at 83.630 kb on - strand, within yafC at 83.644 kb on - strand, within yafC at 83.660 kb on + strand, within yafC at 83.660 kb on + strand, within yafC at 83.661 kb on - strand, within yafC at 83.721 kb on + strand, within yafC at 83.721 kb on + strand, within yafC at 83.721 kb on + strand, within yafC at 83.721 kb on + strand, within yafC at 83.721 kb on + strand, within yafC at 83.723 kb on + strand, within yafC at 83.724 kb on - strand, within yafC at 83.724 kb on - strand, within yafC at 83.724 kb on - strand, within yafC at 83.764 kb on + strand, within yafC at 83.774 kb on + strand, within yafC at 83.774 kb on + strand, within yafC at 83.775 kb on - strand, within yafC at 83.811 kb on + strand, within yafC at 83.814 kb on + strand, within yafC at 83.834 kb on + strand at 83.834 kb on + strand at 83.852 kb on + strand at 83.875 kb on + strand at 83.910 kb on + strand at 83.910 kb on + strand at 83.910 kb on + strand at 83.911 kb on - strand at 84.003 kb on - strand, within dkgB at 84.003 kb on - strand, within dkgB at 84.016 kb on + strand, within dkgB at 84.022 kb on + strand, within dkgB at 84.044 kb on - strand, within dkgB at 84.044 kb on - strand, within dkgB at 84.059 kb on + strand, within dkgB at 84.059 kb on + strand, within dkgB at 84.059 kb on + strand, within dkgB at 84.060 kb on - strand, within dkgB at 84.180 kb on + strand, within dkgB at 84.180 kb on + strand, within dkgB at 84.180 kb on + strand, within dkgB at 84.181 kb on - strand, within dkgB at 84.181 kb on - strand, within dkgB at 84.181 kb on - strand, within dkgB at 84.188 kb on - strand, within dkgB at 84.188 kb on - strand, within dkgB at 84.191 kb on - strand, within dkgB at 84.240 kb on + strand, within dkgB at 84.266 kb on - strand, within dkgB at 84.578 kb on + strand, within dkgB at 84.598 kb on - strand, within dkgB at 84.712 kb on + strand at 84.712 kb on + strand at 84.712 kb on + strand at 84.712 kb on + strand at 84.713 kb on - strand at 84.726 kb on + strand at 84.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101_WM remove 82,913 + +0.5 82,914 - +1.0 82,927 + -0.3 82,927 + +0.2 82,927 + +0.5 82,927 + +1.6 82,969 - +1.7 82,978 - +0.2 83,077 + +1.3 83,077 + -0.8 83,168 + yafC MCAODC_00515 0.19 +1.0 83,194 - yafC MCAODC_00515 0.21 +0.4 83,261 + yafC MCAODC_00515 0.29 +1.8 83,264 + yafC MCAODC_00515 0.29 -0.0 83,264 + yafC MCAODC_00515 0.29 -1.1 83,368 + yafC MCAODC_00515 0.40 +1.3 83,368 + yafC MCAODC_00515 0.40 -1.2 83,369 - yafC MCAODC_00515 0.41 -1.0 83,374 + yafC MCAODC_00515 0.41 -0.1 83,438 + yafC MCAODC_00515 0.48 -1.2 83,513 + yafC MCAODC_00515 0.56 -0.3 83,513 + yafC MCAODC_00515 0.56 -0.3 83,551 + yafC MCAODC_00515 0.60 +0.7 83,598 - yafC MCAODC_00515 0.66 +1.5 83,629 + yafC MCAODC_00515 0.69 +0.9 83,629 + yafC MCAODC_00515 0.69 +0.3 83,629 + yafC MCAODC_00515 0.69 -0.2 83,629 + yafC MCAODC_00515 0.69 +2.8 83,630 - yafC MCAODC_00515 0.69 -0.3 83,644 - yafC MCAODC_00515 0.71 -0.9 83,660 + yafC MCAODC_00515 0.72 +0.1 83,660 + yafC MCAODC_00515 0.72 +0.8 83,661 - yafC MCAODC_00515 0.72 -1.2 83,721 + yafC MCAODC_00515 0.79 -0.6 83,721 + yafC MCAODC_00515 0.79 -2.9 83,721 + yafC MCAODC_00515 0.79 +0.6 83,721 + yafC MCAODC_00515 0.79 -3.1 83,721 + yafC MCAODC_00515 0.79 -2.6 83,723 + yafC MCAODC_00515 0.79 -0.9 83,724 - yafC MCAODC_00515 0.79 -1.5 83,724 - yafC MCAODC_00515 0.79 -1.2 83,724 - yafC MCAODC_00515 0.79 -2.2 83,764 + yafC MCAODC_00515 0.84 +0.3 83,774 + yafC MCAODC_00515 0.85 -0.5 83,774 + yafC MCAODC_00515 0.85 -1.5 83,775 - yafC MCAODC_00515 0.85 -0.4 83,811 + yafC MCAODC_00515 0.89 +0.3 83,814 + yafC MCAODC_00515 0.89 -1.2 83,834 + +1.2 83,834 + -1.4 83,852 + -0.1 83,875 + +0.2 83,910 + +0.2 83,910 + +0.0 83,910 + +0.6 83,911 - +0.4 84,003 - dkgB MCAODC_00520 0.12 +0.2 84,003 - dkgB MCAODC_00520 0.12 -0.2 84,016 + dkgB MCAODC_00520 0.13 +0.4 84,022 + dkgB MCAODC_00520 0.14 +0.2 84,044 - dkgB MCAODC_00520 0.17 +0.1 84,044 - dkgB MCAODC_00520 0.17 +0.1 84,059 + dkgB MCAODC_00520 0.19 -0.4 84,059 + dkgB MCAODC_00520 0.19 +0.7 84,059 + dkgB MCAODC_00520 0.19 -1.7 84,060 - dkgB MCAODC_00520 0.19 -0.7 84,180 + dkgB MCAODC_00520 0.34 +1.4 84,180 + dkgB MCAODC_00520 0.34 +1.1 84,180 + dkgB MCAODC_00520 0.34 +0.2 84,181 - dkgB MCAODC_00520 0.34 +1.4 84,181 - dkgB MCAODC_00520 0.34 +0.5 84,181 - dkgB MCAODC_00520 0.34 -0.6 84,188 - dkgB MCAODC_00520 0.35 -0.2 84,188 - dkgB MCAODC_00520 0.35 +0.3 84,191 - dkgB MCAODC_00520 0.35 -1.9 84,240 + dkgB MCAODC_00520 0.41 +0.3 84,266 - dkgB MCAODC_00520 0.44 +1.9 84,578 + dkgB MCAODC_00520 0.83 +0.3 84,598 - dkgB MCAODC_00520 0.86 +2.4 84,712 + -1.9 84,712 + -1.2 84,712 + +2.1 84,712 + +1.3 84,713 - -2.6 84,726 + +0.2 84,872 - +0.3
Or see this region's nucleotide sequence