Strain Fitness in Escherichia coli ECRC101 around MCAODC_23705

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_23690 and tnpB are separated by 75 nucleotidestnpB and tnpB overlap by 4 nucleotidestnpB and MCAODC_23705 are separated by 49 nucleotidesMCAODC_23705 and MCAODC_23710 are separated by 325 nucleotidesMCAODC_23710 and MCAODC_23715 are separated by 161 nucleotides MCAODC_23690: MCAODC_23690 - DNA-binding protein, at 1,455,850 to 1,456,161 _23690 MCAODC_23695: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,456,237 to 1,456,617 tnpB MCAODC_23700: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,456,614 to 1,456,961 tnpB MCAODC_23705: MCAODC_23705 - IS66-like element ISEc8 family transposase, at 1,457,011 to 1,458,549 _23705 MCAODC_23710: MCAODC_23710 - hypothetical protein, at 1,458,875 to 1,459,144 _23710 MCAODC_23715: MCAODC_23715 - DEAD/DEAH box helicase, at 1,459,306 to 1,461,666 _23715 Position (kb) 1457 1458 1459Strain fitness (log2 ratio) -1 0 1 2 3at 1458.843 kb on - strandat 1458.844 kb on + strandat 1458.845 kb on - strandat 1458.908 kb on + strand, within MCAODC_23710at 1458.944 kb on - strand, within MCAODC_23710at 1458.968 kb on - strand, within MCAODC_23710at 1458.968 kb on - strand, within MCAODC_23710at 1459.104 kb on - strand, within MCAODC_23710at 1459.122 kb on + strandat 1459.143 kb on - strandat 1459.151 kb on - strandat 1459.210 kb on - strandat 1459.216 kb on + strandat 1459.231 kb on + strandat 1459.334 kb on - strandat 1459.358 kb on + strandat 1459.401 kb on - strandat 1459.473 kb on - strandat 1459.480 kb on + strandat 1459.481 kb on - strandat 1459.481 kb on - strandat 1459.481 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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1,458,843 - +2.8
1,458,844 + +0.9
1,458,845 - +0.7
1,458,908 + MCAODC_23710 0.12 -0.1
1,458,944 - MCAODC_23710 0.26 +1.9
1,458,968 - MCAODC_23710 0.34 -0.8
1,458,968 - MCAODC_23710 0.34 -0.5
1,459,104 - MCAODC_23710 0.85 +0.9
1,459,122 + +0.6
1,459,143 - -1.0
1,459,151 - -0.4
1,459,210 - +0.8
1,459,216 + +0.9
1,459,231 + +0.4
1,459,334 - +0.8
1,459,358 + +0.6
1,459,401 - -1.4
1,459,473 - +0.6
1,459,480 + -0.8
1,459,481 - +1.4
1,459,481 - -1.2
1,459,481 - +0.5

Or see this region's nucleotide sequence