Strain Fitness in Escherichia coli ECRC101 around MCAODC_16545

Experiment: Control_ECRC101_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_16535 and MCAODC_16540 are separated by 122 nucleotidesMCAODC_16540 and ompC2 are separated by 168 nucleotidesompC2 and yddG are separated by 258 nucleotides MCAODC_16535: MCAODC_16535 - leucine Rich Repeat family protein, at 202,497 to 203,129 _16535 MCAODC_16540: MCAODC_16540 - IpaH-like protein, at 203,252 to 203,755 _16540 MCAODC_16545: ompC2 - Outer membrane porin C 2, at 203,924 to 205,024 ompC2 MCAODC_16550: yddG - aromatic amino acid efflux DMT transporter YddG, at 205,283 to 206,107 yddG Position (kb) 203 204 205 206Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 203.500 kb on + strand, within MCAODC_16540at 203.881 kb on - strandat 203.926 kb on - strandat 204.114 kb on - strand, within ompC2at 204.114 kb on - strand, within ompC2at 204.208 kb on + strand, within ompC2at 204.222 kb on + strand, within ompC2at 204.287 kb on + strand, within ompC2at 204.337 kb on + strand, within ompC2at 204.640 kb on + strand, within ompC2at 204.641 kb on - strand, within ompC2at 204.866 kb on - strand, within ompC2at 204.951 kb on + strandat 204.983 kb on + strandat 205.477 kb on - strand, within yddGat 205.784 kb on + strand, within yddGat 205.867 kb on - strand, within yddGat 205.975 kb on - strand, within yddG

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101_WM
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203,500 + MCAODC_16540 0.49 -2.7
203,881 - +2.7
203,926 - -0.1
204,114 - ompC2 MCAODC_16545 0.17 -1.1
204,114 - ompC2 MCAODC_16545 0.17 +0.9
204,208 + ompC2 MCAODC_16545 0.26 -0.0
204,222 + ompC2 MCAODC_16545 0.27 +0.2
204,287 + ompC2 MCAODC_16545 0.33 -0.1
204,337 + ompC2 MCAODC_16545 0.38 +0.3
204,640 + ompC2 MCAODC_16545 0.65 -0.1
204,641 - ompC2 MCAODC_16545 0.65 -2.1
204,866 - ompC2 MCAODC_16545 0.86 +0.2
204,951 + +0.2
204,983 + +0.1
205,477 - yddG MCAODC_16550 0.24 +0.6
205,784 + yddG MCAODC_16550 0.61 -2.1
205,867 - yddG MCAODC_16550 0.71 +0.6
205,975 - yddG MCAODC_16550 0.84 +4.0

Or see this region's nucleotide sequence